Flow Cytometry Facility

The Medical College of Wisconsin Cancer Center Flow Cytometry facility is a state-of-the art facility that serves investigators at the Medical College of Wisconsin. In addition, the facility serves investigators at the Blood Research Institute, University of Wisconsin-Milwaukee and Marquette University. The Flow Cytometry facility is supported in full by the MCW Cancer Center, through a grant from Advancing a Healthier Wisconsin (AHW). This shared resource provides investigators with on-site, cost-effective, comprehensive analytic flow cytometry and high-speed cell sorting services and expertise.

Rates, Location & Hours


  • The Flow Cytometry facility is located on the 5th floor of the MACC Fund Research Center.
  • The daily hours of operation are from 8:30 a.m. to 4:30 p.m. After-hours and weekend services are available upon request.
  • Flow sorting and cytometer use is billed in half-hour increments, when a cost center has been established.
    Subsidization applies for MCWCC members based on relative support for FLOW operation costs. Current campus rates at the CRI-MCW and BRI facilities are:
    • FACSAria (sorting): $50.00 per hour
    • LSR II: $25.00 per hour
    • FACSCalibur: $10.00 per hour
  • For any questions and to request service, please contact facility manager Tamara Nelson at (414) 456-4300.
 Instrumentation & Services

Facility instrumentation includes a FACS Calibur and a FACS Aria Special Order System(SOS).

The FACS Calibur was purchased in 2000 and is a two-laser (488nm and 615nm), four-color, six-parameter, bench-top analyzer used primarily for evaluating surface and intracellular antigen expression, apoptosis detection, bead-based cytokine detection and cell-cycle analysis. Users are able to run their own samples on this instrument after training with the facility director.

 

The FACS Aria SOS is a fully-digital, four-laser (405nm, 488nm, 561nm and 640nm), ten-color, twelve-parameter instrument. It was purchased in 2010 by the Cancer Center. It is used for all cell sorting experiments (up to four populations simultaneously) and for immunophenotypic analysis requiring more than four colors. It is also equipped with an aerosol management option and temperature control.

 Other Services
  • Help with protocol and assay development and implementation, including selection of antibodies and fluorochromes.
  • Use of proper staining and compensation controls.
  • Selection of appropriate buffers and data analysis.
  • For investigators new to flow cytometry, the facility will also help purchase reagents and will assist in all aspects of sample preparation including cell staining, data acquisition and data analysis.
 Resource Director

Calvin Williams, MD, PhD

Dr. Williams joined MCW in 2002 and served as the Director of the CRI Flow Cytometry Core since its inception in 2005. Dr. Williams obtained his MD and PhD degrees from the University of California-Irvine in 1991, and has been active in research focused on the mechanisms of immunologic tolerance. Dr. Williams’ recent work involves the characterization of regulatory T cell development and function in models of inflammatory autoimmune disease. He also serves as the Chief Scientific Officer for the CRI and as an Associate Dean for Research at MCW.

 

 Key Technical Staff

Tamara Nelson, MS, Research Associate

Research Associate returned to MCW in 2013. She earned her graduate degree from
the University of Wisconsin-Madison Biotechnology in 2007. With over ten years of research experience in both academic and biotech industry, she is well versed in many molecular biology and immunochemical techniques. Ms. Nelson provides training for new users, schedules and executes cell sorting, maintains and calibrates the instruments, and manages data archiving for the shared facility. She has completed coursework at BD Biosciences to help troubleshoot sorting rare cell types, small and large cells, as well as maximizing sort optimization and cell recovery.
 Recent Project Examples
  • Identification of circulating endothelial progenitor cells in patients with infantile hemangioma – Drs. Beth Drolet and Kristen Holland, MCW Department of Pediatrics/Dermatology
     
  • CD45 isoform expression on CLL cells – Dr. Ali Bseiso, Marshfield Clinic
     
  • Microarray analysis of lymphocytes following culture with serum from CF patients – Dr. Hara Levy MCW Department of Pediatrics/Pulmonology

Cancer Center Shared Resources

Cores and Campus Shared Resources

Medical College of Wisconsin
8701 Watertown Plank Road
Milwaukee, WI 53226
(414) 955-8296
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© 2015

Page Updated 04/28/2015
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