The reverse cholesterol transport pathway is an important mechanism that prevents the leading cause of heart disease, atherosclerosis. In this pathway, cholesterol is transferred from the peripheral tissues to high density lipoproteins (HDL) that transport cholesterol to either the liver for bile excretion or the steroidogenic tissues for steroid hormone synthesis. Our lab focuses on the critical HDL receptor, Scavenger receptor class B type I (SR-BI), that mediates the selective uptake of cholesterol esters from HDL particles via the interaction between SR-BI and HDLs’ primary protein component, apolipoprotein A-I (apoA-I).
Novel cross-linking strategies coupled with mass spectrometry will help us to better understand the interaction between SR-BI and apoA-I. Proper receptor-ligand binding is essential for cholesterol transport and excretion, so more knowledge of this interaction can lead to therapeutic techniques that lower total plasma cholesterol levels.
Functional genomics of the human HDL receptor scavenger receptor BI: an old dog with new tricks. Chadwick, A.C. and Sahoo, D. Current Opinion in Endocrinology, Diabetes, and Obesity. (2013) Accepted (Ref # MED650)
Functional Characterization of Newly-Discovered Mutations in Human SR-BI. PLoS ONE. Chadwick, A.C. and Sahoo, D. 7(9): e45660. doi:10.1371/journal.pone.0045660 (2012)
CD8+Foxp3+ Regulatory T Cells Are Induced during Graft-versus-Host Disease and Mitigate Disease Severity. Beres, AJ., Haribhai, D., Chadwick, AC., Gonyo, PJ., Williams, CB., Drobyski, WR. The Journal of Immunology.189: 464-474. (2012) doi: 10.4049/jimmunol.1200886