Daniel M. Lipinski, DPhil | Assistant Professor

Daniel Lipinski, MSc, DPhil

Assistant Professor of Ophthalmology and Visual Sciences, Assistant Professor of Cell Biology, Neurobiology and Anatomy

Locations

Medical College of Wisconsin Eye Institute
925 N. 87th St. Room Room 722
Milwaukee WI, 53226

Contact Information

Education

BSc, Biology, Imperial College London, UK
MSc, Virology, Imperial College London, UK
DPhil, Ophthalmology, University of Oxford, UK

Research Interests

Ocular Gene Therapy Lab

Research Interests

Recombinant adeno-associated virus (rAAV)-mediated gene therapy
Diabetic retinopathy
Inherited retinal disease (e.g., Retinitis pigmentosa)
Age-related macular degeneration (AMD)

Research Team

Kristina J. Ertel, Graduate Student
Dwani D. Patel, Graduate Student
Hanmeng Zhang, Graduate Student
Emily Nettesheim, Research Technologist
Amira Pavlovich, Research Associate

Research Summary

The overarching theme of my research program is the development of broadly applicable gene-based therapeutics to preserve vision in neurodegenerative and vascular diseases affecting the retina. Toward this end, the Lipinski research group focuses on a number of key areas:

1) The development of recombinant adeno-associated virus (rAAV)-mediated gene therapies to prevent cone photoreceptor degeneration in end-stage retinitis pigmentosa.

2) Developing gene therapy approaches to target the retinal and choroidal vasculature in diabetic retinopathy and age-related macular degeneration.

3) Restoration of light sensitivity/vision in the totally degenerate retina.

Funding for this research: Department of Ophthalmology Special Endowment (PI: Lipinski DM)
Past grant support/funding: Juvenile Diabetes Research Foundation (JDRF) Innovative Grant (2014-2015, PI: Lipinski DM); Fulbright-Fight for Sight (UK) Research Scholarship (2013-2015, PI: Lipinski DM); Fight for Sight (UK) PhD Studentship (2009-2012, PI: MacLaren RE)

Presynaptic Expression of LRIT3 Transsynaptically Organizes the Postsynaptic Glutamate Signaling Complex Containing TRPM1.

(Hasan N, Pangeni G, Cobb CA, Ray TA, Nettesheim ER, Ertel KJ, Lipinski DM, McCall MA, Gregg RG.) Cell Rep. 2019 Jun 11;27(11):3107-3116.e3 PMID: 31189098 PMCID: PMC6628893 06/13/2019
Acid Ceramidase Deficiency in Mice Leads to Severe Ocular Pathology and Visual Impairment.

(Yu FPS, Sajdak BS, Sikora J, Salmon AE, Nagree MS, Gurka J, Kassem IS, Lipinski DM, Carroll J, Medin JA.) Am J Pathol. 2019 02;189(2):320-338 PMID: 30472209 PMCID: PMC6412726 SCOPUS ID: 2-s2.0-85060099082 11/26/2018
A Comparison of Inducible Gene Expression Platforms: Implications for Recombinant Adeno-Associated Virus (rAAV) Vector-Mediated Ocular Gene Therapy.

(Lipinski DM.) Adv Exp Med Biol. 2019;1185:79-83 PMID: 31884592 12/31/2019
A Destabilizing Domain Allows for Fast, Noninvasive, Conditional Control of Protein Abundance in the Mouse Eye - Implications for Ocular Gene Therapy.

(Datta S, Renwick M, Chau VQ, Zhang F, Nettesheim ER, Lipinski DM, Hulleman JD.) Invest Ophthalmol Vis Sci. 2018 10 01;59(12):4909-4920 PMID: 30347085 PMCID: PMC6181441 10/23/2018
Development of an inducible anti-VEGF rAAV gene therapy strategy for the treatment of wet AMD.

(Reid CA, Nettesheim ER, Connor TB, Lipinski DM.) Sci Rep. 2018 08 06;8(1):11763 PMID: 30082848 PMCID: PMC6079038 SCOPUS ID: 2-s2.0-85051247613 08/08/2018
Small and Micro-Scale Recombinant Adeno-Associated Virus Production and Purification for Ocular Gene Therapy Applications.

(Reid CA, Lipinski DM.) Methods Mol Biol. 2018;1715:19-31 PMID: 29188503 12/01/2017
Improvement of Photoreceptor Targeting via Intravitreal Delivery in Mouse and Human Retina Using Combinatory rAAV2 Capsid Mutant Vectors.

(Reid CA, Ertel KJ, Lipinski DM.) Invest Ophthalmol Vis Sci. 2017 12 01;58(14):6429-6439 PMID: 29260200 PMCID: PMC5736327 12/21/2017
miRNA-mediated post-transcriptional silencing of transgenes leads to increased adeno-associated viral vector yield and targeting specificity.

(Reid CA, Boye SL, Hauswirth WW, Lipinski DM.) Gene Ther. 2017 08;24(8):462-469 PMID: 28617420 06/16/2017
Single residue AAV capsid mutation improves transduction of photoreceptors in the Abca4 mouse and bipolar cells in the rd1 mouse and human retina ex vivo.

(De Silva SR, Charbel Issa P, Singh MS, Lipinski DM, Barnea-Cramer AO, Walker NJ, Barnard AR, Hankins MW, MacLaren RE.) Gene Ther. 2016 11;23(11):767-774 PMID: 27416076 PMCID: PMC5097463 11/04/2016
Systemic Vascular Transduction by Capsid Mutant Adeno-Associated Virus After Intravenous Injection.

(Lipinski DM, Reid CA, Boye SL, Peterson JJ, Qi X, Boye SE, Boulton ME, Hauswirth WW.) Hum Gene Ther. 2015 Nov;26(11):767-76 PMID: 26359319 PMCID: PMC4651034 09/12/2015
CNTF Gene Therapy Confers Lifelong Neuroprotection in a Mouse Model of Human Retinitis Pigmentosa.

(Lipinski DM, Barnard AR, Singh MS, Martin C, Lee EJ, Davies WIL, MacLaren RE.) Mol Ther. 2015 Aug;23(8):1308-1319 PMID: 25896245 PMCID: PMC4539573 04/22/2015
Vesicular stomatitis virus glycoprotein- and Venezuelan equine encephalitis virus-derived glycoprotein-pseudotyped lentivirus vectors differentially transduce corneal endothelium, trabecular meshwork, and human photoreceptors.

(Lipinski DM, Barnard AR, Charbel Issa P, Singh MS, De Silva SR, Trabalza A, Eleftheriadou I, Ellison SM, Mazarakis ND, MacLaren RE.) Hum Gene Ther. 2014 Jan;25(1):50-62 PMID: 24125177 10/16/2013

Ocular Gene Therapy

The Ocular Gene Therapy Laboratory (OGTL), founded by Daniel M. Lipinski, MSc, DPhil, and located in the Eye Institute, aims to develop broadly applicable gene-based therapeutics to prevent human blindness arising from neurodegenerative or vascular diseases affecting the retina. More information about this lab is available on the OGTL Website

OGT_Square_White

Daniel M Lipinski MSc DPhil

Daniel Lipinski, MSc, DPhil

Assistant Professor of Ophthalmology and Visual Sciences, Assistant Professor of Cell Biology, Neurobiology and Anatomy

Locations

Contact Information

Education

Research Interests

Ocular Gene Therapy Lab

Research Interests

Research Team

Research Summary

Publications

Ocular Gene Therapy