Medical College of Wisconsin
Medical College of Wisconsin Medical College of Wisconsin
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Jianhua Fu, PhD

Jianhua Fu

Jianhua Fu, PhD

Associate Professor

Locations

  • Biochemistry
    BSB 303

Contact Information

Education

PhD, University of Pittsburgh, 1994

Biography

Dr. Fu received his PhD degree in Protein Crystallography from University of Pittsburgh in 1994. He spent a postdoctoral period in the laboratory of Roger Kornberg at Stanford University School of Medicine, where he cracked the phase problem in determining the crystallographic structure of RNA Polymerase II. Dr. Fu received fellowship awards from Universitywide AIDS Research Program (University of California) and American Cancer Society. Since 2000, his laboratory has been funded by the NIH to study the structural mechanism of RNA polymerase II machinery. Dr. Fu joined the faculty at Medical College of Wisconsin in 2007. Dr. Fu welcomes MCW graduate students to discuss prospects of thesis research in the area of Structural Biology. Potential postdoctoral scientists are encouraged to communicate their interests as well.

Research Experience

  • Biochemistry
  • Biophysics

Methodologies and Techniques

  • X-ray Crystallography; Biochemistry

MCW Program / Core Facilities

  • Structural Biology; Protein Crystallization; X-ray Crystallography

Educational Expertise

  • Structural Biology; Biochemistry

Research Interests

Structure of the 12-subunit RNA polymerase II refined with the aid of anomalous diffraction data.

This figure shows the most complete structure of RNA Polymerase II (Pol II), the enzyme that reads genetic sequences from chromosomes in the nucleus of a cell. The Pol II model is rendered in the form of ribbons (most of it in blue) and laid over a background image of chromosomal DNA. The structural work was performed in the Fu lab by utilizing the power of anomalous scattering from 8 Zn2+ ions bound intrinsically in the polymerase. This latest model reveals the previously undetermined loop (green) that is implicated in contacting the general transcription factor TFIIF, and defines conformation for the loop (orange) that crosslinks with TFIIE. Fu Image 1Several functionally significant elements are highlighted as follows: yellow, Fork Loop-1; grey-brown, Fork Loop-2; pink, Rudder and red (sphere), the catalytic Mg2+ site. The chromosomal DNA is depicted for its unraveling at the different levels, from the sister chromatids to 30-nm chromatin fiber and 10-nm nucleosomal array.

This work is described in: Meyer PA, Ye P, Suh MH, Zhang M, Fu J., J Biol Chem. 2009 Mar 16. [Epub ahead of print]

Publications

Publications of Jianhua Fu PhD from the Faculty Collaboration Database