Jennifer Strande Lab

Strande Lab

Jennifer Strande

Jennifer Strande, MD, PhD
Principal Investigator
Associate Professor, Department of Medicine
jstrande@mcw.edu

We study heart disease at varying levels of biological complexity including patients, animal models and individual heart cells to obtain insights into disease mechanisms. We are currently investigating the cellular and molecular mechanisms that cause cardiomyopathy in patients with muscular dystrophy.

Melanie Gartz

Melanie Gartz, MS, MHS
Research Technologist II
mreiter@mcw.edu

Our laboratory is interested in understanding the mechanisms underlying cardiomyopathy, a leading cause of death in patients with Duchenne and Becker muscular dystrophy. Our model system to investigate this phenomenon uses iPS cells differentiated into cardiomyocytes that contain patient specific dystrophin mutations. My role in the laboratory is to maintain our iPS lines and differentiate them into cardiomyocytes for experimentation. Furthermore, I have been investigating the factors contributing to oxidative stress in dystrophic iPS derived cardiomyocytes in order to lead to greater understanding of this unique cardiomyopathy.

Leah Thomas

Leah Thomas
Research Technologist I
lethomas@mcw.edu

My primary role within the lab is to provide support for various projects throughout the lab. Currently, my focus is developing in vitro model systems to investigate dystrophic cardiomyopathy and cellular senescence. Techniques I use to support the model systems are maintenance of iPSCs and differentiating them into cardiac and endothelial cells, CRISPR Cas-9 gene editing, and animal behavior studies.

Muhammad Zeeshan Afzal

Muhammad Zeeshan Afzal, BPharm, PhD
Postdoctoral Fellow
mafzal@mcw.edu

Dystrophic cardiomyopathy manifested in muscular dystrophy patients is due to mutations of the dystrophin gene, resulting in defective cytoskeletal protein involved in regulation of stretch associated cellular support. My research involves establishing bio-bank of dystrophic patient samples; reprograming of dystrophic patient derived somatic cells into induced pluripotent stem (iPS) cells; and their differentiation into cardiomyocytes. My further investigations focus undertaking fundamental assays to elucidate molecular mechanisms regulating oxidative stress in dystrophic cardiomyocytes and to study their cellular bioenergetics. Our efforts would help establishing basis to understand the oxidative mechanisms contributing towards dystrophic cardiomyopathy.

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Alumni/Former Trainees

Chelsea Fujinaka

Cardiac Remodeling and Reversible Pulmonary Hypertension During Pneumonitis in Rats after 13-Gy Partial-Body Irradiation with Minimal Bone Marrow Sparing: Effect of Lisinopril.

(Jacobs ER, Narayanan J, Fish BL, Gao F, Harmann LM, Bergom C, Gasperetti T, Strande JL, Medhora M.) Health Phys. 2019 Jan 08.
Exosomes exert cardioprotection in dystrophin-deficient cardiomyocytes via ERK1/2-p38/MAPK signaling.

(Gartz M, Darlington A, Afzal MZ, Strande JL.) Sci Rep. 2018 Nov 08;8(1):16519.
Examining the Paracrine Effects of Exosomes in Cardiovascular Disease and Repair.

(Gartz M, Strande JL.) J Am Heart Assoc. 2018 Jun 01;7(11).
Epitope Mapping of SERCA2a Identifies an Antigenic Determinant That Induces Mainly Atrial Myocarditis in A/J Mice.

(Krishnan B, Massilamany C, Basavalingappa RH, Gangaplara A, Rajasekaran RA, Afzal MZ, Khalilzad-Sharghi V, Zhou Y, Riethoven JJ, Nandi SS, Mishra PK, Sobel RA, Strande JL, Steffen D, Reddy J.) J Immunol. 2018 01 15;200(2):523-537.
β1-Adrenergic Receptor Contains Multiple IA and IE Binding Epitopes That Induce T Cell Responses with Varying Degrees of Autoimmune Myocarditis in A/J Mice.

(Basavalingappa RH, Massilamany C, Krishnan B, Gangaplara A, Rajasekaran RA, Afzal MZ, Riethoven JJ, Strande JL, Steffen D, Reddy J.) Front Immunol. 2017;8:1567.
Bariatric surgery as a treatment for heart failure: review of the literature and potential mechanisms.

(Kindel TL, Strande JL.) Surg Obes Relat Dis. 2018 01;14(1):117-122.
Branched chain α-ketoacid dehydrogenase kinase 111-130, a T cell epitope that induces both autoimmune myocarditis and hepatitis in A/J mice.

(Krishnan B, Massilamany C, Basavalingappa RH, Gangaplara A, Kang G, Li Q, Uzal FA, Strande JL, Delhon GA, Riethoven JJ, Steffen D, Reddy J.) Immun Inflamm Dis. 2017 12;5(4):421-434.
Generation of human iPSCs from urine derived cells of a non-affected control subject.

(Afzal MZ, Gartz M, Klyachko EA, Khan SS, Shah SJ, Gupta S, Shapiro AD, Vaughan DE, Strande JL.) Stem Cell Res. 2017 01;18:33-36.
Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation.

(Afzal MZ, Gartz M, Klyachko EA, Khan SS, Shah SJ, Gupta S, Shapiro AD, Vaughan DE, Strande JL.) Stem Cell Res. 2017 01;18:41-44.
Early Weight Loss Independent Effects of Sleeve Gastrectomy on Diet-Induced Cardiac Dysfunction in Obese, Wistar Rats.

(Kindel TL, Foster T, Goldspink P, Kindel SJ, Corbett J, Widlanksy M, Strande J.) Obes Surg. 2017 09;27(9):2370-2377.
Systemic AAV8-Mediated Gene Therapy Drives Whole-Body Correction of Myotubular Myopathy in Dogs.

(Mack DL, Poulard K, Goddard MA, Latournerie V, Snyder JM, Grange RW, Elverman MR, Denard J, Veron P, Buscara L, Le Bec C, Hogrel JY, Brezovec AG, Meng H, Yang L, Liu F, O'Callaghan M, Gopal N, Kelly VE, Smith BK, Strande JL, Mavilio F, Beggs AH, Mingozzi F, Lawlor MW, Buj-Bello A, Childers MK.) Mol Ther. 2017 04 05;25(4):839-854.
Molecular Approaches in HFpEF: MicroRNAs and iPSC-Derived Cardiomyocytes.

(Kriegel AJ, Gartz M, Afzal MZ, de Lange WJ, Ralphe JC, Strande JL.) J Cardiovasc Transl Res. 2017 Jun;10(3):295-304.

Cardiovascular Center

Jennifer Strande Lab

Recent Publications