This photomicrograph is a series of images of intestinal epithelial cells that have been stained so that we can visualize the expression levels of the adherens junction protein E-cadherin. To investigate the role for E-cadherin to participate in epithelial restitution, that is migration to cover an epithelial injury, we created cells in which E-cadherin had been knocked-down using shRNA technology. E-cadherin is labeled in green as shown in the wild-type cells. The actin cytoskeleton is shown stained with a marker that appears red using the fluorescence microscope. The decrease in green cooler in two separate cell lines, labeled sh1 and sh2, indicate that we have significantly depleted the E-cadherin protein in those cells.
shScramble and puromycin cells are included as two shRNA controls. These data were generated by Soony Hwang as part of his doctoral research.