Students Classroom

Agilent Cell Analysis Virtual Seminar

February 2, 2021 | 11:00 am–12:00 pm MCW_Icons_Make_An_Appointment_Green iCAL

Recent Advances in Assessing Metabolic Status of iPSC-derived Cardiomyocytes

In 2006, Dr. Shinya Yamanaka and Dr. Kazutoshi Takahashi revolutionized the stem cell field with their publication in Cell demonstrating the possibility of reprogramming somatic cells into pluripotent stem cells.

Since those findings, investigators have been working towards developing a relevant cardiomyocyte model from induced pluripotent stem cells (iPSC) for use in drug discovery, disease modeling, translation, cell therapy and cardiotoxicity. The model must simulate an adult cardiomyocyte and have the right structure, gene expression, contractility and metabolic function. Recent publications demonstrated that heart failure is a bioenergetic disease and metabolism drives cellular function: preceding structure, gene expression, and contractility.

In this seminar you will learn:

  1. The emerging role of bioenergetics to predict, monitor and track cell fate transitions of iPSC-derived cardiomyocytes
  2. How to apply Agilent Seahorse XF assays or soluble metabolic sensors along with BioTek’s Cytation to assess metabolic status after different approaches of maturation: biochemical, environmental manipulation and 3D models
  3. Publication examples of how metabolic assays are used with matured iPSC-derived cardiomyocytes in disease modeling, therapeutics, drug discovery, and more.
  4. Agilent’s offerings to support cardiovascular research: Seahorse XF Technology Metabolic Analysis, xCELLigence RTCA ePacer, BioTek Cytation Multi-mode Imager/Plate Reader, Novocyte Flow Cytometer, etc.

Speakers

Jestine Ho
Applications Scientist, West Coast, Cell Analysis
Agilent Technologies, Inc.

Jestine has been supporting Agilent Seahorse XF Technology as a Field Applications Scientist since 2010. Prior to joining Agilent Technologies, she worked at various biotechnology companies researching, developing, optimizing and commercializing novel applications and reagent kits. She developed cell-based assays and reagent kits for studying apoptosis pathway, tracking cell proliferation and viability and detecting cell cycle phase arrested.

Brian Kierce
Product Specialist for Seahorse XF
Agilent Technologies, Inc.

Prior to becoming a product specialist at Agilent in 2016 for the Seahorse division, Brian held technical sales and management positions for 12yrs, most of that time at Miltenyi Biotec, Inc.

Previously, Brian worked as a life science researcher in the areas of molecular biology and immunology at Genentech and Applied Biosystems.

Event Location

  • Webinar