Interdisciplinary Program Faculty
EPR spectroscopy to determine the ligation of metals in (primarily, copper) proteins and complex biological systems
Molecular and functional characterization of receptors for the endogenous nucleoside adenosine and the potential for cardiac regeneration following myocardial infarction using embryonic stem cells
Understanding the mechanisms by which adaptation of the heart to chronic hypoxia increases resistance to subsequent ischemia
Mechanisms of Bacterial Toxin Action
Transcriptional control of liver and gut development and physiology
How cells respond to stress is a fundamental property underlying proteostasis, specialized functions (e.g., contraction/relaxation), and disease onset and/or progression. Our laboratory focuses on the cellular mechanisms related to redox biology and mitochondrial function in protein misfolding and related cardiac disorders using complementary models systems such as genetically engineered rodents and human induced pluripotent stem (iPS) cells.
Our long-term goal is to understand how small GTPases can be manipulated to enhance cancer treatment
Cellular and molecular basis or circadian rhythmicity; the cellular basis of membrane turnover in retinal photoreceptors
My laboratory studies the genetics of bone biomechanical performance and vertebral patterning. Endothelin converting enzyme is a strong candidate gene for a quantitative trait locus for bone size and strength we identified in previous work. This has led us to study of endothelin biology in greater detail. As a consequence, we are now also engaged in vascular and reproductive biology as well as bone biology.
Mechanisms and sites of action in the human brain of cocaine, nicotine and marijuana
Regulation of vascular tone and adrenal steroidogenesis by endothelial factors
Structure-function relationships in the human retina, high-resolution retinal imaging, mechanisms of retinal degeneration
Mechanisms by which Ras induces p38gamma expression and by which p38gamma is required for Ras-induced transformation/invasion
Immunogenetics of Type 1 Diabetes
We study function of small GTPases in the cardiovascular system using cell biology, biochemistry and in vivo approaches
Molecular mechanisms underlying liver development and function, with a particular focus on the role played chromatin structure in the translational regulation of liver genes
Pathogenic mechanisms of spirochetes that cause Lyme disease and leptospirosis
Diabetes, beta-cell biology, inflammation innate immunity, cell signaling, cell fate decisions
Transcriptional regulation of effector and memory T cell differentiation during infection and tumorigenesis
Glycobiology, lysosome biogenesis, lysosomal storage disorders, regulation of growth factor and protease activation
Immune regulation of central nervous system autoimmunity
Immunobiology of bone marrow transplantation
Mucosal immunity; immune regulation in cancer
Neurodegenerative diseases, both understanding the molecular basis for the disease progression and finding effective experimental therapies
Elucidation of the effects of sleep deficiency on metabolic, endocrine, and inflammatory processes. Discovery of the specific physical properties that account for restoration by sleep.
Biological and biochemical characterization of antimicrobial peptides, EPR site-directed spin labeling, and the structure and function of member proteins
Genetic regulation of exotoxin synthesis
Mechanistic study of gene transcription and its regulation in eukaryotic cells using structural and biochemical methods
Cellular and molecular mechanisms of synaptic plasticity
Elucidation of pro-survival vs. pro-death signaling cascades in tumor cells and other mammalian cells exposed to oxidative challenges, including therapeutic challenges, with a special interest in nitric oxide-mediated pro-survival signaling
Therapeutic potential of mesenchymal stem cells in brain injury and neurodegenerative disorders
The Grayson Lab is studying how viral respiratory infections drive the development of allergic diseases, like asthma, and how the microbiome affects the respiratory immune system
Interface of stem cell biology and analytical protein biochemistry to better understand pluripotent stem cells and their derivatives in context of cardiac development and disease
Vascular health and disease
Type 1 diabetes, innate immunity, autoimmunity, functional genomics
Druggability of proteins involved in mitochondrial homeostasis in healthy and diseased cells to identify molecular mechanism and novel therapeutic routes
Biochemical mechanisms involved in the synthesis, release, and degradation of the endocannabinoids, the role of endocannabinoid signaling in the regulation of mood and responses to stress and their effects on metabolic functions in the brain
Mechanisms by which nitric oxide affects cellular functions at the level of individual protein modifications
Development of EPR instrumentation and extension of ways in which existing EPR and MRI can be used for new categories of biomedical problems
Elucidation of mechanisms by which eicosanoid metabolites influence cardiovascular function
Transcription within a eukaryotic cell is not process in which RNA polymerase can freely access the DNA for either initiation or elongation of the transcript
Leveraging molecular and cellular technologies, mouse models, and clinical specimens, my research focuses on understanding signaling pathways in tumor initiation, tumor progression, and therapeutic response and resistance with particular emphasis on prostate cancer and skin cancer
Cancer immunotherapy and hematopoietic stem cell transplantation immunology
