PhD, Northwestern University, Chicago, IL, 2005
BS, Indiana University, Bloomington, IN 1999
Therapeutic uses of stem cells and disease modeling for spinal muscular atrophy, amyotrophic lateral sclerosis, Parkinson’s disease, and muscular dystrophy
My research interests are in the area of neurodegenerative diseases, both understanding the molecular basis for the disease progression and finding effective experimental therapies. My current research focuses on using induced pluripotent stem cells (iPSCs) derived from patient tissue to understand disease mechanisms and therapeutic intervention. One project in my lab is investigating the cell death processes involved in motor neuron loss in spinal muscular atrophy (SMA). We are using iPSCs derived from SMA patients to generate motor neurons and astrocytes to determine how the motor neurons are. My lab has found that astrocytes are dysfunctional in SMA, so we are investigating the mechanisms by which they contribute to disease pathology including alterations in secreted proteins and microRNAs. My lab also uses gene therapy and cell transplantation as potential therapeutic strategies for SMA. The second project is investigating the mechanisms underlying motor neuron loss in amyotrophic lateral sclerosis (ALS). We are using patient-specific iPSCs to assess protein aggregation in common and rare genetic forms of ALS. We incorporate gene editing techniques to create specific iPSCs for differentiation into motor neurons, astrocytes, and skeletal muscle. The third project is evaluating the effect of the G2019S LRRK2 mutation on mitochondrial trafficking and cytoskeletal structure in iPSC-derived dopamine neurons and sensory neurons. We have found the same mutation induces different consequences depending on the cell type being examined, so we are now using live cell imaging to determine mitochondrial and vesicular motility in an effort to correlate neuron-specific defects to disease phenotypes. Finally, we are examining the secretome and microRNA profile of dystrophin deficient iPSC-derived myocytes to identify novel therapeutic targets to treat muscular dystrophy.
(Logan S, Arzua T, Canfield SG, Seminary ER, Sison SL, Ebert AD, Bai X.) Compr Physiol. 2019 Mar 14;9(2):565-611.
(Sison SL, Vermilyea SC, Emborg ME, Ebert AD.) Curr Neurol Neurosci Rep. 2018 Oct 04;18(12):84.
(Santarriaga S, Haver HN, Kanack AJ, Fikejs AS, Sison SL, Egner JM, Bostrom JR, Seminary ER, Hill RB, Link BA, Ebert AD, Scaglione KM.) Mol Cell. 2018 07 19;71(2):216-228.e7.
(Sison SL, Ebert AD.) Aging (Albany NY). 2018 Apr 28;10(4):526-527.
(Gray KM, Kaifer KA, Baillat D, Wen Y, Bonacci TR, Ebert AD, Raimer AC, Spring AM, Have ST, Glascock JJ, Gupta K, Van Duyne GD, Emanuele MJ, Lamond AI, Wagner EJ, Lorson CL, Matera AG.) Mol Biol Cell. 2018 01 15;29(2):96-110.
(Seminary ER, Sison SL, Ebert AD.) Front Neurosci. 2018;12:86.
(Schwab AJ, Sison SL, Meade MR, Broniowska KA, Corbett JA, Ebert AD.) Stem Cell Reports. 2017 12 12;9(6):1839-1852.
(Sison SL, Patitucci TN, Seminary ER, Villalon E, Lorson CL, Ebert AD.) Hum Mol Genet. 2017 09 01;26(17):3409-3420.
(Afzal MZ, Reiter M, Gastonguay C, McGivern JV, Guan X, Ge ZD, Mack DL, Childers MK, Ebert AD, Strande JL.) J Cardiovasc Pharmacol Ther. 2016 11;21(6):549-562.
(Osman EY, Washington CW 3rd, Kaifer KA, Mazzasette C, Patitucci TN, Florea KM, Simon ME, Ko CP, Ebert AD, Lorson CL.) Mol Ther. 2016 09;24(9):1592-601.
(Bigley TM, McGivern JV, Ebert AD, Terhune SS.) Antiviral Res. 2016 May;129:67-73.
(Schwab AJ, Ebert AD.) Stem Cell Reports. 2015 Dec 08;5(6):1039-1052.
Jered McGivern, PhD, Postdoctoral Fellow, January 2011-July 2014, is now an Assistant Professor in the Department of Biochemistry at Lakeland College, Sheboygan, WI.
Teresa Patitucci, PhD, Graduate Student, 2016 is now Assistant Professor in Anatomy, Medical College of Wisconsin Regional Campus, Wausau, WI
Andrew Schwab, PhD, Graduate Student, 2016 is now a Postdoctoral Fellow at National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, NC