Course Director: Brian Volkman
Days Offered: MW 9:00 AM-10:30 AM Location: C 2195
With complete sequences for the genomes of human and many other species now available, much of the attention in molecular biological research is rapidly turning to the characterization of organism-wide collections of gene products, often referred to as functional genomics or proteomics. Just as the human genome project catalyzed generational advances in DNA sequencing technology, current trends demand improved methods for efficient cloning, production and physical characterization of recombinant proteins. Many of these proteins represent undiscovered targets for intervention in human disease, and analysis of their structure and thermodynamics will enable translational research in medicine and drug discovery. This course will focus on the practical aspects of protein production and characterization, including the steps most commonly encountered in the development of targets for three-dimensional structural characterization. New methods and optimization of standard approaches for high-throughput applications will be emphasized, including techniques ranging from chromatography and electrophoresis to mass spectrometry, fluorescence, X-ray crystallography and NMR spectroscopy. Where applicable, their application to the design or discovery of novel ligands, inhibitors, and lead compounds will be emphasized.