Agilent Cell Analysis Webinar Series
What is Beyond Traditional Cell Culture with Seahorse XF Technology?
Jay Dunn, PhD, Applications Scientist Cell Analysis, Agilent; Bahar Rizi, PhD, Agilent product Specialist— Seahorse Applications, Instruments, and Consumables; Erica Underwood, PhD, Faculty Instructor and Researcher at the University of Texas Health Sciences’ McGovern Medical School in Houston
Agilent Seahorse XF Technology has been at the forefront of metabolic research for nearly 15 years. With its ability to measure glycolytic and mitochondrial function simultaneously, great insights into the metabolic drivers of cell fate, function, and pathology have been made.
Most researchers use Seahorse XF Instruments to analyze metabolism in primary or cultured cells, either adherent or suspension. However, some questions are best addressed in systems that can be described as 3-dimensional and can include spheroids, tissue slices, and whole organoid or organs such as islets or fly brains. The researcher may want to better understand whole organ metabolism in order to better model disease pathology, or to characterize candidate therapeutics.
In this webinar, Drs. Bahar Rizi and Jay Dunn, from Agilent Cell Analysis, will discuss different methods for running assays with 3-D samples. Additionally, they will present several examples from the literature where researchers have assayed spheroids, organisms such as zebrafish embryos and C. elegans nematodes, and tissues using different specialty plates made by Agilent.
Dr. Erica Underwood, a faculty instructor and researcher at the University of Texas Health Sciences’ McGovern Medical School in Houston will also present her work, recently published in Nature Scientific Reports, in traumatic brain injury models. She has adapted methods that allow to her to assay specific regions of the brain from up to twelve animals simultaneously. She will discuss not only how to run these kinds of samples, but the impact on mitochondrial function that she observed after induced brain trauma.