Protein and Nucleic Acid Facility

BIAcore 3000 Instrument

The BIAcore 3000 instrument integrates surface plasmon resonance (SPR) technology with a microfluidics system to monitor molecular interactions in real time at concentrations ranging from pM to mM.  This label-free technology can detect a wide range of molecular masses from 180Da to >1000kDa.  The high sensitivity and high through-put capabilities allows for the detection of drug-protein, hormone-protein, protein-protein, DNA-protein, carbohydrate-protein, and lipid-protein interactions.  The ability to interface with mass spectrometers provides discovery-based research in proteomic studies. 

For more information about SPR technology, theory, and applications, please see the attached BIAcore presentation.

The BIAcore 3000 instrument is housed in the Department of Biochemistry and is available to all Medical College of Wisconsin faculty and staff who have been trained and demonstrate the ability to use microfluidic-based instrumentation. Training and consultation are available on an appointment basis.

For more information contact:

Nancy Dahms, Ph.D.
Medical College of Wisconsin
Department of Biochemistry
8701 Watertown Plank Road
Milwaukee, WI  53226
(414) 456-4698
ndahms@mcw.edu

Fees

Note:  A training period of no less than 4 hours is required before you can work unassisted on the instrument.  BIAcore chips and special reagents are not included in the fee.