Talks & Posters
Talks and posters presented by EPR Center faculty and staff are listed.
Talks
Fifth Biennial Conference on Resting State and Brain Connectivity, Vienna, Austria | September 21-24, 2016
Hyde JS. Meta-metallic Surface Coil for Enhanced Resting-State Sensitivity in the Rat Cerebellum.
X International Workshop on EPR in Biology and Medicine, Krakow, Poland | October 2-6, 2016
Feix J. Membrane-Protein Interactions.
Hyde JS. Broadband W-band EPR spectroscopy.
Kalyanaraman B. Mitochondria-Targeted Metformin: Antiproliferative Agents.
Klug CS. Insights into the Mechanism of LPS Transport in E. coli Using Site-Directed Spin Labeling EPR Spectroscopy.
Subczynski WK. High Cholesterol/Low Cholesterol: Effects in Biological Membranes.
Vasquez Vivar J. Thiol-Based Regulation of eNOS Activity is Not Uncoupling.
Zielonka J. Novel Inhibitors of NOX Enzymes.
Posters
Biophysical Society 60th Annual Meeting, Los Angeles, CA | February 28–March 2, 2016
Mainali L, Raguz M, O'Brien WJ, Subczynski W. Properties and Organization of Lipids in Membranes Derived from the Total Lipids Extracted from the Clear Human Lens Cortex and Nucleus of Donors from Different Age Groups.
Mainali L, Zareba M, Subczynski W. Oxidation of Cholesterol and Formation of Cholesterol Hydroperoxides Decreases the Cholesterol Concentration at which Formation of Cholesterol Bilayer Domains and Cholesterol Crystals is Initiated in Phospholipid Bilayers.
Tiwari V, Schuh AM, Klug CS, Czajkowski C. Radial Tilting of the Extracellular Domain of GLIC Revealed by EPR Spectroscopy.
ARVO Imaging in the Eye Conference, Seattle, WA | May 1–5, 2016
Subczynski W, Mainali L, Raguz M, O'Brien WJ. Functions of the Lipid Bilayer Portion of the Fiber Cell Plasma Membranes in the Maintaining Lens Homeostasis
G-protein Coupled Signaling Workshop, Ithaca, NY | June 14, 2016
Chen Q, Gilbert NC, Perry NA, Zhuo Y, Berndt S, Vishnivetskiy SA, Tholen J, Klug CS, Gurevich VV, Iverson TI. Structural Basis for Arrestin-3 Activation and Signaling.
58th Rocky Mountain Conference on Magnetic Resonance / 39th International EPR Symposium, Breckenridge, CO | July 17-22, 2016
Johnson BJ, Antholine WE, Lindeman SV, Mankad NP. EPR for a Cu4S Model for Nitrous Oxide Reductase.
X International Workshop on EPR in Biology and Medicine, Krakow, Poland | October 2-6, 2016
Debowska K, Debski D, Smulik-Izydorczy R, Michalski R, Piotrowski L, Michalowski B, Zielonka J, Kalyanaraman B, Sikora A. What Do We Really Know about Boronates Reactivity?
Kolander K, Dai Q, Lai S, Myers C, Zielonka J, Kalyanaraman B, Benjamin IJ. The Redox Biology Paradigm: Past, Present, and Future.
Pasenkiewicz-Gierula M, Szczelina R, Milanovic B, Markiewicz M, Subczynski WK. Self-Organization of Cholesterol and Oxysterol in Water.
Podsiadly R, Siarkiewicz P, Zielonka J, Zielonka M, Michalski R, Kalyanaraman B. Boronate-Based Fluorogenic Probes for the In Vivo Imaging of Peroxynitrite.
Raguz M, Mainali L, O'Brien WJ, Subczynski WK. Eye Lense: Lipid-Protein Interactions.
Widomska J, Mainali L, Raguz M, Subczynski WK. Cholesterol Bilayer Domain in the Eye Lens Health.
Zadlo A, Szewczyk G, Sarna M, Sidabras J, Camenisch T, Ito S, Wakamatsu K, Sarna T. Photobleaching of a Synthetic Pheomelanin Modifies its Paramagnetic Properties and Photoreactivity.
