Meetha M. Medhora, PhD
Associate Professor, Medicine (Pulmonary)
Phone: (414) 955-5612
Dr. Meetha M. Medhora's laboratory is studying the effect of ionizing radiation on the vasculature of the lungs. 2) We are also interested in the vaso-reactive and anti-apoptotic actions of EETs and 20-HETE (products of the essential fatty acid, arachidonic acid) on blood vessels.
Effects of ionizing radiation on the vasculature of the lungs
As part of the MCW Center for Countermeasures Against Radiation, we are identifying agents to mitigate radiological injury to the lungs. Using a rat model we have observed a single non-lethal dose of 10-15 Gy to the thorax to result in vascular injury after 6-8 weeks. Specifically in collaboration with the laboratory of Dr. Robert Molthen, an engineer at the Zablocki VA Medical Center, we measured a reduction in density, reactivity, distensibility and endothelial function accompanied by an increase in resistance, in the pulmonary vasculature. All the injuries except for vascular distensibility recover by one year. Currently we are testing a number of pharmaceutical reagents in an effort to identify mitigators and treatment for the radiation pneumonitis we observe.
Vasoreactive and antiapoptotic actions of EETs and 20-HETE (products of the essential fatty acid, arachidonic acid)
The endogenous fatty acids EETs and 20-HETE have potent vasoreactive and other properties. In collaboration with the laboratory of Dr. Elizabeth Jacobs we have demonstrated that EETs protect pulmonary arteries ex vivo using vessels from three species, human, mouse and rat. 20-HETE also protects rat pulmonary and cerebral arteries from hypoxia and reoxygenation injury ex vivo. We are currently studying the intracellular mechanisms for protection by these fatty acids.
Selected Recent Publications
Zhang R, Mio Y, Pratt PF, Lohr N, Warltier DC, Whelan HT, Zhu D, Jacobs ER, Medhora M, Bienengraeber M. (2009). Near infrared light protects cardiomyocytes from hypoxia and reoxygenation injury by a nitric oxide dependent mechanism. J Mol Cell Cardiol. 46(1):4-14. PMID 18930064.
Dhanasekaran A, Bodiga S, Gruenloh S, Gao Y, Dunn L, Falck JR, Buonaccorsi JN, Medhora M, Jacobs ER. (2009). 20-HETE increases survival and decreases apoptosis in pulmonary arteries and pulmonary artery endothelial cells. Am J Physiol Heart Circ Physiol. 296(3):H777-786. PMID 19136601.
Bodiga S, Zhang R, Jacobs DE, Larsen BT, Tampo A, Manthati VL, Kwok WM, Zeldin DC, Falck JR, Gutterman DD, Jacobs ER, Medhora MM. (2009). Protective actions of epoxyeicosatrienoic acid: dual targeting of cardiovascular PI3K and KATP channels. J Mol Cell Cardiol. 46(6):978-988. PMID 19336274.
Sharma M, McCarthy ET, Reddy DS, Patel PK, Savin VJ, Medhora M, Falck JR. (2009). 8,9-Epoxyeicosatrienoic acid protects the glomerular filtration barrier. Prostaglandins Other Lipid Mediat. 89(1-2):43-51. PMID 19480064.
Ghosh SN, Wu Q, Mäder M, Fish BL, Moulder JE, Jacobs ER, Medhora M, Molthen RC. (2009). Vascular injury after whole thoracic x-ray irradiation in the rat. International Journal of Radiation Biology Oncology Physics. 74(1):192-199. PMID 19362237.
Medhora MM, Chen Y, Gruenloh SK, Harland D, Bodiga S, Zielonka J, Gebremedhin D, Gao Y, Falck JR, Anjaiah S, Jacobs ER. (2008). 20-HETE increases superoxide production and NADPH oxidase in pulmonary artery endothelial cells. Am J Physiol Lung Cell Mol Physiol. 294(5):L902-L911. PMID 18296498.
Publications as listed in PubMed