Cancer Center

Cancer Center

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Clinical and Translational Research Core Laboratory 

The Medical College of Wisconsin Cancer Center Clinical and Translational Research Core Laboratory (CTRL) performs histological and molecular analysis of human specimens. The major focus of the work is on human cancer.  The lab is CLIA certified allowing it to process clinical samples as well as research projects.

Rates, Location & Hours 

  • The CTRL is located in Froedtert Hospital, 9200 W Wisconsin Ave, Room 1176 FMLH West. 
  • Daily hours of operation are from 8:00 AM to 5:30 PM. 
  • For questions and to schedule services, please contact Deyanka Ogrizovic at dogrizov@mcw.edu or 414.805.1524.   The fax number is 414.805.1528

Services

 

Histology Core Lab

Molecular Core Lab
  • Gross description and analysis of tissue specimens
  • Gross dissection of specimens (MOPEC)
  • Slide printer with barcoding (Thermo SlideMate)
  • Cassette printer with barcoding (Thermo PrintMate)
  • Tissue processing (Sakura VIP6)
  • Tissue paraffin embedding (Sakura TEK6)
  • Tissue sectioning to make slides (Thermo HM 355S)
  • Frozen sections (Thermo HM 525)
  • Slide staining (Leica Autostainer)
  • Immunohistochemical analysis (DAKO Autostainer XL)
  • Immunofluorescence (Olympus BX61)
  • Special staining
  • Tissue microarray construction (Veridiam)
  • Quantitative image analysis (DAKO ACIS III)
  • Laser capture microscopy (Arcturus)
  • Pathological interpretation
  • Tumor Identification 
  • Nucleic acid purification (Qiagen BioRobot EZ1)
  • Nucleic acid quantification (Nanodrop; Qbit)
  • Agilent Bioanalyzer
  • PCR (ABI Veriti)
  • Real time PCR (Lightcycler; ABI 7500)
  • Reverse transcriptase PCR
  • Quantitative PCR (Lightcycler; ABI 7500)
  • Dideoxy sequencing (ABI 3500)
  • Pyrosequencing (Biotage PSQ 96MA)
  • DNA fragment analysis (ABI 3500)
  • DNA microarray analysis (Innopsys Scanner)
  • Ozone-free working environment (SciGene)
  • Gene expression microarray analysis (Innopsys)
  • Gel electrophoresis (Agarose, acrylamide)
  • Fluorescence in situ hybridization (FISH)

Resource Director 

A. Craig Mackinnon, Jr, MD, PhD (Craig) is a board certified anatomical pathologist with sub-specialty board certification in molecular genetic pathology (MGP).  His research interests are molecular cancer diagnostics and biomarker discovery.  

Key Technical Staff 

  • Xiuxu Chen, PhD, Research Scientist II (Pathology Technologist)
  • Min Le, PhD, Research Scientist II (Molecular Technologist)
  • Deyanka Ogrizovic, Administration III
  • Kathryn Stoll, HT(ASCP) (Histologist) 

 

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© 2013 Medical College of Wisconsin
Page Updated 02/19/2013