Talks
EPR Methodologies Enabled by Loop-Gap Resonators, Milwaukee, WI | August 22-23, 2015
Feix JF. SDSL Studies of the Bacterial Phospholipase ExoU.
Hyde JS. New Non-Adiabatic Rapid Sweep (NARS) Spectroscopic EPR Methods.
Klug CS. Insights into the Mechanism of LPS Trasport in E. coli.
Sidabras JS. Non-Adiabatic Rapid Sweep: Transition to Incipient Passage, Quadrature Detection, and W-band.
Subczynski WK. Organization of Lipids in Model and Biological Membranes Using Saturation Recovery EPR at X-and W-bands.
Vasquez-Vivar. Improving Spin-Label Oximetry: Approaching Single Cell.
Posters
Biophysical Society 59th Annual Meeting, Baltimore, MD | February 2015
Mainali L, Sidabras JW, Camenisch TG , Vasquez-Vivar J, Hyde JS, Subczynski WK. Spin-Labeled Uni-Lamellar Vesicles as an Oxygen Sensitive Analyte for Measurement of Cellular Respiration using Rat Dopaminergic Neuronal Cells.
Petrou VI, Clarke OB, Schultz KM, Tomasek D, Kloss B, Banerjee S, Rajashankar KR, Klug CS, Shapiro L, Mancia F. Crystal Structure of the Bacterial Aminoarabinose Transferase Arnt.
Raguz M, Ilic N, Kumar S, Zereba M, Mainali L, Subczynski WK. Cholesterol Bilayer Domain in Phospholipid Bilayer Membranes can be Detected by Confocal Microscope.
249th American Chemical Society National Meeting & Exposition, Denver, CO | March 21-24, 2015
Lane AC, Antholine WE, Walensky JR. Reactivity of Group 11 Formamidinate Complexes
57th Rocky Mountain Conference on Magnetic Resonance / 39th International EPR Symposium, Breckenridge, CO | July 26-31, 2017
Lane AC, Barnes CL, Antholine WE, Fiedler AT, Walensky JR. EPR of Di- and TriNuclear Mixed-Valence Copper Amidinate Complexes from Reduction of Iodine
EPR Methodologies Enabled by Loop-Gap Resonators, Milwaukee, WI | August 22-23, 2015
Camenisch TG, Antholine WE, Ratke JJ, Hyde JS, 500-1000 MHz Parallel Ho EPR Spectrometer Station for Forbidden Transitions
Camenisch TG, Ratke JJ, Hyde JS, X-band Saturation-Recovery System
Camenisch TG, Sidabras JW, Ratke JJ, Thelaner T, Fessler R, Hyde JS, X-band Bridge and Spectrometer Station for NARS and CW EPR
Kowalzki JM, Kittell A, Sidabras JW, Bennett B, Hyde JS, Low Frequency CU(II) and NARS
Mainali L, Hyde JS, Subczynski WK, Membrane Fluidity Profiles as Deduced by Saturation-Recovery EPR Measurements of Spin-Lattice Relaxation Times of Spin Labels: Multifrequency Approach
Mainali L, Sidabras JW, Camenisch TG, Vasquez-Vivar J, Hyde JS, Subczynski WK, Spin-labeled Uni-lamellar Vesicles as an Oxygen Sensitive Analyte for Measurement of Cellular Respiration using Rat Dopaminergic Neuronal Cells
Mainali L, Sidabras J, Camenisch TG, Hyde JS, Subczynski WK, W-band EPR: Applications to Model and Biological Samples of Small Volumes
Mett RR, Sidabras JW, Anderson JR, Strangeway RR, Mainali L, Hyde JS, Hyperbolic-Cosine Waveguide Tapers and Oversize Rectangular Waveguide for Reduced Broadband Insertion Loss in W-band EPR II: Implementation
Mett RR, Sidabras JW, Hyde JS. Simulations of Electromagnetic Fields in the Dielectric Loop-Gap Resonator (dLGR)
Sidabras JW, Non-Adiabatic Rapid Sweep (NARS)
Sidabras JW, Mett RR, Hyde JS. Resonator Development from 1 to 140 GHz
Strangeway RA, Camenisch TG, Hyde JS, D-band Microwave Bridge Demonstration
Zielonka J, Sikora A, Podsiadly R, Joseph J, Kalyanaraman B, Phenyl Radicals as Reactive Intermediates in the Reaction of Boronates with Peroxynitrite - EPR Spin Trapping Study
First Adriatic Symposium on Biophysical Approached in Biomedical Studies, Split, Croatia | August 24-29, 2014
Feix JB. Membrane Structure and Dynamics and the Development of New Chemotherapeutic Agent.
