The major mission of our laboratory is to understand mechanisms regulating the cerebral circulation under physiologic and pathophysiologic conditions. Our research interests involve identification and characterization of different ion channel types and different second messenger systems in cerebral vascular smooth muscle cells, endothelial cells, neurons and in glial cells or astrocytes. Studies in the laboratory mainly focus on examining the interaction between ion channels and lipid mediators, second messengers, hypoxia, free radicals as well as different enzymatic pathways that could evoke compensatory changes in calcium homeostasis, neuronal activity and cerebral blood flow.
We are also investigating the influence of pathophysiological states such as ischemia reperfusion, insulin-dependent diabetes mellitus and Alzheimer's disease on ion channel function, cerebral vascular reactivity and second messenger systems for possible elucidation of their influence on normal cerebral vascular reactivity and autoregulation of cerebral blood flow. Laser-Doppler flowmetry is used in healthy and diseased subjects to unravel and understand if alterations in ion channel function and endogenous signaling mechanisms negatively or positively influence distribution and autoregulation of cerebral blood flow. Our research areas include patch clamp electrophysiological screening of different ion channel current types including L-type Ca2+ channels, different types of K+channels, chloride channels, and a variety of TRP channels in isolated cerebral and coronary vascular smooth muscle cells, endothelial cells neurons and astrocytes; measurement of laser Doppler cerebral blood flow using, biochemical assay of CYP 450-derived lipid metabolites, reactive oxygen species, protein kinases and protein phosphatases, molecular analysis of ion channels, secreted proteins and physiology of adenosine receptors in different brain cell types.