Posters and Talks
2010
Talks at the Joint Conference of the 14th In Vivo EPR Spectroscopy and Imaging and the 11th International EPR Spin Trapping/Spin Labeling, San Juan, Puerto Rico, May 2-6, 2010
Antholine, W. Multifrequency EPR of Cupric Sites: Four Coordinate, Three Coordinate, and Free Radical Like Sites.
Bennett, B. Rapid-FrUpdateeeze-Quench EPR: What, Why, and How.
Feix, JB. Structure and Conformational Dynamics of the Pseudomonos oeruginosa Toxin , ExoU.
Hyde, JS. Experiments at Low Microwave Frequencies.
Joseph, J. Lopez M, Chen G, Prah D, Ramadoss J, Zielonka J, Kalyanaraman B. Mitochondria-Targeted Nitroxides: Synthesis and Biological Appplications.
Sidabras, J. Mett RR, Hyde JS. Rapid Development of EPR Resonators using Finite-element Modeling
Subczynski, WK. Ragua M, Mainali L. Physical Properties of Lipid Bilayers using Spin Label EPR.
Vasquez-Vivar, J. Whitsett J, Yu L, Ji X, Tan S. Deficient Tetrahydrobiopterin Explains the Double-Hit of Fetal Brain Damage in Hypoxia-Ischemia: A Role for NOS Uncoupling.
Zielonka, J. Detection of Peroxynitrite by Boronate-based Probes: EPR, Fluorescence and HPLC Studies.
Posters at the Joint Conference of the 14th In Vivo EPR Spectroscopy and Imaging and the 11th International EPR Spin Trapping/Spin Labeling, San Juan, Puerto Rico, May 2-6, 2010
Kittell AW, Sidabras JW, Hyde JS. Measuring Dipolar Distances Using Low Frequency (1.8 GHz) Continuous Wave EPR.
Westfahl KM, Merten J, Klug CS. Characterization of the H537A Mutation in the ABC Transporter MSBA.
Zielonda J, Sikora A, Lopez M, Joseph J, Kalyanaraman B. Detection of Free-Radical Intermediates and Products derived from the Reaction Between Boronates and Peroxynitrite-EPR Spin-Trapping and HPLC Studies.
2008
Mett RR, Sidabras JW, Hyde JS. Ferroelectric Inserts in TE011 Cavities for EPR Epectroscopy. 50th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 27-31, 2008
Mett RR, Sidabras JW, Anderson JR, Hyde JS. W-Band Cylindrical TE01 Rectangular TE10 Waveguide Mode Converter. 50th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 27-31, 2008
Sidabras JS, Hyde JS. A Numerical and Analytical Approach for 100 kHz Modulation Coupling into EPR Cavities. 50th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 27-31, 2008
Sidabras JS, Mett RR, Hyde JS. Five-loop-four-gap LGR and AquaStar Sample Holder for Optimization of Concentration Sensitivity.y. 50th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 27-31, 2008
2007
Francis DJ, Klug CS. Investigating the Solution Structure of Visual Arrestin. A Joint Conference of 12th In Vivo EPR Spectroscopy and Imaging and 9th International EPR Spin Trapping/Spin Labeling, Chicago, Illinois, April 29-May 3, 2007.
Mett RR, Sidabras JW, Hyde JS. Iris Coupling of Waveguide to Loop-Gap Resonators at High Frequencies for EPR Spectroscopy. 49th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 22-26, 2007.
Mett RR, Sidabras JW, Hyde JS. Uniform RF Fields in Loop-Gap Resonators for EPR Spectroscopy. 49th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 22-26, 2007.
Sato H, Feix JB. Development and Characterization of Antimicrobial Cecropin-Mellitin Hybrid Peptide, CM15. American Society for Microbiology (ASM) Pseudomonas Meeting, Seattle, Washington, August 27-30, 2007.
Subczynski WK, Kusumi A, Hyde JS. Saturation-Recovery EPR Spin Labeling in Biomembranes: An Overview. A Joint Conference of 12th In Vivo EPR Spectroscopy and Imaging and 9th International EPR Spin Trapping/Spin Labeling, Chicago, Illinois, April 29-May 3, 2007.