Feix JB. Selectivity and Membrane Interaction of D-amino Acid Substituted Antimicrobial Peptides.
Hyde JS. New Non-adiabatic Rapid Sweep (NARS) Spectroscopic EPR Methods.
Kalyanaraman B. Profiling and Targeting of Cellular Bioenergetics. Inhibition of Pancreatic Cancer Cell Proliferation.
Subczynski WK. High Membrane Cholesterol Content and Oxidative Stress in Health and Disease.
Vasquez-Vivar J. Tetrahydro-biopterin Inhibits Superoxide Release from Glutathionylated-eNOS.
Zielonka J. Free Radical Metabolism and Bioenergetic Changes in Cardiovascular Diseases and Cancer.
IX International Workshop on EPR in Biology and Medicine. Krakow, Poland, October 7-10, 2013
Hyde JS. Segmental Acquisition of Pure Absorption and Pure Dispersion EPR Spectra using Time Averaging.
Kalyanaraman B. Targeting Bioenergetics, Metabolism, and Redox Mechanism to Selectively Inhibit Pancreatic Cancer Cell Proliferation.
Kittell A. Resolution Enhancement by Differential Power Saturation Using Non-adiabatic Rapid Sweep (NARS) Electron Paramagnetic Resonance (EPR) Spectroscopy.
Mainali L. W-band EPR: Application to High Cholesterol Containing Membranes.
Sidabras JW. Novel Resonator Design at W-band (94 GHz) for Electron Paramagnetic Resonance Spectroscopy.
Subczynski WK. High Cholesterol in Model and Biological Membranes: Effects on Health and Disease.
Vasquez-Vivar J. Mitochondrial Targeted Nitrones: Use and Limitations in the Study of Mitochondrial Derived Free Radicals.
Zielonka J. High Throughput Screening of Inhibitors of NADPH Oxidases (Nox) -- A Combination of Fluorescence, HPLC and EPR Assays for Reliable Identification of Novel Nox Inhibitors.
Talks
Montana State University, Bozeman, MT | March 6, 2012
Bennett B. Biological EPR: Old Tricks and New Horizons.
University of Montana, Missoula, MT | March 8, 2012
Bennett B. Biological EPR: Old Tricks and New Horizons.
Medical College of Wisconsin Cancer Cell Biology Research Forum, Milwaukee, WI | March 22, 2012
Antholine WE. How EPR, Electron Paramagnetic Resonance, Contributed to the Study of Anti-tumor Agents Such as Gallium, Bleomycin, Triapine.
4th International Trends in Enzymology Conference: Going Beyond Frontiers, Göttingen, Germany | June 4, 2012
Bennett B. Using EPR to Probe Zinc Enzymes: Old Tricks and New Horizons.
54th Rocky Mountain Conference on Analytical Chemistry, Copper Mountain, CO | July 15-19, 2012
Mainali L, Hyde JS, Subczynski WK. Spin-label W-band EPR as a Powerful Tool for Studying Membrane Fluidity Profiles in Samples of Small Volume.” (Invited Award Talk)
Leading the Way: A Joint Conference of the Advancing a Healthier Wisconsin and the Wisconsin Partnership Programs, Milwaukee, WI | September 27, 2012
Bennett B. Developing Electron Paramagnetic Resonance Tools for Biomarkers of Neurodegeneration.
Posters
Annual Meeting of the Biophysical Society, San Diego, CA | February 25-29, 2012
Mainali L, Raguz M, Subczynski WK. Phase boundaries in phosphatidylcholine membranes saturated and oversaturated with Cholesterol.
Mainali L, Hyde JS, Subczynski WK. Membrane Fluidity Profiles as Deduced by Saturation-Recovery EPR Measurements of Spin-Lattice Relaxation Times of Spin Labels: Multifrequency Approach.
