Pediatrics: Gastroenterology

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Recommended Protocol for Pancreatic Stimulation Test

Specimens collected via P catheter inserted through biopsy channel. Each specimen should be >0.5 ml if possible. Specimens should be placed on ice immediately, then frozen and stored at -75ºC in an ultra-low freezer until ready to ship.

  1. Baseline collection (T0)
  2. Test dose of synthetic secretin (0.2 mcg) IV over 1 minute.
  3. Full dose of synthetic secretin (0.2 mcg/kg) and CCK (Kinevac) (0.02mcg/kg) IV over 1 minute. Synthetic human secretin (ChiRhoStim) is available from ChiRhoClin (1-877-272-4888)
  4. Specimens collected at 0, 5, 10, 15 minutes following secretin and  CCK (T0, T5, T10, T15). We recommend using a duodenal aspiration catheter.
  5. Each specimen must be at least 0.2 ml.
  6. We will interpret results when samples are obtained as outlined in this protocol. If the protocol is not followed, we will continue to provide the results of pancreatic enzyme measurements but will be unable to interpret results.

Normal Values for individual pancreatic enzymes*:

  • Lipase > 146.0 moles/ml/min.
  • Amylase > 32.0 moles/ml/min.
  • Trypsin > 55.4 nmoles/ml/min.

*Pancreatic function can only be considered as normal when the pre-stimulated or 1 of the post-stimulated aspirates (usually 5-10 mins) attains respective levels of activity as indicated.

Pediatric GI and Nutrition Lab


Mark Struve

Nita Salzman M.D. PhD
© 2014 Medical College of Wisconsin
Page Updated 07/22/2013