Feix JB. Site-Directed Spin Labeling Studies of Antimicrobial Peptide—Membrane Interactions. 49th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 22-26, 2007.
Klug CS. Functional Dynamics of an Inner Membrane Protein. 49th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 22-26, 2007.
Kim J-J, Xia C, Hubbard P, Shen AL, Kasper CB, Feix JB. Topology and Orientation of NADPH-Cytochrome P450 Reductase in the Lipid Bilayer. 49th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colardo, July 22-26, 2007.
Francis DJ, Klug CS. Investigating the Solution Structure of Visual Arrestin. 49th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 22-26, 2007.
Ganeshan G, Pistolesi S, Feix JB. Influence of Membrane Lipid Composition on Bilayer Perturbation by a Cecropin-mellitin Hybrid Antimicrobial Peptide. 49th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colardo, July 22-26, 2007.
Hyde JS, Bennett B, Sidabras JW, Antholine WE. EPR Spectra at 2 GHz for Fragments of Prion Protein Bound to Cu2+: Determination of the Number of Bound Nitrogens. 49th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 22-26, 2007.
Pennington PM, Kittell A, Feix JB, Hyde JS. Experimental EPR Spectra of Nitroxide Spin Labels at L-band. 49th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 22-26, 2007.
Raguz M, Widomska J, Subczynski WK. Oxygen Permeability of the Lipid Bilayer Membrane Made of Calf Lens Lipids. 49th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 22-26, 2007.
EPRBioMed.org: User Groups for the EPR Community
Jason W. Sidabras and James S. Hyde
• Optimization of 100 kHz Field Modulation Slot Geometries to Achieve Uniformity for Use in Electron Paramagnetic Resonance
Jason W. Sidabras, James E. Richie and James S. Hyde
• Resonator Developments
James S. Hyde, Jason W. Sidabras, and Richard R. Mett
2006
W-Band Spectrometer with Multiple Irradiation Arms Tailored for Spin Labeler James S. Hyde, 48th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, July 23-27, 2006.
Precision Sample Tube Holders Suitable for Small Resonators. James R. Anderson, Jason W. Sidabras and James S. Hyde POSTER (pdf)
Uniform rf Fields in Loop-Gap Resonators for EPR Spectroscopy Richard R. Mett, Jason W. Sidabras and James S. Hyde POSTER (pdf)
Iris Coupling of Waveguide to Loop-Gap Resonators at High Frequencies for EPR Spectroscopy. Richard R. Mett, Jason W. Sidabras and James S. Hyde
Saturation Characteristics of Multiquantum EPR at Q-band. Patrick M. Pennington and James S. Hyde
Uniform Field Loop-Gap Resonator for use in in vivo EPR Imaging at 250 MHz.
Jason W. Sidabras, Richard R. Mett, Howard J. Halpern and James S. Hyde POSTER (pdf) Loop-Gap Resonator and Cylindrical TE011 Cavity for Aqueous Samples at 94 GHz.
Jason W. Sidabras, James S. Hyde and Richard R. Mett Physical properties of the lipid bilayer made of the total lipid extract from fiber cell plasma membranes of the calf lens
J. Widomska, J. Dillon, W. K. Subczynski Effects of dipolar, monopolar, and nonpolar carotenoids on physical properties of saturated phosphatidylcholine membranes: EPR spin-labeling study.
J. Widomska, A. Wisniewska, W. K. Subczynski
Subczynski WK. Pulse EPR Discrimination by Oxygen Transport (DOT) Method in Characterization of Membrane Domains. Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland, May 2006.
Subczynski WK. Carotenoids (Macular Xanthophylls) in Eye Retina. Molecular Modeling Group, Faculty of Biochemistry Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland, May 2006.
Anderson, J.R. and J.S. Hyde. Precision Cavity-Insertion System for a Split-Pair Superconducting Magnet with Horizontal Bore. EPR symposium poster given at the 47th Rocky Mountain Conference on Analytical Chemistry, Denver, CO, 3 August 2005.
