Brian Smith, PhD

Associate Professor

Locations

Biochemistry
BSB 356

Contact Information

Education

PhD, University of Wisconsin - Madison, 2008
BS, University of Illinois at Urbana-Champaign, 2003

Please see the Smith lab website for more information

Biography

Dr. Smith received his Bachelor of Science in Chemistry from the University of Illinois at Urbana-Champaign in 2003. He then received his PhD in Chemistry from the University of Wisconsin-Madison in 2008, where his research focused on the chemical mechanisms of sirtuin protein lysine deacetylases. During his graduate career he spent a brief period at Genentech in the Department of Medicinal Chemistry where he synthesized drugs that target the Inhibitor of Apoptosis family of proteins. From 2009-2014, Dr. Smith performed postdoctoral studies on nitric oxide synthases and cysteine S-nitrosation at the University of California, Berkeley and the Scripps Research Institute in La Jolla. During his postdoctoral studies Dr. Smith was awarded an NIH Ruth L. Kirschstein National Research Service Award. Dr. Smith joined the faculty at the Medical College of Wisconsin in 2014.

                
                    Research Experience
                

                    Alkylating Agents
Amino Acids, Peptides, and Proteins
Binding Sites
Biochemistry
Catalysis
Deuterium Exchange Measurement
Enzyme Activators
Enzyme Inhibitors
Enzymes and Coenzymes
Histone Code
Histone Deacetylases
Mass Spectrometry

            

                
                    Methodologies and Techniques
                

                    Biochemistry
Biological Assay
chemical synthesis
Chromatography, Affinity
Electrophoresis
enzymology
Mass Spectrometry
Models, Chemical
Models, Molecular
Mutagenesis
Nuclear Magnetic Resonance, Biomolecular
Peptide Library

            

                
                    MCW Program / Core Facilities
                

                    Biochemistry
Redox Biology

            

Molecular docking-guided synthesis of NSAID-glucosamine bioconjugates and their evaluation as COX-1/COX-2 inhibitors with potentially reduced gastric toxicity.

(Jones Lipinski RA, Thillier Y, Morisseau C, Sebastiano CS Jr, Smith BC, Hall CD, Katritzky AR.) Chem Biol Drug Des. 2021 Jul;98(1):102-113 PMID: 33955172 PMCID: PMC8281356 05/07/2021
Molecular mechanics and dynamic simulations of well-known Kabuki syndrome-associated KDM6A variants reveal putative mechanisms of dysfunction.

(Chi YI, Stodola TJ, De Assuncao TM, Leverence EN, Tripathi S, Dsouza NR, Mathison AJ, Basel DG, Volkman BF, Smith BC, Lomberk G, Zimmermann MT, Urrutia R.) Orphanet J Rare Dis. 2021 02 05;16(1):66 PMID: 33546721 PMCID: PMC7866879 SCOPUS ID: 2-s2.0-85100579633 02/07/2021
Development of activity-based probes for the protein deacylase Sirt1.

(Goetz CJ, Sprague DJ, Smith BC.) Bioorg Chem. 2020 11;104:104232 PMID: 32911193 PMCID: PMC7686039 09/11/2020
Cysteine sulfenylation by CD36 signaling promotes arterial thrombosis in dyslipidemia.

(Yang M, Li W, Harberg C, Chen W, Yue H, Ferreira RB, Wynia-Smith SL, Carroll KS, Zielonka J, Flaumenhaft R, Silverstein RL, Smith BC.) Blood Adv. 2020 09 22;4(18):4494-4507 PMID: 32946569 PMCID: PMC7509873 SCOPUS ID: 2-s2.0-85092260584 09/19/2020
Human sirtuins are differentially sensitive to inhibition by nitrosating agents and other cysteine oxidants.

(Kalous KS, Wynia-Smith SL, Summers SB, Smith BC.) J Biol Chem. 2020 06 19;295(25):8524-8536 PMID: 32371394 PMCID: PMC7307208 05/07/2020
ICEKAT: an interactive online tool for calculating initial rates from continuous enzyme kinetic traces.

(Olp MD, Kalous KS, Smith BC.) BMC Bioinformatics. 2020 May 14;21(1):186 PMID: 32410570 PMCID: PMC7222511 05/16/2020
Validation and Characterization of Five Distinct Novel Inhibitors of Human Cytomegalovirus.

(Kapoor A, Ghosh AK, Forman M, Hu X, Ye W, Southall N, Marugan J, Keyes RF, Smith BC, Meyers DJ, Ferrer M, Arav-Boger R.) J Med Chem. 2020 04 23;63(8):3896-3907 PMID: 32191456 PMCID: PMC7386824 SCOPUS ID: 2-s2.0-85084027851 03/20/2020
Covalent-Fragment Screening of BRD4 Identifies a Ligandable Site Orthogonal to the Acetyl-Lysine Binding Sites.

(Olp MD, Sprague DJ, Goetz CJ, Kathman SG, Wynia-Smith SL, Shishodia S, Summers SB, Xu Z, Statsyuk AV, Smith BC.) ACS Chem Biol. 2020 04 17;15(4):1036-1049 PMID: 32149490 PMCID: PMC7271629 03/10/2020
Mitochondrial Metabolic Reprogramming by CD36 Signaling Drives Macrophage Inflammatory Responses.

(Chen Y, Yang M, Huang W, Chen W, Zhao Y, Schulte ML, Volberding P, Gerbec Z, Zimmermann MT, Zeighami A, Demos W, Zhang J, Knaack DA, Smith BC, Cui W, Malarkannan S, Sodhi K, Shapiro JI, Xie Z, Sahoo D, Silverstein RL.) Circ Res. 2019 12 06;125(12):1087-1102 PMID: 31625810 PMCID: PMC6921463 SCOPUS ID: 2-s2.0-85076330129 10/19/2019
Non-sedating benzodiazepines cause paralysis and tissue damage in the parasitic blood fluke Schistosoma mansoni.

(McCusker P, Mian MY, Li G, Olp MD, Tiruveedhula VVNPB, Rashid F, Golani LK, Verma RS, Smith BC, Cook JM, Chan JD.) PLoS Negl Trop Dis. 2019 11;13(11):e0007826 PMID: 31730614 PMCID: PMC6881066 11/16/2019
Comparative and integrative metabolomics reveal that S-nitrosation inhibits physiologically relevant metabolic enzymes.

(Bruegger JJ, Smith BC, Wynia-Smith SL, Marletta MA.) J Biol Chem. 2018 04 27;293(17):6282-6296 PMID: 29483187 PMCID: PMC5925818 02/28/2018
Calmodulin-induced Conformational Control and Allostery Underlying Neuronal Nitric Oxide Synthase Activation.

(Hanson QM, Carley JR, Gilbreath TJ, Smith BC, Underbakke ES.) J Mol Biol. 2018 03 30;430(7):935-947 PMID: 29458127 02/20/2018

Brian Smith, PhD

Brian Smith, PhD

Associate Professor

Locations

Contact Information

Education

Biography

Research Experience

Methodologies and Techniques

MCW Program / Core Facilities

Publications