Application of EPR in free radical biology and understanding the role of free radicals in signal transduction and apoptosis
Structure-function relationships of enzymes and receptors by X-ray crystallography combined with various biochemical methods
Site-directed spin labeling EPR spectroscopy technique to study the functional dynamics of bacterial membrane and retinal proteins
Signal transduction and antibiotic resistance in gram-positive bacteria
Electrophysiology of inherited cardiac arrhythmias; role of ion and nucleotide transport across the outer mitochondrial membrane in cardioprotection
Understand the molecular and cellular mechanisms of synaptic plasticity
Interactions between sensory nerves, mast cells, and fibroblasts in the heart and the role that these interactions play in the development of heart failure
Genetics and cell biology of ocular development and disease
Understand the mechanism of cocaine addiction
RNA localization in yeast
Cell and molecular biology of early heart development
Molecular mechanisms of integrin activation
My laboratory studies the development and functions of natural killer (NK ) cells. Our long term goal is to specifically augment the cytotoxic potentials of human NK cells and to successfully kill and clear tumor in patients.
RNA processing (splicing and polyadenylation) in cells and viruses
Our current research has been focused on the molecular mechanisms of a new ligand-receptor pair, Nogo-B and Nogo-B receptor (NgBR), in regulating stem cell differentiation and blood vessel formation during embryo development and tumor growth, and developing therapeutic approaches to modulate Nogo-B/NgBR functions in vitro and in vivo.
My laboratory seeks to understand how the cell division cycle is interconnected with other fundamental pathways that control cell fate and function.
Profiling protein signatures in glioblastoma multiforme with differential response to bevacizumab (anti-VEGF) therapy useful for early diagnosis and better prognosis of the disease and to identify new therapeutic targets for individualized treatment
Transcriptional control and signaling during epicardial and early coronary vascular development
Elucidation of the role of cytochromes and other electron-transporting proteins in reductive metal metabolism
Signal transduction in platelets and leukocytes, thrombosis, non-receptor tyrosine kinases and phosphatases
Cell adhesion and signaling in blood and vascular cells
Brain mechanisms in drug and non-drug addictive behavior
Elucidation of the innate tumor-suppressive mechanisms that activate senescence or cell death responses when oncogenic alterations occur in cells
Sex, vascular tone and arachidonic acid metabolism and thromboxane receptors in atherosclerosis
My laboratory investigates mechanisms by which vascular inflammation impairs vasodilatation and oxidative stress alters cell signaling to impair vascular function. Another major focus of my is developing novel tools/agents to inhibit key mediators of inflammation and oxidative to improve vascular function
The Developmental Vascular Biology program investigates the basic mechanisms of blood vessel formation in vertebrates and the contribution of the vasculature to disease states. In particular, vascular conditions associated with children such as hemangiomas and solid tumors will benefit because ongoing studies in this program utilize developing zebrafish and mouse embryos.
Transcriptional Regulation of Stem Cells
Biochemistry of inhibitory and stimulatory signal transduction in T cells and application to adoptive T cell therapies
The effects of actual and simulated spaceflight uploading on neuromuscular structure and function and understanding vibration injury of tissues
Cellular responses to mycobacterium tuberculosis
Our laboratory seeks to understand the etiology and optimize the treatment of the unique interstitial lung disease that occurs in patients with common variable immunodeficiency, looks to develop and implement novel assays that can be used in high-throughput population based screening to detect immunodeficiencies in newborns and to develop novel tumor vaccines that by harnessing the immunogenicity of adenovirus E1A
Understanding HDL functionality, mechanisms that regulate the selective uptake of HDL-cholesterol and reverse cholesterol transport, the role of scavenger receptors in uptake of HDL-cholesterol, the relationship between scavenger receptor expression and changes in membrane lipids, the role of CD36 in the uptake of very long chain fatty acids
Innate mucosal immunity, host-pathogen-microbiota interactions in the GI tract, the role of the intestinal microbiota in health and disease
Elucidation of the neuroprotective mechanisms of ubiquitin and chaperone pathways in neurodegenerative diseases
Discovery of genes underlying complex developmental disorders with an emphasis on developmental glaucoma
Molecular genetics of hereditary eye disease
We study the molecular and cellular pathogenesis of common vascular disorders, including thrombosis, atherosclerosis and cancer angiogenesis. We focus on the role of a specific cellular receptor, CD36, expressed on platelets, macrophages, capillary endothelial cells and adipocytes, using mouse models of vascular disease and in vitro models with human cells, with a particular interest in characterizing receptor-ligand interactions, intracellular signaling pathways, and genetic regulatory pathways
Epigenetic and redox control of protein function through post-translational modifications
My laboratory is interested in identifying novel genes involved in the development and progression of type 2 diabetes. To this end, we use an outbred rat model for fine-mapping genetic loci, followed by sequencing and expression to narrow candidate genes.