Antholine, W.E., Bennett, B., Karkheck J., Phadnis P., Subczynski, W.K. Multiexponential fits of saturation recovery curves depend on the degree of axial ligation. 49th Biophysical Society Meeting, Long Beach, CA, February 12-16, 2005.
Bretscher, L.E., Morrell, M.T., Klug, C.S. Characterization of the ArnT transferase protein from Salmonella typhimurium. 50th Annual Meeting of the Biophysical Society, Salt Lake City, UT, February 18-22, 2006.
Bright, M., Kotamraju, S., Kalyanaraman, B. Statins stimulate tumor cell cytotoxicity: role of nitric oxide derived from inducible nitric oxide synthase. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S163, 2005.
Browniowska, K., Kettenhofen, N., Hogg, N. S-nitrosothiol transport in red blood cells. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S97, 2005.
Buchaklian, A.H., Klug, C.S. Site-directed spin labeling EPR spectroscopy studies of the MsbA homodimer Walker A motif. 49th Annual Meeting of the Biophysical Society, Long Beach, CA, February 12-16, 2005.
Buchaklian, A.H., Klug, C.S. Site-directed spin labeling EPR studies of the conserved LSGGQ and H-motifs from MsbA. 50th Annual Meeting of the Biophysical Society, Salt Lake City, UT, February 18-22, 2006.
Carlson, K.A., Francis, D.J., Klug, C.S. Expression and purification of arrestin mutants. 16th Annual Argonne Symposium for Undergraduates in Science, Engineering and Mathematics, Argonne, IL, November 4-5, 2005.
Crowder, M.W., Matthews, M.L., Breece, R.M., Costello, A., Bennett, B., Sigdel, T.K. and Tierney, D.L. Probing the Reaction Mechanism of Co(II)-Substituted VanX. 12th International Conference on Biological Inorganic Chemistry, Ann Arbor, MI, July 31 – August 5, 2005.
Francis, D.J., Hanson, S.M., Gurevich, V.V., Hubbell, W.L., Klug, C.S. Solution structure of a plastic region in visual arrestin using SDSL EPR spectroscopy. 49th Annual Meeting of the Biophysical Society, Long Beach, CA, February 12-16, 2005.
Francis, D.J., Hanson, S.M., Gurevich, V.V., Hubbell, W.L., Klug, C.S. Structural dynamics of arrestin upon rhodopsin binding. 50th Annual Meeting of the Biophysical Society, Salt Lake City, UT, February 18-22, 2006.
Francis, D.J., Hanson, S.M., Vishnivetskiy, S.A., Van Eps, N., Hubbell, W.L., Gurevich, V.V., Klug, C.S. Structural dynamics of arrestin proteins upon receptor binding. ASBMB Experimental Biology Annual Meeting, San Francisco, CA, April 1-5, 2006.
Funk, A.L., Klug, C.S. Structural studies of the arabinose transferase ArnT using site-directed spin labeling EPR spectroscopy. 49th Annual Meeting of the Biophysical Society, Long Beach, CA, February 12-16, 2005.
Gurevich, V.V., Hanson, S.M., Francis, D.J., Vishnivetskiy, S.A., Hubbell, W.L., Klug, C.S. Watching arrestin bind: differential engagement of arrestin residues by different functional forms of rhodopsin. ARVO 2006 Annual Meeting, Ft. Lauderdale, FL, April 30-May 4, 2006.
Hanson, S.M., Francis, D.J., Vishnivetskiy, S.A., Nair, K.S., Slepak, V.Z., Hubbell, W.L., Klug, C.S., Gurevich, V.V. Rhodopsin and microtubules compete for arrestin binding in rod photoreceptors. 2005 ARVO, Fort Lauderdale, FL, May 1-5, 2005.
Hanson, S.M., Francis, D.J., Van Eps, N., Hubbell, C.M., Vishnivetskiy, S.A., Klug, C.S., Hubbell, W.L., Gurevich, V.V. The molecular mechanism and functional role of visual arrestin self-association. ARVO 2006 Annual Meeting, Ft. Lauderdale, FL, April 30-May 4, 2006.
