Microbiology and Molecular Genetics

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William T. Jackson, PhD

Assistant Professor
Microbiology and Molecular Genetics
Medical College of Wisconsin

Research Focus: Viral Genetics, Virus-Host Cell Interactions

PhD: University of California, Berkeley (1999) Molecular and Cell Biology

 

My lab studies how viruses interact with their host cells, and we go about this by using the genetic diversity of one viral family as a tool. These studies utilize the family Picornaviridae, which encompasses viruses as diverse as Poliovirus, the causative agent of poliomyelitis; Rhinovirus, a major agent of the common cold; Hepatitis A virus, which causes acute liver disease; and Foot and Mouth Disease Virus, an economically devastating disease of livestock. Despite such an incredible diversity of disease, cellular effects, and viral host, these viruses are remarkably similar in genetic organization and sequence.

This begs the question of how relatively subtle genetic differences among viruses can lead to enormous differences in the viruses' interactions with cells and the outcome of infections. Using Poliovirus and Rhinovirus as starting points, our lab analyzes important phenotypic differences between viruses in the same family or species. In particular, we are interested in viral subversion of innate immune pathways such as autophagy, viral mechanisms to prevent immune surveillance such as inhibition of cell secretion, cell-to-cell viral transmission pathways, and intracellular virus replication in general.

In addition, we are using yeast as a model cell in which we can express viral proteins and observe effects on the cell with similarities to effects on mammalian cells. We also use clonally differentiated cell lines with different infection phenotypes to determine which host changes can alter viral growth. These approaches allow for simple "host genetic" analysis of the genetic pathways being altered by viral products. The goal of this research is ultimately an understanding of how subtle genetic differences in viruses can lead to evasion and subversion of the immune response, development of viral drug resistance, and evolution into new species.

Cellular effects of picornavirus.

A.  Arrows show unusual double-membraned structures in rhinovirus 14 infection.  Arrowheads designate virions in and near the structures.

B, C, D.  During poliovirus infection, unusual structures near the cell surface label with the autophagy maker protein GFP-LC3 and PV capsid protein.

 

Cellular effects of picornavirus.

A.  The autophagy marker GFP-LC3 and the protein LAMP-1 co-localize in response to autophagic induction by Rapamycin or Tamoxifen treatment.

B.  During poliovirus infection, GFP-LC3 and the protein LAMP-1 co-localize in a manner reminiscent of autophagy.

C.  These effects can be replicated using two different rhinovirus species.

 

Recent Publications

 

Contact Information:

Dr. William Jackson
Department of Microbiology and Molecular Genetics
Medical College of Wisconsin
Room 204 - BSB (Basic Science Building)
8701 Watertown Plank Road
Milwaukee, WI  53226


  
wjackson@mcw.edu
  
414-955-8456
414-955-6535 (Fax)

 

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Page Updated 03/13/2012