
Alison Kriegel, PhD
Associate Professor
Locations
- Physiology
Contact Information
Education
Research Experience
- Cardiovascular Physiological Phenomena
- Cell Communication
- Cell Physiological Phenomena
- Hypertension
- MicroRNA
- Molecular Biology
- Paracrine Communication
- Physiological Genomics
- Renal Physiology
Methodologies and Techniques
- miRNA analysis
- Models of Cardiac Pathology
- Models of Renal Pathology
- Next-generation RNA Sequencing
- qRT-PCR
- RNA analysis
- Rodent Echocardiography
- Rodent Left Ventricle Pressure-Volume Loop Analysis
- Western Blot
Research Interests
Cardiovascular Physiology | Genetics and Genomics | Molecular and Cellular Physiology | Renal Physiology
Our research is broadly centered on understanding how alterations in microRNAs and protein coding genes influence cardiovascular disease, renal disease, and cardiorenal syndromes. Cardiorenal syndromes are a grouping of human clinical conditions where primary disease in either the heart or the kidney contributes to the development of secondary disease in the other organ.
Our current focus is on cardiorenal syndrome type 4 (CRS4), also known as chronic renocardiac syndrome, a condition in which chronic kidney disease (CKD) contributes to the development of cardiovascular diseases including hypertrophy, diastolic dysfunction, reduction in cardiac function and increased risk of cardiovascular events.
CRS4 is clinical problem that has received a great deal of attention in recent years because of the large number of people impacted and the high associated healthcare costs. In 2010 the Centers for Disease Control estimated that more than 10% of adults in the United States population chronic kidney disease, and cardiovascular pathology is leading cause of death in these patients. Treatment of CRS4 remains challenging because so little is understood about the pathology of the disease. Adult patients with CKD often have age associated comorbidities, such as diabetes, hypertension, and atherosclerosis, making it difficult to identifying mediators of the pathology in this population.
Our goals are focused on identifying the essential components of cardiorenal syndromes and their therapeutic targets. We have started to investigate the molecular mediators of this conditions using a 5/6 nephrectomy (5/6 NX) model of CKD in Sprague Dawley rats. This model of CKD allows us to study CRS4 related pathologies in the absence of confounding comorbidities that would independently impact the heart, such as atherosclerosis, diabetes and hypertension.
Our current goals in CRS4 research interest include:
We combine in vivo approaches for studying cardiac and renal function with advanced molecular techniques to comprehensively study the factors that influence left ventricular pathology. Frequently utilized techniques in our laboratory include: echocardiography, left ventricle pressure-volume relationship analysis, chronic and acute blood pressure recordings, cell culture models (siRNA, miRNA), in vitro and in vivo miRNA suppression, immunohistochemistry, in situ hybridization, western blot analysis, ELISA, miRNA and mRNA next generation sequencing and qRT-PCR.
Research for this project is supported an American Heart Association Scientist Development Grant (13SDG17100095).
Supporting documents:
Supplemental Table 1 (XLSX)
Supplemental Table 3 (XLSX)
Supplemental Table 4 (XLSX)
Students with an interest in this research are encouraged to contact Dr. Kriegel at akriegel@mcw.edu.
Publications
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System-wide transcriptome damage and tissue identity loss in COVID-19 patients
(Park J, Foox J, Hether T, Danko DC, Warren S, Kim Y, Reeves J, Butler DJ, Mozsary C, Rosiene J, Shaiber A, Afshin EE, MacKay M, Rendeiro AF, Bram Y, Chandar V, Geiger H, Craney A, Velu P, Melnick AM, Hajirasouliha I, Beheshti A, Taylor D, Saravia-Butler A, Singh U, Wurtele ES, Schisler J, Fennessey S, Corvelo A, Zody MC, Germer S, Salvatore S, Levy S, Wu S, Tatonetti NP, Shapira S, Salvatore M, Westblade LF, Cushing M, Rennert H, Kriegel AJ, Elemento O, Imielinski M, Rice CM, Borczuk AC, Meydan C, Schwartz RE, Mason CE.) Cell Reports Medicine. 15 February 2022;3(2) SCOPUS ID: 2-s2.0-85124273639 02/15/2022
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Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications.
(Moore KJM, Cahill J, Aidelberg G, Aronoff R, Bektaş A, Bezdan D, Butler DJ, Chittur SV, Codyre M, Federici F, Tanner NA, Tighe SW, True R, Ware SB, Wyllie AL, Afshin EE, Bendesky A, Chang CB, Dela Rosa R 2nd, Elhaik E, Erickson D, Goldsborough AS, Grills G, Hadasch K, Hayden A, Her SY, Karl JA, Kim CH, Kriegel AJ, Kunstman T, Landau Z, Land K, Langhorst BW, Lindner AB, Mayer BE, McLaughlin LA, McLaughlin MT, Molloy J, Mozsary C, Nadler JL, D'Silva M, Ng D, O'Connor DH, Ongerth JE, Osuolale O, Pinharanda A, Plenker D, Ranjan R, Rosbash M, Rotem A, Segarra J, Schürer S, Sherrill-Mix S, Solo-Gabriele H, To S, Vogt MC, Yu AD, Mason CE, gLAMP Consortium.) J Biomol Tech. 2021 09;32(3):228-275 PMID: 35136384 PMCID: PMC8802757 SCOPUS ID: 2-s2.0-85124281562 02/10/2022
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Sexual dimorphism in the progression of type 2 diabetic kidney disease in T2DN rats.
