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Orlando Landon DeLeon

Orlando DeLeon_Academic Profile

Orlando "Landon" DeLeon

Graduate Student

Locations

  • Microbiology & Immunology

Contact Information

Biography

Mentor: John Kirby, PhD
Year Entered MCW: 2017
Previous Education: BA, Northwestern University, Evanston, IL, 2010

Research Interests

We have been examining the role of xenobiotics for their capacity to disrupt the gut microbiota with deleterious consequences on metabolism. Currently, we utilize the Illumina platform to obtain 16s rDNA sequence information and metagenomic (whole genome) information. We analyze those data using QIIME (Quantitative Insights into Microbial Ecology) and ANVI’O (An analysis and visualization platform for ‘omics data) open source software. We are employing the use of total calorimetry to assess metabolic defects in mice following perturbation with xenobiotics.

“My thesis is on the xenobiotic risperidone and its ability to alter both the bacterial and viral components of the microbiome. We use this drug to model how xenobiotics can have adverse consequences, including dysbiosis, or an ‘unhealthy’ microbiome. I examine how phages (bacterial viruses) can mediate community changes in the gut, both in the gene content and composition of the microbiome. Much of my work focuses on de novo viral genome assemblies (virus hunting), bacterial genome assemblies, and network analysis of gene pathways, all of which are applied to a community-based perspective. I also work on developing in vitro models to study this phage-bacterial dynamic shift due to risperidone treatment. When not in the lab, I’m likely eating sushi or ramen.”