Dr. Fu received his PhD degree in Protein Crystallography from University of Pittsburgh in 1994. He spent a postdoctoral period in the laboratory of Roger Kornberg at Stanford University School of Medicine, where he cracked the phase problem in determining the crystallographic structure of RNA Polymerase II. Dr. Fu received fellowship awards from Universitywide AIDS Research Program (University of California) and American Cancer Society. Since 2000, his laboratory has been funded by the NIH to study the structural mechanism of RNA polymerase II machinery. Dr. Fu joined the faculty at Medical College of Wisconsin in 2007. Dr. Fu welcomes MCW graduate students to discuss prospects of thesis research in the area of Structural Biology. Potential postdoctoral scientists are encouraged to communicate their interests as well.
Methodologies and Techniques
- X-ray Crystallography; Biochemistry
MCW Program / Core Facilities
- Structural Biology; Protein Crystallization; X-ray Crystallography
- Structural Biology; Biochemistry
Structure of the 12-subunit RNA polymerase II refined with the aid of anomalous diffraction data.
This figure shows the most complete structure of RNA Polymerase II (Pol II), the enzyme that reads genetic sequences from chromosomes in the nucleus of a cell. The Pol II model is rendered in the form of ribbons (most of it in blue) and laid over a background image of chromosomal DNA. The structural work was performed in the Fu lab by utilizing the power of anomalous scattering from 8 Zn2+ ions bound intrinsically in the polymerase. This latest model reveals the previously undetermined loop (green) that is implicated in contacting the general transcription factor TFIIF, and defines conformation for the loop (orange) that crosslinks with TFIIE. Several functionally significant elements are highlighted as follows: yellow, Fork Loop-1; grey-brown, Fork Loop-2; pink, Rudder and red (sphere), the catalytic Mg2+ site. The chromosomal DNA is depicted for its unraveling at the different levels, from the sister chromatids to 30-nm chromatin fiber and 10-nm nucleosomal array.
This work is described in: Meyer PA, Ye P, Suh MH, Zhang M, Fu J., J Biol Chem. 2009 Mar 16. [Epub ahead of print]
(Meyer PA, Li S, Zhang M, Yamada K, Takagi Y, Hartzog GA, Fu J.) Mol Cell Biol. 2015 Oct;35(19):3354-69 PMID: 26217010 PMCID: PMC4561723 SCOPUS ID: 2-s2.0-84941126330 07/29/2015
(Dutta A, Babbarwal V, Fu J, Brunke-Reese D, Libert DM, Willis J, Reese JC.) Mol Cell Biol. 2015 Jun 01;35(11):1915-25 PMID: 25776559 PMCID: PMC4420917 SCOPUS ID: 2-s2.0-84929414078 03/18/2015
(Babbarwal V, Fu J, Reese JC.) J Biol Chem. 2014 Nov 28;289(48):33125-30 PMID: 25315781 PMCID: PMC4246073 SCOPUS ID: 2-s2.0-84912098443 10/16/2014
(Hartzog GA, Fu J.) Biochim Biophys Acta. 2013 Jan;1829(1):105-15 PMID: 22982195 PMCID: PMC3545043 SCOPUS ID: 2-s2.0-84872424651 09/18/2012
(Meyer PA, Fu J.) Structure. 2012 May 09;20(5):755-7 PMID: 22579244 PMCID: PMC3351759 05/15/2012
(Kruk JA, Dutta A, Fu J, Gilmour DS, Reese JC.) Genes Dev. 2011 Mar 15;25(6):581-93 PMID: 21406554 PMCID: PMC3059832 SCOPUS ID: 2-s2.0-79952609007 03/17/2011
(Suh MH, Meyer PA, Gu M, Ye P, Zhang M, Kaplan CD, Lima CD, Fu J.) J Biol Chem. 2010 Oct 29;285(44):34027-38 PMID: 20720002 PMCID: PMC2962502 SCOPUS ID: 2-s2.0-77958608570 08/20/2010
(Chang WH, Chiu MT, Chen CY, Yen CF, Lin YC, Weng YP, Chang JC, Wu YM, Cheng H, Fu J, Tu IP.) Structure. 2010 Jan 13;18(1):17-27 PMID: 20152149 02/16/2010
(Meyer PA, Ye P, Suh MH, Zhang M, Fu J.) J Biol Chem. 2009 May 08;284(19):12933-9 PMID: 19289466 PMCID: PMC2676024 03/18/2009
(Meyer PA, Ye P, Zhang M, Suh MH, Fu J.) Structure. 2006 Jun;14(6):973-82 PMID: 16765890 06/13/2006
(Suh MH, Ye P, Zhang M, Hausmann S, Shuman S, Gnatt AL, Fu J.) Proc Natl Acad Sci U S A. 2005 Nov 29;102(48):17314-9 PMID: 16301539 PMCID: PMC1297677 11/23/2005
(Zhang Z, Fu J, Gilmour DS.) Genes Dev. 2005 Jul 01;19(13):1572-80 PMID: 15998810 PMCID: PMC1172063 07/07/2005