National Biomedical Electron Paramagnetic Resonance Center

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EPR Services

Service activities at the National Biomedical EPR Center are diverse in nature and include:

  • Provision of engineering drawings, advice, and, in some cases, hardware;
  • Assistance with construction of EPR and sample-handling equipment;
  • Carrying out of HFSS simulations to assist in resonator design;
  • Provision of novel spin traps and synthetic methodology;
  • Recording and analysis of EPR spectra at 1-35 GHz;
  • Spin-labeling services;
  • Providing advice regarding commissioning, testing, and maintaining of cryogenic equipment;
  • Matrix diagonalization computer simulations;
  • Performance of rapid-freeze quench spectrokinetic experiments.

These activities involve the entire complement of faculty and staff at the Center and often graduate students.

To apply for Center Services, use this Service Request Form, contact Brian Bennett, Service Director, directly (, or contact the appropriate staff member for the service categories described below.


EPR Simulation
Spectral simulations using the computer program XSophe.  XSophe is a matrix diagonalization EPR simulation package developed by the Centre for Magnetic Resonance, University of Queensland, Australia, and by Bruker. It is particularly useful for S>1/2 systems, including integer spin systems and spin-coupled systems. Direct values for the inter-metal distances are returned. The program can simulate in both RF perpendicular to H0 and RF parallel modes and at any temperature. A wide variety of line shape models is available, including strains in the Zeeman or zero-field splitting terms. Contact Brian Bennett ( or use the Service Request Form.


Training in EPR spectroscopy and related techniques
.  Training is available for young scientist for up to a two-week visit, with some financial support.  A new type of training for advance visitors is now offered for one to three months.  (See the Training Section)

Training workshops in EPR spectroscopy.   Principles of EPR Training Workshops were held in 2004, 2006, 2009 and 2012.  They are developed to offer practical, specific training for students and postdocs new to EPR technology.  Future training workshops will be held.  Contact Candice Klug ( or Brian Bennett (


EPR Spectrometer Availability
EPR spectrometers are available to experienced spectroscopists for studies that do not require professional staff assistance.  The available EPR spectrometers.  Contact Brian Bennett ( or the faculty member with whom you have previously worked.

EPR Spectra from Samples
EPR spectra from samples submitted by mail will be obtained if the study is routine.  Otherwise, a visit to the Center is more appropriate.  Contact Brian Bennett ( or the faculty member with whom you have previously worked.

Rapid-Freeze-Quench Sample Preparation
We can generate samples with reaction times down to 10 ms, and reaction temperatures at 2 - 37°C, for spectrokinetic studies. Contact Brian Bennett (

Spin Labels and Spin Traps
Advice concerning synthesis of spin labels and spin traps can be obtained from Dr. Joy Joseph. Limited quantities of spin labels and spin traps that have been synthesized by Dr. Joseph are available free of charge from the Center on a case-by-case basis, depending on quantities required and quantities available (contact: Dr. Joy Joseph).

General Advice Concerning EPR Spectroscopy
Contact any faculty or staff member.


Engineering Assistance:  Specialized pieces of EPR Equipment
Assistance is available in making various pieces of specialized EPR equipment, including not only advice but also in many cases mechanical drawings. Examples include the following:

  • Numerous loop gap resonators or cavity resonators as described in our papers (contact: Dr. James S. Hyde)
  • Holder for TPX capillaries in an Eppendorf centrifuge tube

Engineering Assistance:  Technical Information for Maintenance of Varian EPR Spectrometers.
Center engineers, as well as Dr. Hyde, have extensive knowledge of Varian EPR spectrometers. They may be able to help individuals locate parts. If there is a technical question, contact Chief Engineer Joseph J. Ratke.

Engineering Assistance:  EPR Engineering
The very well-equipped microwave laboratory of the EPR Center is open to qualified users (contact: Joseph J. Ratke). Dr. Richard R. Mett has extensive experience in finite-element modeling of electromagnetic field in resonators.

Service Request Form

© 2014 Medical College of Wisconsin
Page Updated 10/14/2014