Facility instrumentation includes a FACS Calibur and a FACS Aria Special Order System(SOS).
The FACS Calibur was purchased in 2000 and is a two-laser (488nm and 615nm), four-color, six-parameter, bench-top analyzer used primarily for evaluating surface and intracellular antigen expression, apoptosis detection, bead-based cytokine detection and cell-cycle analysis. Users are able to run their own samples on this instrument after training with the facility director.
The FACS Aria SOS is a fully-digital, four-laser (405nm, 488nm, 561nm and 640nm), ten-color, twelve-parameter instrument. It was purchased in 2010 by the Cancer Center. It is used for all cell sorting experiments (up to four populations simultaneously) and for immunophenotypic analysis requiring more than four colors. It is also equipped with an aerosol management option and temperature control.