Facility instrumentation includes a FACS Calibur and a FACS Aria Special Order System(SOS).
The FACS Calibur was purchased in 2000 and is a two-laser (488nm and 615nm), four-color, six-parameter, bench-top analyzer used primarily for evaluating surface and intracellular antigen expression, apoptosis detection, bead-based cytokine detection and cell-cycle analysis. Users are able to run their own samples on this instrument after training with the facility director.
The FACS Aria SOS is a fully-digital, four-laser (405nm, 488nm, 561nm and 640nm), ten-color, twelve-parameter instrument. It was purchased in 2010 by the Cancer Center. It is used for all cell sorting experiments (up to four populations simultaneously) and for immunophenotypic analysis requiring more than four colors. It is also equipped with an aerosol management option and temperature control.
Tamara Nelson, MS, Research Associate
Research Associate returned to MCW in 2013. She earned her graduate degree from
the University of Wisconsin-Madison Biotechnology in 2007. With over ten years of research experience in both academic and biotech industry, she is well versed in many molecular biology and immunochemical techniques. Ms. Nelson provides training for new users, schedules and executes cell sorting, maintains and calibrates the instruments, and manages data archiving for the shared facility. She has completed coursework at BD Biosciences to help troubleshoot sorting rare cell types, small and large cells, as well as maximizing sort optimization and cell recovery.