Perkin-Elmer LS 55 Luminescence Spectrometer
This luminescence spectrometer is a very versatile instrument that allows users to measure fluorescence, phosphorescence, and chemi- or bio-luminescence. The monochromator uses a high energy pulsed xenon lamp to minimize photo-bleaching of samples and to provide a long-lived excitation source. An interchangeable well-plate accessory allows the user to measure samples in 6-96 well-plates, cuvettes, TLC plates, electrophoresis gels, or any other flat sample type.
ESA HPLC system with electrochemical CoulArray® detector
The HPLC system with electrochemical detector enables identification and quantification of electroactive compounds (antioxidants, amines, quinones etc.). The system consists of 2 pumps and high pressure mixer enabling gradient elution, thermostated autosampler, and an electrode array detector. The detector involves 8 coulometric, working independently, porous graphite cells connected in a series. The system offers both high sensitivity and selectivity towards the redox active compounds.
Applied Photophysics Stopped-Flow Spectrophotometer
This instrument (Applied Photophysics, SX18.MV) is used for the kinetic analysis of fast reactions. In general conventional UV-Vis spectrophotometry cannot measure the kinetics of reactions that occur in less than 10 seconds. This is because it takes at least 2 seconds to mix the components and place them in the instrument for analysis referred to as the dead-time). The stopped-flow, using a combination of high-pressure gas-driven syringes and mixing technology, has a dead time of less than 2 ms and so is able to measure accurate kinetics of reactions that occur in as little as 10 ms. The instrument is able to work in both single- and sequential mixing mode. The detection capabilities involve absorbance (photomultiplier or photodiode array) and fluorescence (cut off filters or a second monochromator for choosing emission wavelength range) measurements.
FT-IR Spectrometer Perkin Elmer (Spectrum GX system)
FT-IR, or Fourier Transform Infrared, spectroscopy is a powerful technique for identifying the molecular composition of many organic and inorganic materials. Basically, the spectrometer measures frequencies at which the sample absorbs the radiation and the intensities of the absorptions. Determining these frequencies allows identification of the sample's chemical makeup, since chemical functional groups are known to absorb light at specific frequencies.
Sievers Nitric Oxide Analyzer
This instrument measures very low amounts of nitric oxide using chemiluminescence detection. The basic concept is that nitric oxide reacts with ozone to produce an electronically excited state of nitrogen dioxide, which relaxes back to ground state by giving up light. Depending on the content of the purge vessel, nitric oxide, nitrite, nitrate and S-nitrosothiols can all be measured at pM concentrations.
Gas Chromatograph Varian (Model 3700)
This instrument is dedicated to the measurement of nitrous oxide (N2O or laughing gas), which may be formed from reductive metabolism of nitric oxide.
Gel Documentation System Alpha Innotech Corp. (AlphaImager 2200)
The gel documentation system includes a high-performance CCD camera (zoom lens 12.5-75mm with close-up +2 diopter lens), MultiImage Light Cabinet and thermal printer for high quality print-outs. The light cabinet allows for transillumination with UV and white light for fluorescence and colorimetric stains. It also has epi-illumination for analysis of blots, films or plates. The AlphaeaseTM software features a movie mode, on-screen annotation, 1D-multilane analysis and spot densitometry.
High Performance Liquid Chromatography (HPLC) Agilent Technologies (1100 series)
HPLC is the technique of choice for the identification, quantification, and resolution of many small molecules involved in free radical research including vitamin E,vitamin C, glutathione, nitrotyrosine, dityrosine etc. This particular HPLC is equipped with a thermostatted column compartment, diode array detector, fluorescence detector, quaternary pump, and thermostatted well-plate autosampler. This HPLC is primarily used in the purification of proteins.
UV-Visible Spectrophotometer Agilent Technologies (Model 8453)
This machine is a single-beam microprocessor controlled, UV- visible spectrophotometer with collimating optics. It has diode-array technology which allows it to perform much faster with much more precision, sensitivity, and reproducibility. This machine has a temperature controlled multicell (8 position) transport which can be used for enzyme kinetic studies. It has a wavelength range of 190-1100nm, a slit width of 1nm, and a minimum scan time of 0.1 seconds.
Capillary HPLC Agilent Technologies (1100 series)
This capillary HPLC provides the user the ability to quantify very small sample volumes with greater sensitivity, increased chromatographic resolution, and reduction of solvent consumption. This HPLC is equipped with a micro degasser,capillary pump, multiwavelength detector, and thermostatted autosampler. The pump allows flow rates from 1µL/min to 100µL/min, and the micro autosampler allows injection volumes from nL to µL ranges.
Elexsys Electron Paramagnetic Resonance System--Bruker
This machine is a state-of-the-art EPR instrument that can be used to detect directly free radicals. The instrument will is housed in the National EPR Center in the Biophysics Research Institute (2nd floor of the MACC Fund building).