Dellisanti CD, Raspanti JM, Grant VA, Hanson SM, Ghosh B, Satyshur KE, Klug CS, Czajkowski C. Site-Directed Spin Labeling Reveals Pentameric Ligand-Gated Ion Channel Gating Motions.
54th Rocky Mountain Conference on Analytical Chemistry, Copper Mountain, CO | July 15-19, 2012
Mainali L, Raguz M, Subczynski WK. Application of EPR Spin-Labeling Methods to Intact Lens Membranes.
Mett RR, Anderson JR, Sidabras JW, Hyde JS. Resonator for Optimization of Liquid-Phase EPR Concentration-Sensitivity for Spin Labels at Q-band: Preliminary Results. (PPTX)
Annual P41 Directors Meeting, Bethesda, MD | 2012
Hyde JS. Non-adiabatic Rapid Scanning EPR.
Talks
Department of Chemistry, Marquette University, Milwaukee, WI | April 8, 2011
Bennett B. Electron Paramagnetic Resonance of Metal Ions: Towards in Vivo Structural Biology.
Department of Biological Sciences, University of Wisconsin-Parkside, Kenosha, WI | April 22, 2011
Bennett B. Electron Paramagnetic (Spin) Resonance in Biological Environments.
Association for Research in Vision and Ophthalmology Annual Meeting 2011, Fort Lauderdale, FL | May 1-5, 2011
Raguz M, Krpan I, O’Brien WJ, Mainali L, Subczynski WK. Physical Properties of the Cortical and Nuclear Fiber-Cell Plasma Membranes from the Human Eye Lens.
40th Southeastern Magnetic Resonance Conference, Atlanta, GA | November 4, 2011
Bennett B. EPR Approaches for Biomedicine at the National Biomedical EPR Center.
Posters
Annual Meeting of the Biophysical Society, Baltimore, MD | March 5-9, 2011
Alvarez FJ, Orelle C, Klug CS, Davidson AL. Both Lobes of Maltose Binding Protein are Engaged in Stabilization of the Semi-open State of the MalK Dimer in the Maltose ABC Transporter.
Mainali L, Raguz M, Subczynski WK. Organization and Dynamics of Cholesterol Crystalline Domains Using EPR Spin-Labeling.
Mainali L, Raguz M, Subczynski WK. Discrimination and Characterization of Cholesterol Crystalline Domains Using EPR Spin-Labeling: Application to Lens Lipid Membranes.
Experimental Biology, Washington, DC | April 9-11, 2011
Zhuo Y, Zhan X, Hanson SM, Warthaka M, Dalby KN, Gurevich VV, Klug CS. Identification of ERK2-Arrestin-3 Interaction Sites.
Nicotinic Acetylcholine Receptors 2011, Cambridge, UK | May 18-21, 2011
Dellisanti CD, Raspanti JM, Grant VA, Hanson SM, Ghosh B, Klug CS, Czajkowski C. Site-Directed Spin Labeling Electron Paramagnetic Resonance Spectroscopy Reveals Proton-Induced Movement of Loop 9 in GLIC.
53rd Rocky Mountain Conference on Analytical Chemistry, Snowmass, CO | July 2011
Konovalov A, Raguz M, Krpan I, Subczynski WK. Physical Properties of Membrane Models of the Eye-Lens Fiber-Cell Plasma membrane for Animals of Different Life Spans: Saturation-Recovery EPR Spin-Labeling Studies.
Mett RR, Sidabras JW, Hyde JS. Hyperbolic Cosine Waveguide Tapers and Oversize Rectangular Waveguide for Reduced Broadband Insertion Loss in W-band EPR Spectroscopy. (PPTX)
Mett RR, Anderson JR, Sidabras JW, Thelaner TS, Hyde JS. Resonator for Optimization of Liquid-Phase EPR Concentration-Sensitivity for Spin Labels at Q-band: Practical Considerations. (PPTX)
Sidabras JW, Mett RR, Anderson JR, Hyde JS. Resonator Advances for W-band Pulse Spectroscopy.
Sidabras JW, Mett RR, Varanasi SK, Swartz HM, Hyde JS. Microwave Surface Resonator Array for In Vivo Electron Paramagnetic Resonance Spectroscopy.
