Cell Biology, Neurobiology & Anatomy

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Allison D. Ebert, PhD

Assistant Professor

Department of Cell Biology, Neurobiology & Anatomy

 

PhD, Northwestern University, Chicago, 2005
BS, Indiana University, Bloomington, 1999

Mailing Address
Department of Cell Biology, Neurobiology & Anatomy
8701 Watertown Plank Road
Milwaukee, WI 53226-3548
USA

Phone: (414) 955-2979
FAX: (414) 955-6517

Email: aebert@mcw.edu

List of publications

 

 

 

 


Research Area: Parkinson’s Disease, Huntington’s Disease, Spinal Muscular Atrophy, therapeutic uses of stem cells and disease modeling


neurons differentiated from
human embryonic stem cells

 

My research interests are in the area of neurodegenerative diseases, both understanding the molecular basis   for the disease progression and finding effective experimental therapies. My current research focuses on using induced pluripotent stem cells (iPSCs) derived from patient tissue to understand disease mechanisms and therapeutic intervention. Specifically, my lab is investigating the cell death processes involved in motor neuron loss in spinal muscular atrophy (SMA). We are using iPSCs derived from an SMA patient to generate motor neurons, astrocytes, and muscle cells to determine how the motor neurons are dying and whether the astrocytes and muscle cells are contributing to the disease process. My lab is also testing the therapeutic potential of ex vivo gene therapy in animal models of Parkinson’s and Huntington’s diseases by transplanting neural stem cells engineered to produce specific growth factors known to aid neuron survival. The growth factors I am actively examining are glial cell line-derivedneurotrophic factor (GDNF), insulin like growth factor (IGF-1), brain derived neurotrophic factor (BDNF), and vascular endothelial growth factor (VEGF). Finally, we are interested in characterizing and understanding survival, migration, and differentiation patterns of transplanted stem cells in models of disease, which could have important clinical relevance.


Selected Publications

The Huntington's Disease iPSC Consortium. Induced pluripotent stem cells from patients with Huntington's disease show CAG repeat associated phenotypes. Cell Stem Cell. 2012 (in press).

D. Sareen*, A.D. Ebert*, B.M. Heins, J. V. McGivern, L. Ornelas, C.N. Svendsen. Inhibition of apoptosis blocks human motor neuron cell death in a stem cell model of spinal muscular atrophy. PLoS One 7(6):e39113. 2012. *equal contributions

A.D. Ebert, A.E. Barber, B.M. Heins, C.N. Svendsen. Ex vivo delivery of GDNF maintains motor function and prevents neuronal loss in a transgenic mouse model of Huntington's disease. Exp Neurol 224:155-162. 2010.

A.D. Ebert, J. Yu, F.F. Rose, Jr., V.B. Mattis, C.L. Lorson, J.A. Thomson, C.N. Svendsen.  Induced pluripotent stem cells from a spinal muscular atrophy patient. Nature 457:277-280. 2009.

A.D. Ebert, A.J. Beres, A.E. Barber, C.N. Svendsen.  Human neural progenitor cells over-expressing IGF-1 protect dopamine neurons and restore function in a rat model of Parkinson's disease.  Exp Neurol 209:213-223. 2008.

A.D. Ebert, J. Yu, F.F. Rose, Jr., V.B. Mattis, C.L. Lorson, J.A. Thomson, C.N. Svendsen. Induced pluripotent stem cells from a spinal muscular atrophy patient. Nature. DOI: 10.1038/nature07677. Epub Dec. 21, 2008.

A.D. Ebert, E.L. McMillan, C.N. Svendsen. Isolating, expanding, and infecting human and rodent fetal neural progenitor cells. Curr Protoc Stem Cell Biol, Chapter 2:Unit 2D.2. 2008.

S. Behrstock*, A.D. Ebert*, S. Klein, M. Schmitt, J.M. Moore, C.N. Svendsen. Lesion-induced increase in survival and migration of human neural progenitor cells releasing GDNF. Cell Trans 17(7):753-62. 2008. *equal contributions

M.E. Emborg, A.D. Ebert, J. Moirano, S. Peng, M. Suzuki, E. Capowski, V. Joers, B.Z. Roitberg, P. Aebischer, C.N. Svendsen. GDNF-secreting human neural progenitor cells increase tyrosine hydroxylase and VMAT2 expression in MPTP-treated cynomolgus monkeys. Cell Trans 17(4):383-95. 2008.

A.D. Ebert, A.J. Beres, A.E. Barber, C.N. Svendsen. Human neural progenitor cells over-expressing IGF-1 protect dopamine neurons and restore function in a rat model of Parkinson’s disease. Exp Neurol 209(1):213-223. 2008.

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