Hanson, S.M., Francis, D.J., Vishnivetskiy, S.A., Raman, D., Van Eps, N., Hubbell, W.L., Klug, C.S., Gurevich, V.V. Arrestin binding to microtubules involves a distinct conformational change. ASBMB Experimental Biology Annual Meeting, San Francisco, CA, April 1-5, 2006.
Huang, J., Basu, S., Huang Z., Hill, J., Shields, H., Azarov, I., Huang, K.T., Hogg, N., King, S.B., Gladwin, M.T., Kim-Shapiro, D.B. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S100, 2005.
Hyde, J.S., Sidabras, J.W. Mett, R.R. Finite-Element Modeling of Fields in Loop-Gap Resonators. EPR 2005, Columbus, OH, September 2005.
Joseph, J., Chi, L., Liu, R., Kalyanaraman, B. Neuroprotection by mitochondria-targeted nitroxide in a mouse model of amyotrophic lateral sclerosis. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S61, 2005.
Kalivendi, S.V., Hillard, C.J., Kalyanaraman, B. Oxidants induce alternatively-spliced form of alpha-synuclein: effect on proteasomal dysfunction and dopamine neuron cell death. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S61, 2005.
Kalyanaraman, B., Zielonka, J., Vasquez-Vivar, J. The confounding effects of light, sonication, and MnTBAP on formation of 2-hydroxyethidium, the product of hydroxyethidine and superoxide reaction, as detected by HPLC-EC. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S13, 2005.
Keszler, A., Hogg, N. Mechanistic study of the nitrite-mediated oxidation of myoglobin: the role of the NO2 radical. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S19, 2005.
Kettenhofen, N., Broniowska, K., Riego, J., Chen, C., Hogg, N. System L amino acid transporter is a universal mechanism for intracellular transport of S-nitrosocysteine. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S101, 2005.
Konorev, E., Kalivendi, S., Kalyanaraman, B. Inactive apoptosome confers resistance of adult cardiomyocytes to the apoptotic action of doxorubicin. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S145, 2005.
Kotamraju, S., Matalon, S., Matsunaga, T., Shang, T., Hickman, J., Kalyanaraman, B. Upregulation of immunoproteasomes by nitric oxide: potential antioxidative mechanism in endothelial cells. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S88, 2005.
Kowalski, J., Antholine, W.E., Tierney, D.L., Bennett, B. Low frequency EPR of Co(II). NIH NCRR/NIBIB P41 Principal Investigator Meeting, Bethesda, MD, June 21 – 23, 2005.
Kowalski, J., Bennett, B. Fulvic Acid Mineral Supplements: Science or Snake Oil? 12th International Conference on Biological Inorganic Chemistry, Ann Arbor, MI, July 31 – August 5, 2005.
Mett, R.R., Anderson, J.R., Sidabras, J.W., Hyde, J.S. EPR field modulation analysis of silver plated graphite resonators. EPR symposium poster given at the 47th Rocky Mountain Conference on Analytical Chemistry, Denver, CO, August 3, 2005.
Mett, R.R., Sidabras, J.W., Hyde, J.S. Eddy current effects on field modulation and in lossy dielectrics in EPR. Invited talk given at the EPR Instrumentation Workshop held at the National Biomedical EPR Center at the Medical College of Wisconsin, Milwaukee, WI, 6 May 2005.
Millhauser, G.L., Chattopadhyay, M., Walter, E., Aronoff-Spencer, E., Gerfen, G.J., Antholine, W.E., Bennett, B. The ever changing shape of the prion protein: EPR identifies three distinct copper binding modes. 47th Rocky Mountain Conference on Analytical Chemistry, Denver, CO, August, 2005.
Periyannan, G., Kumar, M., Bennett, B. Mechanistic Studies on the Aminopeptidase from Vibrio proteolyticus. 12th International Conference on Biological Inorganic Chemistry, Ann Arbor, MI, July 31 – August 5, 2005.
