The Medical College of Wisconsin Cancer Center Flow Cytometry (FLOW) facility offers state-of-the art technology with a wide range of instrumentation at two sites - the Medical College of Wisconsin and Versiti Blood Research Institute. The directors at each site have more than 40 years’ combined experience. This ensures a high degree of quality control and efficiency. The shared resource consistently provides onsite, cost-effective, comprehensive analytic flow cytometry and high-speed cell sorting services and expertise.
The Medical College of Wisconsin site has one LSRII and the Blood Research Institute operation has two LSRII analytical flow cytometers. All instruments have similar laser configurations.
The Blood Research Institute has one FACSCelesta cytometer with 4 lasers capable of detecting 17 colors.
The Medical College of Wisconsin site has a two-laser (488nm, 615 nm), four-color, six-parameter, bench-top analyzer used primarily for evaluating surface and intracellular antigen expression, apoptosis detection, bead-based cytokine detection and cell-cycle analysis.
BD Accuri C6
This is a two-laser (488nm, 640 nm) personal flow cytometer located at the Blood Research Institute. It is the newest generation of the BD Accuri platform. The BD Accuri C6 supports a wide array of applications including immunology, as well as cell and cancer biology. Users are charged to collect data on the BD Accuri.
FACSAria II Special Order Systems (SOS)
FLOW has three FACSAria II cell sorting systems, two at the Medical College of Wisconsin and one at the Blood Research Institute.