Neuroscience 2011, Washington, DC | November 12-16, 2011
Dellisanti CD, Raspanti JM, Grant VA, Hanson SM, Ghosh B, Klug CS, Czajkowski C. Site-Directed Spin Labeling Electron Paramagnetic Resonance Spectroscopy Reveals Proton-Induced Movement of Loop 9 in GLIC.
Talks
APS March Meeting, Portland, OR | March 15-19, 2010
Mainali L, Sahu ID, Earle KA. EPR Data Analysis on Copper Complexes using Bayesian Inference, Nonlinear-Least Squares and Statistical Geometry.
Sahu IS, Mainali L, Earle KA. EPR Spectral Analysis of Vanadyl Model Compounds via Bayesian Inference, Nonlinear-Least Squares and Statistical Geometry.
Marquette University College of Engineering | April 9, 2010
Mett RR. Exponential Waveguide Taper and Oversize Waveguide for Reduced Losses and Improved Signal-to-Noise Ratio in EPR Spectroscopy. (PPTX)
Joint Conference of the 14th In Vivo EPR Spectroscopy and Imaging and the 11th International EPR Spin Trapping/Spin Labeling, San Juan, Puerto Rico | May 2-6, 2010
Antholine W. Multifrequency EPR of Cupric Sites: Four Coordinate, Three Coordinate, and Free Radical Like Sites. (PDF)
Bennett B. Rapid-Freeze-Quench EPR: What, Why, and How. (PDF)
Feix JB. Structure and Conformational Dynamics of the Pseudomonas aeruginosa Toxin , ExoU. (PDF)
Hyde JS. Experiments at Low Microwave Frequencies. (PDF)
Joseph J. Lopez M, Chen G, Prah D, Ramadoss J, Zielonka J, Kalyanaraman B. Mitochondria-Targeted Nitroxides: Synthesis and Biological Applications. (PDF)
Sidabras JS, Mett RR, Hyde JS. Rapid Development of EPR Resonators Using Finite-Element Modeling. (PDF)
Subczynski WK, Ragua M, Mainali L. Physical Properties of Lipid Bilayers Using Spin Label EPR. (PDF)
Vasquez-Vivar J. Whitsett J, Yu L, Ji X, Tan S. Deficient Tetrahydrobiopterin Explains the Double-Hit of Fetal Brain Damage in Hypoxia-Ischemia: A Role for NOS Uncoupling. (PDF)
Zielonka J. Detection of Peroxynitrite by Boronate-Based Probes: EPR, Fluorescence and HPLC Studies. (PDF)
VIII International Workshop on EPR in Biology and Medicine, Krakow, Poland | October 5, 2010
Hyde JS. Pure Absorption Rapid Scan (PARS) EPR Spectroscopy.
Klug CS. Effects of Dysfunctional Mutations on the Function of the Bacterial ABC Transporter MsbA. Distance Measurements.
Subczynski WK. Spin-Label Oximetry at X-band, Q-band, and W-band.
Posters
Annual Meeting of the Biophysical Society, San Francisco, CA | February 20-24, 2010
Gadicherla AK, Antholine WE, Camara AKS, Heisner JS, Aldakkak M, Stowe DF. Free Radical Generation and Electron Flux in Mitochondrial Fe-S Centers During Cardiac Injury: Changes with Mitochondrial Protective Drug Ranolazine.
Kaminski H, Feix JB. Biophysical Studies of Cecropin-Mellitin Antimicrobial Peptides with Improved Selectivity.
Westfahl KM, Merten JA, Klug CS. Characterization of the L511P and D512G Mutations in the MsbA Lipid Flippase.
Joint Conference of the 14th In Vivo EPR Spectroscopy and Imaging and the 11th International EPR Spin Trapping/Spin Labeling, San Juan, Puerto Rico | May 2-6, 2010
Kittell AW, Sidabras JW, Hyde JS. Measuring Dipolar Distances Using Low Frequency (1.8 GHz) Continuous Wave EPR. (PDF)
Westfahl KM, Merten J, Klug CS. Characterization of the H537A Mutation in the ABC Transporter MSBA. (PDF)
Zielonda J, Sikora A, Lopez M, Joseph J, Kalyanaraman B. Detection of Free-Radical Intermediates and Products derived from the Reaction Between Boronates and Peroxynitrite-EPR Spin-Trapping and HPLC Studies.
