Principal Investigator, Senior Associate Director of the Cardiovascular Center, Northwestern Mutual Professor in Cardiology
Dr. Gutterman is actively involved in clinical practice, supervises a NIH funded research laboratory and provides senior administrative oversight of research administration at the Medical College of Wisconsin. He has served in a leadership role in a variety of national and international cardiovascular scientific organizations.
Research Technologist II
Within Dr. Gutterman’s lab my primary role is to collect and analyze experimental data for multiple research projects and to provide support where needed to meet the goals of the laboratory faculty. I have background in cell biology and regenerative medicine and an interest in genetics and physiology.
Julie Freed, MD, PhD
Within the theme of the Gutterman lab, my interests include understanding the role of the endothelium in vascular health and disease. My current research looks into how biologically active lipids, specifically sphingolipids which are known to be elevated in patients with coronary artery disease, can signal to the mitochondria and alter the mediator of flow-mediated dilation from nitric oxide to hydrogen peroxide. I have also recently become interested in the role of endothelial microparticles in vascular dysfunction.
My research interests examine how acute and chronic stress can alter human vascular function. Using isolated human adipose arterioles, we have shown that increasing intraluminal pressure within the vessel can acutely impair vasodilator responses to acetylcholine and shear stress. This impairment can be reversed in the presence of renin-angiotensin system inhibitors, suggesting that the vascular renin-angiotensin system plays a role in this response. Another study I’m involved with is a collaboration with Marquette University, which examines differential responses to exercise in the femoral artery of people who have suffered a hemiparetic stroke. Preliminary data from this study suggests that decreased blood flow to the paretic leg contributes to baseline muscle weakness and increased neuromuscular fatigability in these individuals.
Research Technologist I
Dr. David Gutterman’s lab uses discarded surgical tissue for functional vessel studies and I am in charge of all the tissue collection related to this research and maintain a database of all the samples collected. I also make various solutions used in these studies and perform many general lab tasks to help the other lab members meet their research goals.
Research Technologist I
In the Gutterman Lab I have two primary roles. First, I act as a lab manager handling administrative aspects such as budgeting, purchasing, managing time cards, and managing IRB and IACUC protocols. Secondly, I provide research support for multiple research projects within the lab. My research work currently involves cell culture, western blotting, and fluorescent imaging work. I graduated with a bachelor’s in biology and I have an interest in physiology, specifically cardiology and nephrology.
As a graduate student in Dr. Gutterman’s lab, I am interested in endothelium-independent vascular pathophysiology. Currently, my focus lays in the area of ion channel-mediated hyperpolarization and relaxation of vascular smooth muscle cells in the microcirculation which is critical in regulating myocardial perfusion. Using cannulated vessels in combination with electrophysiological and molecular techniques, I plan to examine the role coronary artery disease (CAD) has in modulating voltage-activated potassium channel-mediated hyperpolarization of vascular smooth muscle cells and dilation of resistant coronary arterioles.
Natalya Zinkevich, PhD
Research Scientist I
My research interests are related to vascular health and disease. Specifically, I am interested in the role of endothelial cells in mediating vasodilation. Within this broad topic, I address three questions:
How do the mechanisms of endothelium-dependent dilation change during aging and with the onset of coronary artery disease?
What is the role of cellular enzymatic sources of reactive oxygen species in this process?
How does the transient receptor potential vanilloid 4 (TRPV4) channel superfamily contributes to vasorelaxation?
MCW Cardiovascular Center
Medical College of Wisconsin
8701 Watertown Plank Rd.
Milwaukee, WI 53226
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