Redox and Bioenergetics Shared Resource (RBSR)
The Redox and Bioenergetics Shared Resource (RBSR) provides specialized instrumentation and techniques to investigate cancer cell metabolism and redox signaling. The RBSR is also an environment for education and training in research on oxy-radicals and redox bioenergetics. RBSR staff has expertise in designing and interpreting experiments in several cancer models, including breast, lung, pancreas, prostate, and skin.
Services and Equipment
The RBSR facility supports cancer investigators by examining mitochondrial function, glycolytic function, fatty acid oxidation, glucose metabolism, glutamine metabolism and metabolic phenotypes in cancer models. We offer enhanced and rapid detection and quantification of extra- and intra-cellular substrates and metabolites, metabolic profiling/phenotyping, reactive oxygen species (ROS) detection, redox signaling, and drug/compound screening.
- Bioenergetic flux analyses
- LCMS-based analyses
- HPLC analyses
- HPLC-ECD analyses
- Plate reader-based analyses
- Compound synthesis
- Data analysis/Consultation
- Redox immunoblotting
Major equipment in the RBSR:
- Seahorse Bioscience XF24 and XF96 Extracellular Flux Analyzers
- ESA HPLC system with electrochemical CoulArray® detector
- Shimadzu Nexera-2 UHPLC system with absorption and MS/MS detectors
- Beckman Coulter DTX 880 Multimode Plate Reader
- Agilent 1100 HPLC systems with absorption and fluorescence detectors
- Nikon Eclipse Ti Fluorescence Microscope
- Bruker EMX Electron Paramagnetic Resonance (EPR) spectrometer
- Perkin Elmer LS55 luminescence spectrometer
Location and Hours of Operation
2nd floor, MACC Fund Research Center
M-F from 9 a.m.-5 p.m.
Off-hour access is available in support of experiments requiring unique schedules.