52nd Rocky Mountain Conference on Analytical Chemistry, Snowmass, CO | July 2010
Mainali L, Raguz M, Subczynski WK. New Approaches in Discrimination and Characterization of Membrane Domains Using EPR Spin Labeling Methods.
Mainali L, Raguz M, Subczynski WK. Membrane Domains in Sphingomyelin/Cholesterol Membranes: Their Structure and Properties Using EPR Spin Labeling Methods.
Mett RR, Anderson JR, Sidabras JW, Hyde JS. Resonator for Optimization of Liquid-Phase EPR Concentration-Sensitivity for Spin Labels at Q-band. (PPTX)
Sidabras JW, Mett RR, Hyde JS. Advances in Loop-Gap Resonator Technology for X-band Aqueous Samples.
Annual P-41 Directors Meeting Bethesda, MD | 2010
Hyde JS, Kittell AW, Camenisch TG. Nitroxide Line-Shape Analysis with X-band Pure Absorption Rapid Scan (PARS) Electron Paramagnetic Resonance (EPR).
MCW Research Poster Day, Milwaukee, WI | September 15, 2010
Zhuo Y, Zhan Z, Gurevich VV, Klug CS. Identification of Interaction Sites in Arrestin3 upon Binding to ERK2 and JNK3.
Schultz KM, Merten JA, Klug CS. Characterization of Dysfunctional Mutations in the ABC Transporter MsbA.
VIII International Workshop on EPR in Biology and Medicine, Krakow, Poland | October 2010
Widomska J, Raguz M, Subczynski WK. Oxygen Transport in Lipid Bilayer Membranes.
Raguz M, Mainali L, Widomska J, Subczynski WK. Spin-Label Oximetry in Discrimination and Characterization of Cholesterol Crystalline Domain.
Wisniewska A, Widomska J, Subczynski WK. The Liquid-Ordered-Like Phase in Xanthophyll Containing Membranes as Examined by Pulse-EPR Oxygen Probing.
Talks
Milwaukee School of Engineering | September 2, 2009
Mett RR, Gassert JD, Strangeway RA. EPR at MCW (faculty inservice presentation).
Acert Workshop Multi-frequency ESR/EPR, Cornell University, Ithaca, NY | November 15-17, 2009
Subczynski WK. Three-Dimensional Dynamic Structure of Lipid Bilayer Membranes Saturated with Cholesterol. Promising Advances at Q-band and W-band.
Posters
Biophysical Society Annual Meeting
Impellitteri NA, Merten JA, Bretscher LE, Klug CS. Identification of Functionally Important Sites within the Cysteine-Free Inner Membrane Transferase Protein ArnT.
Raguz M, Widomska J, Subczynski WK. Three-Dimensional Dynamic Structure of Phospholipid Bilayer Saturated with Cholesterol.
51st Rocky Mountain Conference on Analytical Chemistry, Snowmass, CO | July 21, 2009
Mett RR, Sidabras JW, Hyde JS. X-Band 2-Loop-1-Gap LGR and Long-Slot Iris for Reduced Frequency Pulling.
Mett RR, Sidabras JW, Hyde JS. Use of Oversize Rectangular WR-28 Waveguide at W-band for Low-Loss and Increased Signal-to-Noise Ratio.
Sidabras JW, Mett RR, Lesniewski PN, Swartz HM, Hyde JS. Counter-Rotating Current Microwave Resonator for In Vivo EPR Spectroscopy.
Annual P-41 Directors Meeting, Bethesda, MD
Bennett B, Kowalski JM, Antholine WE, Sidabras JW, Anderson J, Walter ED, Millhauser GL, Hyde JS. Development of EPR of Cu(II) at 1 - 3 GHz for the Study of Proteins Involved in Neurodegenerative Diseases.
Hyde JS, Froncisz W, Camensich TG, Strangeway RA. EPR Correlation Spectroscopy Using Rapid Radio Frequency Sweep at W-band.