Physician Staff Collaborate Conf Room

Institutional Biosafety Committee (IBC)

Host Vector Systems

Transgene
The sequence of interest in the cloning procedure. One important characteristic of a transgene is its origin – what species it derives from originally.

Host
A temporary or endpoint organism used to replicate or express rDNA. For instance, E. coli K-12 is used to replicate plasmids, HEK 293 cells are used to replicate viral vectors, or transgenic organisms.

Vector
Supplemental DNA used to manipulate (deliver, express, replicate, recombine) the transgene. For instance, some plasmids and viruses have been engineered to manipulate transgenes.

Host-Vector-Transgene Systems (H-V-T)
A basic unit of the NIH rDNA Guidelines. Certain H-V-Ts have been exempted from the requirement for registration with the IBC such as E. coli K12, Saccharomyces and Bacillus subtilis. Many projects that involve rDNA require several H-V-Ts to manipulate the transgene into the environment where its effects can be studied in vivo. An important part of the IBC review process is for the IBC and PI to jointly determine what safety precautions and practices need to take place in the use of the H-V-Ts in the proposed project.

 

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