Joseph Besharse, PhD, FARVO
Marjorie & Joseph Heil Professor in Ophthalmology Research Director, the Eye Institute
- Medical College of Wisconsin Eye Institute
- 925 N. 87th St. Room 726
Milwaukee, WI 53226
MA, Southern Illinois University at Carbondale, Zoology
PhD, Southern Illinois University at Carbondale, Zoology and Physiology
Postdoctoral Fellow, College of Physicians and Surgeons, Columbia University, New York
Honors and Awards
Besharse Distinguished Service Award MCW 2015
Besharse MCW Vitality Award
Besharse Dean's Senior Leader Spotlight 2014
- Amino Acid Sequence
- Antibodies, Monoclonal
- Arylamine N-Acetyltransferase
- Biological Clocks
- Blotting, Western
- Cell Membrane
- Cells, Cultured
- Circadian Rhythm
- CLOCK Proteins
- Chair, Department of Cell Biology, Neurobiology and Anatomy, 1997-2016
- Director of Interdisciplinary Graduate Program in Biomedical Sciences, 2010-2014
- Director of Research, Eye Institute, 2015-present
- Cell biology of photoreceptor membrane turnover
- Cell biology of the retinal pigment epithelium
- Circadian biology of the retina
- Cilia, ciliogenesis and ciliopathies
- Photoreceptor degenerative diseases and macular degeneration
Funding for this research:
NIH/NEI R01 EY03222-31-36, J C Besharse (PI), The Visual Cell-Pigment Cell Interface and Disc Turnover
This grant is directed at the mechanisms involved in photoreceptor outer segment turnover.
NIH/NEI T32 EY014537-11-15, J C Besharse (PI), Research Training Program in Vision Science
This is a training grant in vision science for a group of senior and mid-level investigators, with active and competitive research programs funded through the National Eye Institute. The objective is to prepare trainees at the pre-doctoral level for research careers in ocular and vision research.
(Sadler KE, Lewis TR, Waltz TB, Besharse JC, Stucky CL.) Pain Rep. 2019 Jul-Aug;4(4):e765 PMID: 31579856 PMCID: PMC6728004 10/04/2019
(Spencer WJ, Ding JD, Lewis TR, Yu C, Phan S, Pearring JN, Kim KY, Thor A, Mathew R, Kalnitsky J, Hao Y, Travis AM, Biswas SK, Lo WK, Besharse JC, Ellisman MH, Saban DR, Burns ME, Arshavsky VY.) Proc Natl Acad Sci U S A. 2019 Jun 25;116(26):13087-13096 PMID: 31189593 PMCID: PMC6601265 SCOPUS ID: 2-s2.0-85068112749 06/14/2019
(Han DP, Skumatz C, Besharse JC, Kassem IS.) J Ocul Pharmacol Ther. 2019 Jun;35(5):278-282 PMID: 30916605 PMCID: PMC6588124 SCOPUS ID: 2-s2.0-85067020226 03/28/2019
(Lewis TR, Kundinger SR, Link BA, Insinna C, Besharse JC.) BMC Cell Biol. 2018 11 20;19(1):25 PMID: 30458707 PMCID: PMC6245759 SCOPUS ID: 2-s2.0-85056802940 11/22/2018
(Lewis TR, Zareba M, Link BA, Besharse JC.) Mol Biol Cell. 2018 01 15;29(2):180-190 PMID: 29142075 PMCID: PMC5909930 SCOPUS ID: 2-s2.0-85040967232 11/17/2017
(Lewis TR, Kundinger SR, Pavlovich AL, Bostrom JR, Link BA, Besharse JC.) Dev Biol. 2017 05 15;425(2):176-190 PMID: 28341548 PMCID: PMC5558849 SCOPUS ID: 2-s2.0-85016498928 03/28/2017
(Collery RF, Volberding PJ, Bostrom JR, Link BA, Besharse JC.) Invest Ophthalmol Vis Sci. 2016 12 01;57(15):6805-6814 PMID: 28002843 PMCID: PMC5215506 SCOPUS ID: 2-s2.0-85007349845 12/22/2016
(Besharse JC, McMahon DG.) J Biol Rhythms. 2016 06;31(3):223-43 PMID: 27095816 PMCID: PMC5479307 SCOPUS ID: 2-s2.0-84966564991 04/21/2016
(Miesfeld JB, Gestri G, Clark BS, Flinn MA, Poole RJ, Bader JR, Besharse JC, Wilson SW, Link BA.) Development. 2015 Sep 01;142(17):3021-32 PMID: 26209646 PMCID: PMC4582179 SCOPUS ID: 2-s2.0-84940754780 07/26/2015
