Shared Mass Spectrometry (MSMS) Facility – Small Molecules
The MSMS Facility is an interdepartmental research service unit managed on behalf of the Medical College of Wisconsin by the Department of Pharmacology and Toxicology. The facility provides service and consultation for research projects requiring mass spectrometric analysis (fundamental, identification and quantitation) of a variety of compounds. The facility operates on a fee for service basis and is open for faculty of the Medical College of Wisconsin and outside researchers. The MSMS Facility has three mass spectrometers with different configuration available for researchers' needs in analysis of their samples.
The MSMS Facility has various mass spectrometers with different configuration available for researchers' needs in analysis of their samples.
Triple Quadrupole-LC Mass Spectrometer
The Agilent 6460 Triple Quad LC/MS/MS consists of electrospray ionization (ESI) source and triple quadrupole mass analyzer. The system is interfaced to a 1290 Infinity liquid chromatograph and autosampler for fast chromatography. The mass analyzer has an upper range of 3000 m/z in both positive and negative detection modes and a data-sampling rate of 150 SRMs per second. The 6460 routinely achieves fmol sensitivity and it is suitable for the qualitative and quantitative determinations of small molecules such as lipids, steroids, drugs and metabolites, nucleic acids, and peptides. The fast data sampling allows rapid analysis of the most complex samples.
Triple Quadrupole-LC Mass Spectrometer
The Waters Quattro Micro APCI Mass Spectrometer (LC/MS/MS) consists of Z-spray source for electrospray ionization (ESI) and chemical ionization (APCI) with the capability of performing a variety of CID experiments. The analyzer has a mass range up to 2000 m/z in both positive and negative detection modes. The Quattro is interfaced to a Waters Alliance 2695 liquid chromatograph and is used for qualitative and quantitative analysis of lipids, steroids, drugs and metabolites, nucleic acids, and peptides at pmol levels.
Triple Quadrupole-GC Mass Spectrometer
The Thermo Scientific TSQ 8000 Mass Spectrometer (LC/MS/MS) is equipped for electron impact (EI) and chemical ionization (CI) with the capability of performing CID experiments. The analyzer has a mass range up to 1100 m/z in both positive, negative and pulsed positive-negative detection modes. The TSQ is interfaced to a Trace 1310 gas chromatography with cooled TriPlus RSH autosampler and is used for qualitative and quantitative analysis of smaller, more volatile lipids, steroids, drugs metabolites, and oxysterols at fmol levels.
Guidelines for Mass Spectrometry Services
- Facility personnel are available to meet and discuss the possibility of utilizing the mass spectrometry for your research projects.
- When it is possible please find protocols for sample preparation and experimental design. We will provide some suggestions if needed.
- A request form (PDF) must be completed explaining the sample type, number of samples, length of project, sample purity, estimated concentration range, what type of analysis, etc.
- The cost of the analyses will be covered by fee-for-service. If column is needed that is not already in the facility then the user may be required to purchase the column. The cost of internal standards will be charged in full if the facility does not feel that other users will need a similar assay. When extensive development time is required the user is expected to pay these costs.
- You will be charged for all samples and standards analyzed.
- If you have no current funding, you may request assistance in obtaining preliminary data for your proposal. If needed, the facility will provide a brief protocol of the MS analysis for your proposal and assist you with budget information for MS analysis.
Charge per sample: $11/each
MS run time: $42/hour
Sample workup and/or setup: $42/hour
Data analysis and report: $42/hour
Supplies (nozzles/tips): $2/each
Administrative costs: $8/billing