(Spires DR, Palygin O, Levchenko V, Isaeva E, Klemens CA, Khedr S, Nikolaienko O, Kriegel A, Cheng X, Yeo JY, Joe B, Staruschenko A.) Physiol Genomics. 2021 06 01;53(6):223-234 PMID: 33870721 PMCID: PMC8285576 SCOPUS ID: 2-s2.0-85108022875 04/20/2021
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(Park J, Foox J, Hether T, Danko D, Warren S, Kim Y, Reeves J, Butler DJ, Mozsary C, Rosiene J, Shaiber A, Afshinnekoo E, MacKay M, Bram Y, Chandar V, Geiger H, Craney A, Velu P, Melnick AM, Hajirasouliha I, Beheshti A, Taylor D, Saravia-Butler A, Singh U, Wurtele ES, Schisler J, Fennessey S, Corvelo A, Zody MC, Germer S, Salvatore S, Levy S, Wu S, Tatonetti N, Shapira S, Salvatore M, Loda M, Westblade LF, Cushing M, Rennert H, Kriegel AJ, Elemento O, Imielinski M, Borczuk AC, Meydan C, Schwartz RE, Mason CE.) bioRxiv. 2021 Mar 09 PMID: 33758858 PMCID: PMC7987017 03/25/2021
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(Klemens CA, Chulkov EG, Wu J, Hye Khan MA, Levchenko V, Flister MJ, Imig JD, Kriegel AJ, Palygin O, Staruschenko A.) Hypertension. 2021 02;77(2):582-593 PMID: 33390052 PMCID: PMC7856014 SCOPUS ID: 2-s2.0-85100070512 01/05/2021
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(Adam RJ, Paterson MR, Wardecke L, Hoffmann BR, Kriegel AJ.) Kidney360. 2020 Oct;1(10):1105-1115 PMID: 34263177 PMCID: PMC8276873 07/16/2021
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The COVID-19 XPRIZE and the need for scalable, fast, and widespread testing.
(MacKay MJ, Hooker AC, Afshinnekoo E, Salit M, Kelly J, Feldstein JV, Haft N, Schenkel D, Nambi S, Cai Y, Zhang F, Church G, Dai J, Wang CL, Levy S, Huber J, Ji HP, Kriegel A, Wyllie AL, Mason CE.) Nat Biotechnol. 2020 09;38(9):1021-1024 PMID: 32820257 PMCID: PMC7543743 SCOPUS ID: 2-s2.0-85089701507 08/21/2020
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(Paterson MR, Geurts AM, Kriegel AJ.) Kidney Int. 2019 12;96(6):1332-1345 PMID: 31668631 PMCID: PMC6941490 SCOPUS ID: 2-s2.0-85074541202 11/02/2019
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(Abais-Battad JM, Alsheikh AJ, Pan X, Fehrenbach DJ, Dasinger JH, Lund H, Roberts ML, Kriegel AJ, Cowley AW Jr, Kidambi S, Kotchen TA, Liu P, Liang M, Mattson DL.) Hypertension. 2019 10;74(4):854-863 PMID: 31476910 PMCID: PMC6739138 SCOPUS ID: 2-s2.0-85072134357 09/04/2019
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Detrimental effects of chemotherapy on human coronary microvascular function.
(Hader SN, Zinkevich N, Norwood Toro LE, Kriegel AJ, Kong A, Freed JK, Gutterman DD, Beyer AM.) Am J Physiol Heart Circ Physiol. 2019 10 01;317(4):H705-H710 PMID: 31397169 PMCID: PMC6843017 SCOPUS ID: 2-s2.0-85072508643 08/10/2019
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Adverse early-life environment impairs postnatal lung development in mice.
(Lai PY, Jing X, Michalkiewicz T, Entringer B, Ke X, Majnik A, Kriegel AJ, Liu P, Lane RH, Konduri GG.) Physiol Genomics. 2019 09 01;51(9):462-470 PMID: 31373541 PMCID: PMC6766700 SCOPUS ID: 2-s2.0-85072265843 08/03/2019
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(Liu Y, Kriegel AJ, Liang M.) Methods Mol Biol. 2019;2018:177-194 PMID: 31228157 SCOPUS ID: 2-s2.0-85068180336 06/23/2019