Redox and Bioenergetics Shared Resource (RBSR)

The Redox and Bioenergetics Shared Resource (RBSR) provides specialized instrumentation and techniques dedicated to investigate cancer cell metabolism and redox signaling for cancer center members. The RBSR is also an environment for education and training in research on oxy-radicals and redox bioenergetics. RBSR staff has expertise in designing and interpreting experiments in several cancer models, including breast, lung, pancreas, prostate, and skin. The RBSR offers services and instrumentation to assess many aspects of redox signaling and metabolic function in cancer cells. 


Balaraman Kalyanaraman, PhD | Resource Director | (414) 955-4059
Charles Myers, PhD | Redox Biology Lead | (414) 955-8593
Jacek Zielonka, PhD | Research Scientist | (414) 955-4789
Steve Komas | Research Technologist | (414) 955-4059

Location and Hours of Operation

Redox and Bioenergetics Shared Resource (RBSR)

The RBSR is located on the 2nd floor of the MACC Fund Research Center (MFRC). Standard hours of operation are from 9 a.m. to 5 p.m. Off-hour access to equipment is available in support of experiments requiring unique schedules. To schedule an appointment for experiment design services and to use the RBSR equipment, please contact Steve Komas at (414) 955-4789.

Services and Equipment

A wide range of experimental applications of extracellular flux, metabolomics and redox signaling studies may be performed with the instruments and expertise in the RBSR. The RBSR facility supports cancer investigators by examining mitochondrial function, glycolytic function, fatty acid oxidation, glucose metabolism, glutamine metabolism and metabolic phenotypes in cancer models. The RBSR offers enhanced and rapid detection and quantification of extra- and intra-cellular substrates and metabolites, metabolic profiling /phenotyping, reactive oxygen species (ROS) detection, redox signaling, and drug/compound screening.

Services include: Major equipment in the RBSR:

Bioenergetic flux analyses

LCMS-based analyses

HPLC analyses

HPLC-ECD analyses

Plate reader-based analyses

Compound synthesis

Data analysis/Consultation

Redox immunoblotting

  Seahorse Bioscience XF24 and XF96 Extracellular Flux Analyzers

  ESA HPLC system with electrochemical CoulArray® detector

  Shimadzu Nexera-2 UHPLC system with absorption and MS/MS detectors

  Beckman Coulter DTX 880 Multimode Plate Reader

  Agilent 1100 HPLC systems with absorption and fluorescence detectors

  Nikon Eclipse Ti Fluorescence Microscope

  Bruker EMX Electron Paramagnetic Resonance (EPR) spectrometer

  Perkin Elmer LS55 luminescence spectrometer