(Fogerty J, Besharse JC.) Adv Exp Med Biol. 2014;801:355-63 PMID: 24664718 PMCID: PMC4273561 SCOPUS ID: 2-s2.0-84904807031 03/26/2014
(Wong-Riley MT, Besharse JC.) Biomol Concepts. 2012 Jun 01;3(3):267-282 PMID: 23762210 PMCID: PMC3677786 06/14/2013
(Lewis, TRrnKundinger, SRrnLink, BA rnBesharse, JC.) bioRxiv. bioRxiv 351122; doi: https://doi.org/10.1101/351122 06/20/2018
Joseph Besharse Lab
The current major focus of the laboratory is the role of ciliogenesis defects and circadian clocks in retinal diseases. This includes a particular focus on hereditary photoreceptor degeneration, macular degeneration and ciliopathies.
Pictured left to right: Amy Ludwig-Kubinski, Amira Pavlovich, Sean Kundinger, Tylor Lewis and Joseph Besharse
Positions in the lab
Please email Dr. Joseph Besharse for information about current positions.
“All of your organs have clocks — your liver, your heart, your kidneys all have their own internal clocks. And the big secret is how you mesh them all together and synchronize them so they work together instead of against each other."
|Read more about the Besharse Lab current research in the two sections below: Circadian Clocks and Trafficking of Phototransduction Components in Photoreceptors.|
Figure 1: Diagram illustrating the relationship of the central clockwork (left) and clock regulated genes (right). Only the Per/Cry loop of the clock is illustrated. Clock regulated genes are downstream of the clock and involved in circadian function within cells.
Trafficking of Phototransduction Components in Photoreceptors
Turnover of photosensitive membrane throughout the life of a photoreceptor depends on maintenance of a delicate balance between photosensitive membrane assembly and degradation. These events are controlled by circadian clocks (see above). Both protein and lipid components are synthesized in the cell body and transported vectorially to the region of the sensory cilium where phototransduction organelle is assembled. The cilium is important in the transport of macromolecules from sites of synthesis in the cell body to the region of membrane assembly and in the morphogenesis of flattened discs.
Our current work is directed at a model called intraflagellar transport (IFT) in which microtubule based motors (dynein and kinesin) move protein complexes along microtubule tracks into and out of the cilium (see Figure 2). The IFT model is thought to apply to all motile and sensory cilium structures among the eukaryotes; IFT proteins are required for cilium assembly and are found widely in both motile and sensory cilia.
Figure 2. The components of intraflagellar transport as revealed in the green alga, Chlamydomonas.
Recent work in mice carrying mutations in genes encoding the kinesin II motor and the IFT complex protein, IFT88, demonstrate that this pathway is required for assembly of the phototransduction system in photoreceptors (see Figure 3).
Research in this laboratory is directed at organization of the IFT protein complex and its physical interaction with both cell specific cargo and with microtubule based motor proteins.
Figure 3. A model for intraflagellar transport in photoreceptors based on work of Pazour, et al., 2002.
The 2016 ARVO highlight for MCW was the Proctor Medal lecture, given on May 2 by Joseph C. Besharse, PhD. The Proctor Medal, one of ARVO’s most prestigious honors, recognizes excellence in the basic or clinical sciences as applied to ophthalmology.
Symposium in honor of Joe Besharse's Birthday, "Sensory Biology: Photoreceptors, Clocks & Cilia"
Wednesday, April 16, 2014