Pathology Research BioCore (PRB)
The Pathology Research BioCore (PRB) Shared Resource at the Medical College of Wisconsin Cancer Center is a valuable resource for all aspects of experimental pathology for MCWCC members conducting basic, translational, clinical and population science research on human cancer. The PRB supports multiple aspects of cancer research by 1) utilizing universal consenting for all MCWCC patients making tissues easily available for subsequent analysis, 2) offering its members competitive pricing on services including centralized banking and distribution of cancer tissues and blood, and 3) providing sophisticated tissue-arraying services and comprehensive histological and molecular analysis of cancer.
Location and Hours of Operation
The PRB is open M-F from 8:00 am-5:00 pm and is located in the Wisconsin Diagnostic Laboratory building.
Services and Equipment
The PRB analytics laboratory is a 5,000-square-foot, CLIA- and CAP-accredited facility. The current staffing of the PRB analytics laboratory includes nine full-time employees. Equipment includes three automated DAKO immunohistochemistry strainers (one Autostainer Plus, one Link48, and one Omnis), four high-capacity sequencing machines (two Ion Torrent PGMs and two Illumina miniSeqs), a Perkin-Elmer/3DHistech Panoramic 250 Flash bright field and fluorescence scanner, digital image analysis systems (Definiens Tissue Studio and Developer and DAKO ACIS III), Perkin-Elmer/3DHistech TMA Grandmaster high throughput automated tissue arrayer, a Veridiam VTA-110C basic tissue arrayer, a fluorescence microscope, several dedicated servers for data analysis, and storage capacity of nearly 1.5 Petabytes for big sequencing and image data files.
The PRB strives to provide the following services to MCW Cancer Center members:
Procure and distribute biospecimens from consented patients and de-identified annotated data to investigators through a streamlined, simple and compliant process. This includes viable tissue for patient-derived xenograft (PDX) modeling in vivo and 3D organoid cultures or cell line generation in vitro and fresh frozen or formalin-fixed, paraffin-embedded (FFPE) tissues for molecular studies. In addition, the specimen database securely and effectively links clinical annotations with specimens.
Provide access to specialized analytical technologies, services and expertise in order to enhance scientific interaction and productivity. This includes nucleic acid sequencing and analyses, quantitative immunohistochemistry and histology image analyses and associated technical consultation.
Promote, design, perform, manage and interpret cancer-related research projects utilizing human cancer specimens at MCW that ensures stability, cost-effectiveness and quality control. This includes advanced tissue array generation services and tissue extraction services (nucleic acids and protein). Sample normalization and preparation for genotyping and expression studies are also provided.