Seahorse Bioscience Extracellular Flux Analyzer
The Seahorse XF-96 measures the rate of change of analytes dissolved oxygen and pH in media immediately surrounding living cells cultured in a microplate. Changes in the extracellular media are caused by the consumption or production of analytes by the cells. Therefore, a sensitive measurement of the media flux can be used to determine rates of cellular metabolism with great precision and in a totally noninvasive, label free manner. The analyzer can make measurements in as little as five minutes. Cells can be profiled over a period of minutes, hours, or days.
The theory behind this instrument utilizes the fact that normal cells rely on mitochondrial oxidative phosphorylation (OXPHOS) to produce ATP (34 molecules of ATP/one molecule of glucose) as a source of cellular energy. On the other hand, most cancer cells utilize aerobic glycolysis which is energetically inefficient, to generate ATP (2 ATP/one molecule of glucose) with a decreased use of TCA cycle. Subsequently, tumor cells are glycolytic and actively take up much more glucose. Glucose is converted to G-6-P by high intracellular levels of hexokinases. This metabolic shift from OXPHOS to aerobic glycolysis with increased lactate production is known as the "Warburg effect."
- The cost per experimental plate is $200.
- This price includes the compounds utilized in a basic mitochondrial stress test (oligomycin, FCCP, and ANT.A/Rotenone)
Certain stressors can have different effects on oxygen consumption in various cell lines. Examples of oxidative stressor can be seen below:
Figure from Dranka et. al FRBM (2011, in press)
The Seahorse XF-96 has the ability to analyze both OCR and ECAR simultaneously without disrupting the cells.
If you would like to schedule a time to use the XF-96, please call Steve Komas at (414) 955-4059 or email email@example.com.
View the XF-96 Google Calendar to find out if the XF-96 is available to use at your desired time.