Cheryl L. Stucky, PhD
Marvin Wagner Professor; Director, Neuroscience Doctoral Program
- Cell Biology, Neurobiology & Anatomy
Postdoctoral, University of Würzburg, Würzburg, Germany and Max Delbrück Center for Molecular Medicine, Berlin, Germany
Dr. Stucky is the director of the Neuroscience Doctoral Program.
Interested in applying to the Neuroscience Doctoral Program?
Available Lab Positions
Postdoctoral Fellow Position to Study Pain and Touch (PDF)
Postdoctoral Fellow Position to study the cellular mechanisms that drive acute and chronic pain in sickle cell disease (an inherited red blood cell disorder), keratinocyte-sensory neuron signaling, and/or migraine headache.
Dr. Stucky was recently featured on Discovery Radio: Opioid Epidemic Update & Pain Research - discover what researchers are learning about chronic pain and pain treatment
Chronic pain affects approximately 100 million adults in the United States, costing around $635 billion and many patients are sub-optimally treated as a result of limited understanding of the mechanistic causes of the chronic pain. The Stucky Lab has made key contributions to the pain field’s understanding of how ion channels on pain-sensing neurons contribute to pain and touch sensation. We are known for the unique “skin-nerve” recording technique whereby sensory afferent responses from rodents are measured in their native skin environment. We were the first lab to demonstrate that the Transient Receptor Potential Ankyrin 1 (TRPA1) channel is essential for detection of painful mechanical stimuli in normal, non-injured skin by using parallel genetic deletion and pharmacological inhibition of the TRPA1 channel. This work was published in the Journal of Neuroscience and Molecular Pain in 2009. Since that time, numerous publications have emerged that further build upon this work, including a widely-cited manuscript demonstrating that TRPA1 is responsible for the mechanical sensitization of pain receptors after tissue inflammation (Lennertz et al., 2012, PLoS ONE), and therefore, can serve as a target for inhibiting pain in many common inflammatory disorders.
An exciting current direction in our lab is identifying the mechanisms underlying the role of chronic pain in damaged skin, by examining the bidirectional signaling between keratinocytes and sensory neurons in normal and tissue-injured skin. While sensory neurons have long been known to mediate touch and pain transduction, epidermal keratinocytes are the initial “first responders” to tactile stimuli. We are dissecting the cellular mechanisms by which keratinocytes communicate with sensory nerve terminals, and conversely, the mechanisms by which sensory neurons communicate and sensitize keratinocytes during tissue injury. We are using multiple complementary pharmacological and cutting edge site-selective genetic approaches, such as optogenetic silencing, CRISPR/Cas9 gene editing and “cell sniffer” assays to interrogate the mechanistic direction and molecules underlying keratinocyte to sensory neuron signaling in vivo.
Another major area of focus is on translational models of chronic pain including inflammation, nerve injury and diseases associated with devastating pain, particularly in areas of unmet medical need. For example, patients with sickle cell disease have severe pain during red cell sickling crises and develop chronic underlying pain; effective treatments for this pain are lacking. We have made key discoveries in mechanisms that underlie the severe pain in sickle cell disease by performing parallel studies in mouse models of sickle cell disease and concomitantly measuring pain in patients with sickle cell disease (Hillery et al., 2011, Blood; Brandow et al., 2013, American Journal of Hematology; Zappia et al., 2014, Pain). Sickle cell disease is of particular interest because 1) it has aspects of chronic as well as acute pain, 2) the pain develops naturally as part of the underlying disease and therefore, may serve as a model for other naturally-occurring types of chronic pain in humans, and 3) parallel studies in the animal models and in patients with sickle cell disease can be conducted by the same laboratory.
