Alison Kriegel, PhD

Associate Professor

Locations

Physiology

Contact Information

Education

PhD, Physiology, Medical College of Wisconsin, 2008

Research Interests

Our research is broadly centered on understanding how alterations in microRNAs and protein coding genes influence pathology and organ function in cardiovascular disease, renal disease, and cardiorenal syndromes. Our laboratory takes an integrative physiology approach to research, recognizing the interconnectedness of molecular pathways and organ systems in physiology and pathophysiology.

Major areas of research:

Chronic Kidney Disease (CKD) and Metabolic Changes in CKD
Cardiorenal Syndrome, Hypertension, and Cardiovascular Disease
Polycystic Kidney Disease
Sex Differences in (Patho)Physiology
Cardio-Oncology

We combine in vivo approaches for studying cardiac and renal function with advanced molecular techniques to comprehensively study the factors that influence left ventricular pathology. Frequently utilized techniques in our laboratory include: echocardiography, left ventricle pressure-volume relationship analysis, chronic and acute blood pressure recordings, sodium handling/GFR assessment, cell culture models (siRNA, miRNA), in vitro and in vivo miRNA/mRNA suppression, immunohistochemistry, in situ hybridization, immuno/affinity purification, western blot analysis, ELISA, proteomic/metabolomic analysis, miRNA and mRNA next generation sequencing and qRT-PCR.

Please see the Kriegel lab website for additional information about our group and research.

Students and researchers interested in learning more about our dynamic research program are encouraged to contact Dr. Kriegel at akriegel@mcw.edu.

Fetal pulmonary hypertension: dysregulated microRNA-34c-Notch1 axis contributes to impaired angiogenesis in an ovine model.

(Mukherjee D, Rana U, Kriegel AJ, Liu P, Michalkiewicz T, Konduri GG.) Pediatr Res. 2023 Feb;93(3):551-558 PMID: 35717485 PMCID: PMC9759620 SCOPUS ID: 2-s2.0-85132167802 06/19/2022
Broad-acting therapeutic effects of miR-29b-chitosan on hypertension and diabetic complications.

(Jensen DM, Han P, Mangala LS, Lopez-Berestein G, Sood AK, Liu J, Kriegel AJ, Usa K, Widlansky ME, Liang M.) Mol Ther. 2022 Nov 02;30(11):3462-3476 PMID: 35965413 PMCID: PMC9637778 SCOPUS ID: 2-s2.0-85136662053 08/16/2022
Comparison of the surgical resection and infarct 5/6 nephrectomy rat models of chronic kidney disease.

(Adam RJ, Williams AC, Kriegel AJ.) Am J Physiol Renal Physiol. 2022 Jun 01;322(6):F639-F654 PMID: 35379002 PMCID: PMC9076416 SCOPUS ID: 2-s2.0-85129997035 04/06/2022
Cardiometabolic effects of DOCA-salt in male C57BL/6J mice are variably dependent on sodium and nonsodium components of diet.

(Patil CN, Ritter ML, Wackman KK, Oliveira V, Balapattabi K, Grobe CC, Brozoski DT, Reho JJ, Nakagawa P, Mouradian GC Jr, Kriegel AJ, Kwitek AE, Hodges MR, Segar JL, Sigmund CD, Grobe JL.) Am J Physiol Regul Integr Comp Physiol. 2022 Jun 01;322(6):R467-R485 PMID: 35348007 PMCID: PMC9054347 SCOPUS ID: 2-s2.0-85129779185 03/30/2022
Effects of elevation of ANP and its deficiency on cardiorenal function.

(Ilatovskaya DV, Levchenko V, Winsor K, Blass GR, Spires DR, Sarsenova E, Polina I, Zietara A, Paterson M, Kriegel AJ, Staruschenko A.) JCI Insight. 2022 May 09;7(9) PMID: 35380994 PMCID: PMC9090260 SCOPUS ID: 2-s2.0-85129984120 04/06/2022
Acclimation to a High-Salt Diet Is Sex Dependent.

(Gohar EY, De Miguel C, Obi IE, Daugherty EM, Hyndman KA, Becker BK, Jin C, Sedaka R, Johnston JG, Liu P, Speed JS, Mitchell T, Kriegel AJ, Pollock JS, Pollock DM.) J Am Heart Assoc. 2022 Mar;11(5):e020450 PMID: 35191321 PMCID: PMC9075092 SCOPUS ID: 2-s2.0-85125554109 02/23/2022
System-wide transcriptome damage and tissue identity loss in COVID-19 patients.

