Andrew Greene, PhD

Professor and Director, Biotechnology and Biomedical Engineering Center

Research areas:

Cardiovascular Physiology

Computational Biology & Bioinformatics

Molecular & Cellular Physiology

In a broad sense, our laboratory is attempting to understand fundamental principles of cardiovascular regulation and disease. Our research is highly collaborative and spans the areas of molecular and cellular biology, genetics, proteomics, technology development and microcirculatory experiments. Studies have focused on mechanisms of blood vessel growth and regression as well as the control of blood flow to tissues such as skeletal muscle and brain. We measure gene and protein expression, receptor numbers, oxygen transport, local blood flow, and microvascular density to assess the ability of the cardiovascular microcirculatory network to meet the needs of the tissue.

We perform in vivo experiments using Positron Emission Tomography, Magnetic Resonance Imaging and spectroscopy to measure changes in blood flow and metabolism in patients and animal models of disease as an aid to understanding basic mechanisms. Unique animal models produced with the aid of genomic manipulation are used in these experiments in an attempt to connect genes with cardiovascular functions.

Our lab data is analyzed with the aid of mathematical and computer models that we have developed based on morphological and experimental data. These models have proven to be extremely valuable in understanding the complex signals that cause changes in the circulation in response to normal and disease processes.

Recent Publications:

• Hicks WA, Halligan BD, Slyper RY, Twigger SN, Greene AS, Olivier M. Simultaneous Quantification and Identification Using (18)O Labeling with an Ion Trap Mass Spectrometer and the Analysis Software Application "ZoomQuant." J Am Soc Mass Spectrom. 2005 Jun;16(6):916-25.
• Halligan BD, Slyper RY, Twigger SN, Hicks W, Olivier M, Greene AS. ZoomQuant: an application for the quantitation of stable isotope labeled peptides. J Am Soc Mass Spectrom. 2005 Mar;16(3):302-6.
• Drenjancevic-Peric I, Greene AS, Kunert MP, Lombard JH. Arteriolar responses to vasodilator stimuli and elevated P(O2) in renin congenic and Dahl salt-sensitive rats. Microcirculation. 2004 Dec;11(8):669-77.
• Liang M, Cowley AW, Greene AS. High throughput gene expression profiling: a molecular approach to integrative physiology. J Physiol. 2004 Jan 1;554(Pt 1):22-30.
• Santos CF, Caprio MA, Oliveira EB, Salgado MC, Schippers DN, Munzenmaier DH, Greene AS. Functional role, cellular source, and tissue distribution of rat elastase-2, an angiotensin II-forming enzyme. Am J Physiol Heart Circ Physiol. 2003 Aug;285(2):H775-83.
• Amaral SL, Maier KG, Schippers DN, Roman RJ, Greene AS. CYP4A metabolites of arachidonic acid and VEGF are mediators of skeletal muscle angiogenesis. Am J Physiol Heart Circ Physiol. 2003 May;284(5):H1528-35.

Phone: (414) 456-8532 Lab website: bbc.mcw.edu Email: agreene@mcw.edu B.S. Biomedical & Electrical Engineering, Syracuse University, 1980 Ph.D. Biomedical Engineering, Johns Hopkins University, 1985