Support for these projects
R01 NS40538; R01 NS070711; R21 NS095627-01; Advancing a Healthier Wisconsin
(Farrell AT, Panepinto J, Carroll CP, Darbari DS, Desai AA, King AA, Adams RJ, Barber TD, Brandow AM, DeBaun MR, Donahue MJ, Gupta K, Hankins JS, Kameka M, Kirkham FJ, Luksenburg H, Miller S, Oneal PA, Rees DC, Setse R, Sheehan VA, Strouse J, Stucky CL, Werner EM, Wood JC, Zempsky WT.) Blood Adv. 2019 Dec 10;3(23):3982-4001 PMID: 31809538 PMCID: PMC6963237 SCOPUS ID: 2-s2.0-85076356716 12/07/2019
(Park SK, Gunaratne GS, Chulkov EG, Moehring F, McCusker P, Dosa PI, Chan JD, Stucky CL, Marchant JS.) J Biol Chem. 2019 Dec 06;294(49):18873-18880 PMID: 31653697 PMCID: PMC6901322 SCOPUS ID: 2-s2.0-85075816525 10/28/2019
(Sadler KE, Langer SN, Menzel AD, Moehring F, Erb AN, Brandow AM, Stucky CL.) Br J Haematol. 2019 Oct;187(2):246-260 PMID: 31247672 PMCID: PMC6786911 SCOPUS ID: 2-s2.0-85068153982 06/28/2019
(Sadler KE, Lewis TR, Waltz TB, Besharse JC, Stucky CL.) Pain Rep. 2019 Jul-Aug;4(4):e765 PMID: 31579856 PMCID: PMC6728004 10/04/2019
(Cowie AM, Menzel AD, OʼHara C, Lawlor MW, Stucky CL.) Pain. 2019 08;160(8):1794-1816 PMID: 31335648 PMCID: PMC6662742 SCOPUS ID: 2-s2.0-85069147648 07/25/2019
(Sadler KE, Stucky CL.) Blood. 2019 05 02;133(18):1924-1925 PMID: 31048303 SCOPUS ID: 2-s2.0-85065494775 05/03/2019
(Sadler KE, Stucky CL.) Neurosci Lett. 2019 02 16;694:184-191 PMID: 30508569 PMCID: PMC6389361 SCOPUS ID: 2-s2.0-85058162306 12/07/2018
(Cowie AM, Stucky CL.) Bio Protoc. 2019 Jan 20;9(2) PMID: 30820443 PMCID: PMC6390978 03/02/2019
(Donaldson LF, Stucky CL.) Current Opinion in Physiology. October 2019;11:iii-v SCOPUS ID: 2-s2.0-85076007755 10/01/2019
(Cowie AM, Dittel BN, Stucky CL.) Front Neurol. 2019;10:622 PMID: 31244767 PMCID: PMC6581722 06/28/2019
(Moehring F, Halder P, Seal RP, Stucky CL.) Neuron. 2018 10 24;100(2):349-360 PMID: 30359601 PMCID: PMC6708582 SCOPUS ID: 2-s2.0-85055049128 10/26/2018
Francie Moehring, Ashley Reynolds, Crystal O'Hara, Cheryl Stucky, Kate Sadler, Sarah Langer, Tony Menzel
Summer 2017 students
Melissa Stagg, Christina Mecca
Recent News in the Stucky Lab
American Pain Society Meeting 2019
The 2019 American Pain Society Meeting was held in Milwaukee. Dr. Kate Sadler, postdoctoral fellow in Dr. Cheryl Stucky’s lab chaired a session entitled “Translational Models for Investigation of Non-Opioid Based Pain Therapies for Sickle Cell Disease”. She and clinical collaborator Amanda Brandow, DO gave an integrated 30 minute talk that discussed neuropathic pain mechanisms and treatments in both patients and mice with sickle cell disease.
Other meeting highlights
Dr. Cheryl Stucky chaired a symposium at the Spring Pain pre-meeting. Stucky Lab members, Sarah Langer, Tony Menzel, Francie Moehring, PhD & Kate Sadler, PhD presented posters.
