Research Lab Bench
Natalia Stein

Natalia Stein, PhD

Postdoctoral Fellow (Subczynski Lab)

Faculty Advisor

  • W. Karol Subczynski

Locations

  • Biophysics
    MFRC 2010

Contact Information

Education

Postdoctoral Fellow, Marquette University, Milwaukee, WI, 2014-2016
PhD, Biochemistry, University of Wisconsin-Milwaukee, Milwaukee, WI, 2014
BS, Biochemistry (Minor, Biology; Certificate, Biotechnology), University of Wisconsin-Milwaukee, Milwaukee, WI, 2007

Research Interests

Although I come from the world of enzymology, I am currently working on membrane organization, specifically describing the phospholipid organization in the eye membranes. organization in the eye membranes.

Publications

  1. Youngblut M, Pauly DJ, Stein N, Walters D, Conrad JA, Moran GR, Bennett B, Pacheco AA. (2014). Shewanella oneidensis Cytochrome c Nitrite Reductase (ccNiR) does not disproportionate hydroxylamine to ammonia and nitrite, despite a strongly favorable driving force. Biochemistry, 53(13), 2136-2144. doi: 10.1021/bi401705d
  2. Stein N, Love D, Judd E, Elliott SJ, Bennett B, Pacheco AA. (2015). Correlations between the electronic properties of Shewanella  oneidensis cytochrome c nitrite reductase (ccNiR) and its structure: effects of heme  oxidation state and active site ligation. Biochemistry, 54(24), 3749-3758, DOI: 10.1021/acs.biochem.5b00330
  3. Stein N, Gumataotao N, Hajnas N, Wu R, Wasantha Lankathilaka KP, Bornscheuer UT, Liu D, Fiedler AT, Holz RC, Bennett B. (2017). Multiple States of Nitrile Hydratase from Rhodococcus equi TG328-2: Structural and Mechanistic Insights from EPR and DFT Studies. Biochemistry, 56(24), 3068-3077, 10.1021/acs.biochem.6b00876
  4. Lankathilaka KPW, Stein N, Holz RC, Bennett B. Cellular maturation of an iron-type nitrile hydratase interrogated using EPR spectroscopy. J Biol Inorg Chem. 2019 Oct;24(7):1105-1113. doi: 10.1007/s00775-019-01720-y.
  5. Stein N, Mainali L, Hyde JS, Subczynski WK. Characterization of the distribution of spin-lattice relaxation rates of lipid spin labels in fiber cell plasma membranes of eye lenses with a stretched-exponential function. Appl Magn Reson. 2019 Jul;50(7):903-918. doi: 10.1007/s00723-019-01119-7.
  6. Ali M, Stein N, Mao Y, Shahid S, Schmidt M, Bennett B, Pacheco AA. Trapping of a Putative Intermediate in the Cytochrome c Nitrite Reductase (ccNiR)-Catalyzed Reduction of Nitrite: Implications for the ccNiR Reaction Mechanism. J Am Chem Soc. 2019 Aug 28;141(34):13358-13371.
  7. Stein N, Subczynski K. Oxygen transport parameter in plasma membrane of eye lens fiber cells by saturation recovery EPR. Appl Magn Reson. Appl. Magn. Reson. 52:61-80, 2021.
  8. Stein N, Subczynski WK. Differences in the properties of porcine cortical and nuclear fiber cell plasma membranes revealed by saturation recovery EPR spin labeling measurements. Exp Eye Res. 2021 Mar 11:108536. doi: 10.1016/j.exer.2021.108536.
  9. Subczynski WK, Pasenkiewicz-Gierula M, Widomska J, Stein N. Role of cholesterol in maintaining the physical properties of the plasma membrane. In: Cholesterol: from Biophysics to the Clinics, AN Bukiya and AM Dopico Eds, Elsevier, 2021.