(Park J, Foox J, Hether T, Danko DC, Warren S, Kim Y, Reeves J, Butler DJ, Mozsary C, Rosiene J, Shaiber A, Afshin EE, MacKay M, Rendeiro AF, Bram Y, Chandar V, Geiger H, Craney A, Velu P, Melnick AM, Hajirasouliha I, Beheshti A, Taylor D, Saravia-Butler A, Singh U, Wurtele ES, Schisler J, Fennessey S, Corvelo A, Zody MC, Germer S, Salvatore S, Levy S, Wu S, Tatonetti NP, Shapira S, Salvatore M, Westblade LF, Cushing M, Rennert H, Kriegel AJ, Elemento O, Imielinski M, Rice CM, Borczuk AC, Meydan C, Schwartz RE, Mason CE.) Cell Rep Med. 2022 Feb 15;3(2):100522 PMID: 35233546 PMCID: PMC8784611 SCOPUS ID: 2-s2.0-85124273639 03/03/2022
System-wide transcriptome damage and tissue identity loss in COVID-19 patients

(Park J, Foox J, Hether T, Danko DC, Warren S, Kim Y, Reeves J, Butler DJ, Mozsary C, Rosiene J, Shaiber A, Afshin EE, MacKay M, Rendeiro AF, Bram Y, Chandar V, Geiger H, Craney A, Velu P, Melnick AM, Hajirasouliha I, Beheshti A, Taylor D, Saravia-Butler A, Singh U, Wurtele ES, Schisler J, Fennessey S, Corvelo A, Zody MC, Germer S, Salvatore S, Levy S, Wu S, Tatonetti NP, Shapira S, Salvatore M, Westblade LF, Cushing M, Rennert H, Kriegel AJ, Elemento O, Imielinski M, Rice CM, Borczuk AC, Meydan C, Schwartz RE, Mason CE.) Cell Reports Medicine. 15 February 2022;3(2) SCOPUS ID: 2-s2.0-85124273639 02/15/2022
Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications.

(Moore KJM, Cahill J, Aidelberg G, Aronoff R, Bektaş A, Bezdan D, Butler DJ, Chittur SV, Codyre M, Federici F, Tanner NA, Tighe SW, True R, Ware SB, Wyllie AL, Afshin EE, Bendesky A, Chang CB, Dela Rosa R 2nd, Elhaik E, Erickson D, Goldsborough AS, Grills G, Hadasch K, Hayden A, Her SY, Karl JA, Kim CH, Kriegel AJ, Kunstman T, Landau Z, Land K, Langhorst BW, Lindner AB, Mayer BE, McLaughlin LA, McLaughlin MT, Molloy J, Mozsary C, Nadler JL, D'Silva M, Ng D, O'Connor DH, Ongerth JE, Osuolale O, Pinharanda A, Plenker D, Ranjan R, Rosbash M, Rotem A, Segarra J, Schürer S, Sherrill-Mix S, Solo-Gabriele H, To S, Vogt MC, Yu AD, Mason CE, gLAMP Consortium.) J Biomol Tech. 2021 Sep;32(3):228-275 PMID: 35136384 PMCID: PMC8802757 SCOPUS ID: 2-s2.0-85124281562 02/10/2022
Sexual dimorphism in the progression of type 2 diabetic kidney disease in T2DN rats.

(Spires DR, Palygin O, Levchenko V, Isaeva E, Klemens CA, Khedr S, Nikolaienko O, Kriegel A, Cheng X, Yeo JY, Joe B, Staruschenko A.) Physiol Genomics. 2021 Jun 01;53(6):223-234 PMID: 33870721 PMCID: PMC8285576 SCOPUS ID: 2-s2.0-85108022875 04/20/2021
Systemic Tissue and Cellular Disruption from SARS-CoV-2 Infection revealed in COVID-19 Autopsies and Spatial Omics Tissue Maps.

(Park J, Foox J, Hether T, Danko D, Warren S, Kim Y, Reeves J, Butler DJ, Mozsary C, Rosiene J, Shaiber A, Afshinnekoo E, MacKay M, Bram Y, Chandar V, Geiger H, Craney A, Velu P, Melnick AM, Hajirasouliha I, Beheshti A, Taylor D, Saravia-Butler A, Singh U, Wurtele ES, Schisler J, Fennessey S, Corvelo A, Zody MC, Germer S, Salvatore S, Levy S, Wu S, Tatonetti N, Shapira S, Salvatore M, Loda M, Westblade LF, Cushing M, Rennert H, Kriegel AJ, Elemento O, Imielinski M, Borczuk AC, Meydan C, Schwartz RE, Mason CE.) bioRxiv. 2021 Mar 09 PMID: 33758858 PMCID: PMC7987017 03/25/2021
Functionally Essential Tubular Proteins Are Lost to Urine-Excreted, Large Extracellular Vesicles during Chronic Renal Insufficiency.

(Adam RJ, Paterson MR, Wardecke L, Hoffmann BR, Kriegel AJ.) Kidney360. 2020 Oct;1(10):1105-1115 PMID: 34263177 PMCID: PMC8276873 07/16/2021