Maternal/fetal health: Roles of coagulation and platelets in placental vascular disease and maternal hypertensive disorders of pregnancy; estrogen and coagulation cross-talk; biomarkers of pregnancy complications
My lab studies the molecular and cellular mechanisms related to abnormalities in lipid metabolism and the human diseases they cause. Our approach is multi-faceted, involving structural biology, cell biology, immunology and mass spectrometry.
Dr. Sorokin's research is focused on mechanisms of initiation and progression of proliferative kidney diseases. Research in his laboratory is primarily focused on 1) understanding the role of endothelin signaling in hypertension induced nephropathy and proliferative glomerulonephritis; 2) cellular regulation of prostaglandin production with an emphasis on regulation of Cox-2 activity on the level of catalysis by proteins spatially co-localized with the enzyme in its natural environment; 3) elucidation of BK virus entry pathways and prevention of polyomavirus nephropathy after renal transplantation
The Strande Lab is using induced pluripotent stem cells (iPSCs) to study dystrophic cardiomyopathy, which is a type of heart failure that patients with Duchenne or Becker muscular dystrophy develop
Molecules that sense touch and pain
Formation of raft domains in model and biological membranes
Virus-host interaction: manipulation of the DNA damage response by gammaherpesviruses and herpesvirus kinases, regulation of viral chromatin, mouse models of human DNA damage response-associated diseases
Human cytomegalovirus-host cell protein interactions during infection
Oncology drug development and in the treatment of gastrointestinal cancers.
Elucidation of the role of proteinases and proteinase inhibitors in corneal wound healing and corneal ulceration and exploration of the effects of maspin in corneal wound healing and in carcinogenesis
Superoxide generation from eNOS, regulation of NO production in the heart, and tetrahydrobiopterin in fetal brain development
Defects in the immune system.
NMR and crystallographic analysis of ligand binding and conformational dynamics in chemokines and other signaling proteins; structure-based drug discovery of novel anti-metastatic compounds
Study signal transduction pathways that regulate hematopoietic stem cell and B cell biology
Immune regulation mediated by immune-checkpoint regulators; Cancer immunotherapy; cancer vaccine development
Human genome/epigenome variations and cancers, focusing on biomarker study for personalized medicine through large scale RNA/DNA sequencing analysis
My research focus is using human cancer models in pre-clinical setting to study the efficacy of drugs and/or vaccines in cancer prevention.
Cardioprotection (preconditioning and postconditioning) against ischemia and reperfusion injury by pharmacological agents including volatile anesthetics, neovascularization of ischemic myocardium by coronary collateral vessels, and actions of anesthetic agents and positive inotropic agents on left ventricular mechanisms in the absence and presence of disease states such as congestive heart failure
Coronary collateral growth and impaired angiogenesis in diabetes
The overall goal of the White lab is to understand the signaling pathways that mediate the hemostatic responses of blood platelets.
Regulatory T cell development and function
Biochemical signaling pathways and cellular processes regulated by the Ras and Rho families of small GTPases
Coupling of neuronal activity and energy metabolism at the cellular and molecular levels. Critical period of neurochemical and metabolic development in brain stem respiratory neurons. Photobiomodulation of neurons functionally inactivated by toxins
Identification of candidate lung cancer susceptibility genes by capture-sequencing of targeted regions or jeans and frequent somatic mutations in lung adenocarcinomas and squamous cell carcinomas by exome sequencing
Bacterial pathogenesis and host-cell interactions
Study the mechanism of receptor transmembrane signaling using structural, biochemical, and cell biology methods. Structure-based design of small molecules for disturbing receptor function.
My laboratory studies the epigenetic regulation of stem cells and how dysregulation of these pathways contributes to cancer development