Petering, D.H., Xia, C., Antholine, W.E., Forsterling, H. Disaccharide stabilization of the axial amine in bleomycis: A key factor in dioxygen activation in iron bleomycin. International Chemical Congress of Pacific Basin Societies, Dec. 15-20, 2005 (Pacifichem 2005)
Pieper, G., Nilakantan, V., Johnson, C.P., Roza, A.M., Vasquez-Vivar, J. Regulation by biopterin of nitric oxide production in cardiac alloimmnune activation and in cytokine-activated cardiomyocytes ex vivo. American Transplant Congress. Seattle, May 2005.
Riego, J., Hogg, N. The mechanism of soluble guanylyl cyclase activation by S-nitrosocysteine. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S104, 2005.
Sarna, T., Zielonka, J., Roberts, J., Wishart, J., Kalyanaraman, B. Pulse radiolysis measurements of the rate constants between hydroethidine and superoxide. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S114, 2005.
Semrau, J.D., DiSpirito, A.A., Antholine, W.E. Metal binding by a novel biogenic chalkophore, methanobactin, and the effect on microbial activity. 15th Goldschmidt Conference. Moscow, ID, May 20-25, 2005
Sharma, N., Crawford, P.A., Chandrasekar, S., Tierney, D.L., Costello, A., Yang, K-W., Bennett, B., Crowder, M.W.: Kinetic and Structural Studies on Metallo-b-Lactamase ImiS. 12th International Conference on Biological Inorganic Chemistry, Ann Arbor, MI, July 31 – August 5, 2005.
Shi, Y., Whitsett, J., Konorev, E., Baker, J., Kalyanaraman, B., Vasquez-Vivar, J. Gap between iNOS and COX-2 expression in adult rat cardiomyocytes in response to LPS. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S94, 2005.
Sidabras, J.W., Hyde, J.S. and Mett, R.R. Optimization of W-band resonators for use with EPR spectroscopy of aqueous samples. EPR symposium poster given at the 47th Rocky Mountain Conference on Analytical Chemistry, Denver, CO, August 3, 2005.
Vásquez-Vivar, J., Martásek, P., Joseph, J., Kalyanaraman, B. Spin trapping of superoxide from uncoupling of nitric oxide synthase. EPR 2005, Columbus, OH, September 2005.
Vishnivetskiy, S.A., Hanson, S.M., Francis, D.J., Hubbell, W.L., Klug, C.S., Gurevich, V.V. Structural dynamics of visual arrestin-rhodopsin binding. ARVO 2006 Annual Meeting, Ft. Lauderdale, FL, April 30-May 4, 2006.
Vishnivetskiy, S.A., Hanson, S.M., Francis, D.J., Kusnetszow, A.K., Klug, C.S., Hubbell, W.L., Gurevich, V.V. Arrestin-rhodopsin interface: the implications for the structure of the complex. 2005 ARVO, Fort Lauderdale, FL, May 1-5, 2005.
Watanabe, N., Zmijewski, J., Le Goffe, C., Landar, A., Takabe, W., Murphy, M., Kalyanaraman, B., Noguchi, N., Darley-Usmar, V. Activation of map kinases by lysophosphatidylcholine-induced mitochondrial reactive oxygen species generation in endothelial cells. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S80, 2005.
Whitsett, J., Martasek, P., Zhao, H., Kalyanaraman, B., Vasquez-Vivar, J. Superoxide generation, eNOS-serine 1179 and eNOS dimer/monomer ratio in endothelial cells. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S28, 2005.
Zhang, H., Xu, Y., Joseph, J., Kalyanaraman, B. Intramolecular electron transfer between tyrosyl radical and cysteine residue inhibits tyrosine nitration and induces thiyl radical formation in model peptides treated with MPO, H2O2, and NO2-: EPR spin trapping studies. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S16, 2005.
Zielonka, J., Vasquez-Vivar, J., Kalyanaraman, B. Detection of intracellular superoxide with hydroethidine and MitoSOXTM. Presented at the Society of Free Radical Biology and Medicine 12th Annual Meeting, Austin, TX, November 16-20, 2005. Free Radic Biol Med 39 (Suppl 1):S115, 2005.