Congrats to Kate Sadler, PhD, who has been awarded an NINDS Ruth L. Kirchstein National Research Service Award (NRSA) for Training of Postdoctoral Fellows (F32). Kate’s proposal entitled “Fibroblast-to-neuron communication in muscle pain” will explore the extent and mechanisms through which muscle support cells communicate with sensory neurons to relay both painful and non-painful signals to the central nervous system. This unique award is presented to promising, early-stage postdoctoral fellows and will fund Kate’s position until January 2020.
Congrats to Kate Sadler, PhD who has been selected to participate in the 2018 North American Pain School. The meeting will be held in Quebec from June 24-28 and will bring together basic and clinical pain trainees from the US and Canada. Read more on the North American Pain School site.
Congrats to Cheryl Stucky, PhD who has been selected to participate in the Mayday Pain & Society Fellowship program as one of 12 national experts in pain science and care.
Congratulations to Ashley Cowie, graduate student in the Stucky Lab. The National Institutes of Health (NIH) has invited Ashley Cowie to present her research at their poster session at the 13th Annual Symposium on Advances in Pain Research to be held on May 31 and June 1, 2018 at the NIH Campus in Bethesda, MD. The intent of the symposium is to present new and exciting advances in pain research, featuring work done through NIH support!
Congratulations to Francie Moehring, graduate student in the Stucky Lab. Francie recently won the best Early Career Basic Science Poster at the American Pain Society Meeting.
The following manuscript, Neuropathic pain in a new Fabry Disease rat model has been accepted for publication by JCI Insight.
Co-corresponding authors are Dr. Cheryl Stucky, Dr. Nancy Dahms & Dr. Michael Tiemeyer.
The publication demonstrates that a novel rat model of Fabry disease mimics many of the patient pathologies such as prominent mechanical hypersensitivity and extensive accumulations of lipids in tissues such as dorsal root ganglia. Sensory neurons from these animals were also sensitized to mechanical probing, and TRPA1 antagonism reversed the behavioral mechanical sensitization, pointing towards TRPA1 as a novel target to treat the pain experienced by patients with Fabry disease.
James J. Miller1§, Kazuhiro Aoki2§, Francie Moehring3§, Carly A. Murphy1, Crystal L. O’Hara3, Michael Tiemeyer2*, Cheryl L. Stucky3*, Nancy M. Dahms1*. Neuropathic pain in a new Fabry Disease rat model JCI Insight. in press.
Congratulations to Francie Moehring, graduate student in the Stucky Lab. Francie is first author on a new publication in the journal eLife entitled: Keratinocytes mediate innocuous and noxious touch via ATP-P2X4 signaling.
The publication reveals the signaling mechanism between skin keratinocytes and sensory neurons, which is essential for our normal sensitivity to gentle and painful touch in the skin. Francie's work has also been featured in Technology Networks entitled: Touching: Skin cell to nerve cell communication uncovered
Ashley Cowie, graduate student in the Stucky Lab, presented her work in a nanosymposium at the Society for Neuroscience meeting in Washington DC in November 2017.
Her research has been featured on the Pain Research Forum.
The Stucky Lab recently attended a team based learning activity off campus at Escape Milwaukee. They solved "Mr. K's Lethal Injection" with 9 minutes to spare! This was quite impressive to the organizer since this was the fastest they have seen any group escape!
Marie Barabas, PhD
Ashley Cowie, PhD
Sheldon Garrison, PhD
Josh Glazer, MD
Patrick (Pat) Kerstein, PhD
Richard C. Lennertz III, MD, PhD
Daniel Vilceanu, MD, PhD
Andy Weyer, PT, DPT, PhD
Katherine Zappia, MD, PhD
Awards, interviews & articles
- North American Pain School 2019: A Conversation With Visiting Faculty Member Cheryl Stucky
- Javits Neuroscience Award (PDF)
- The Quest to Ease the Pain of Sickle Cell Disease article
- Women’s History Month
- International Innovation (PDF)
- Standing Ovation Award for 2008 from MCW Medical Students for teaching Medical Neuroscience
- Bethel College Young Alumni Award for 2004
- John C. Liebeskind Early Career Scholar Award for 2002 for outstanding accomplishments in pain scholarship