Marquette-Medical College of Wisconsin NEIMO Lab Research in Optogenetics

Neural Engineering, Interfacing, Modulation and Optimization Lab Research

Research at the Neural Engineering, Interfacing, Modulation and Optimization Laboratory at the Marquette University and Medical College of Wisconsin Joint Department of Biomedical Engineering is interested in advancing viral gene therapy techniques in the peripheral nervous system that allow optical stimulation and restoration of paralyzed muscle activity, as well as using multi-modal brain recording and stimulation technologies to both advance neuroprosthetic system capabilities and build a better understanding of the underlying relationships between different scales of recorded neural signals. The research interests of this lab are fueled by a desire to fulfill a complementary circle of basic neuroscience investigation and translational neural engineering application in which discovery in one area opens up new avenues for investigation or advancement in the other and vice versa.

Peripheral Motor Optogenetics/Gene Therapy

About

The NEIMO Lab is working to develop techniques that enhance and prolong viral transduction of the peripheral motor system with the initial goal of robustly expressing light-sensitive ion channels (i.e., opsins) in motor nerves, allowing researchers to optically stimulate muscle activity as an alternative to electrical stimulation for reanimation of limbs paralyzed by conditions such as spinal cord injury. These experiments generally begin with injection of an opsin+fluorescent marker expressing virus—such as adeno-associated virus (AAV) vector or replication deficient Herpes Simplex Viruses—into a targeted muscle or nerve, followed by an incubation period to allow the virus to express its gene products, and finally periodic evaluation of opsin expression along the targeted nerve using a color-specific laser or LED light source. While optically stimulating the nerve, electromyographic (EMG) activity and muscle forces from the target muscle are recorded.

Representative Publication

Williams JJ, Watson AM, Vazquez AL, Schwartz AB. Viral-Mediated Optogenetic Stimulation of Peripheral Motor Nerves in Non-Human PrimatesFront Neurosci. 2019;13:759 PMID: 31417342 PMCID: PMC6684788 08/17/2019

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In Vivo light-induced activation of neural circuitry in transgenic rodent

Light activation of neural circuitry in transgenic rodent

 

 Multimodal, Optogenetic Brain-Computer Interfaces

About

The NEIMO Lab is interested in brain recording and stimulation modalities for advancing brain-computer interface (BCI) technology. A number of cortical signal recording modalities have been investigated as control signals for BCI systems—including single-unit or multi-unit activity (SUA/MUA), LFPs, ECoG, and EEG—each measuring a different scale of neural activity. Researchers have typically focused on the use of one modality independently of other possible signals for BCI use, emphasizing that signal’s strengths over the drawbacks of others while ignoring potential synergistic applications with other modalities; however, recent research efforts in the NEIMO Lab suggest that different signal modalities may be better suited for different components of a BCI framework than others, such as decoding finely graded movement parameters (e.g., SUAs) or deciphering attentional and task states (ECoG/EEG). In addition, the fundamental relationships between these signal modalities are not well understood. Investigators at the NEIMO Lab are looking to utilize multi-scale BCI paradigms as well as optogenetics to interrogate the fundamental relationships between these signal modalities.

Synapse

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In vivo neuronal activity captured via micrelectrode and imaged using Tucker-Davis Technologies' Synapse software