Postdoctoral Fellow, Physical Chemistry, Medical College of Wisconsin, Milwaukee, WI, 1972-1976
PhD, Physical Chemistry, Iowa State University, Ames, Iowa, 1971
BS, Chemistry, University of Wisconsin-Madison, Madison, WI, 1965
My research interests involve the use of EPR spectroscopy to determine the ligation of metals (primarily copper) in proteins and complex biological systems. Current studies can be divided into two parts:
1. Collaborations with investigators interested in multifrequency EPR and metals.
2. More-involved studies with cupric ion bound to proteins where much more time is spent obtaining EPR samples.
Currently, I use multifrequency EPR to obtain EPR signals in three-copper mixed-valence model complexes (Justin Walensky, University of Missouri) and four-copper model complexes (Neal Mankad, University of Illinois-Chicago). This work is a continuation of studies on the two-copper mixed-valence site in cytochrome c oxidase done with Peter Kroneck (University of Konstanz, Germany).
In past studies, I used low-frequency EPR technology uniquely suited for prion research. I started using the loop-gap resonator in 1982 when it was developed by James S. Hyde (MCW) and Wojciech Froncisz (Jagiellonian University). I provided EPR analysis of the cupric sites in prions in collaboration with Glenn Millhauser (University of California, Santa Cruz). These studies aimed at identifying the molecular structure and function of the prion protein in its normal and abnormal states, a fundamental step toward developing treatments for prion diseases.
I mentored Hua (Julie) Yuan, a graduate student (1998), and Sebastiao Lemos, a visiting professor from the Universidade de Brasilia, on a project studying EPR-detectable copper in particulate methane monooxygenase (pMMO). Interest in pMMO is high due to a detoxification reaction for trichloroethylene, a priority pollutant. Methanotrophic bacteria use methane as the sole source of carbon and energy. pMMO oxidizes methane to methanol. I collaborated with Mary Lynn Perille Collins (University of Wisconsin-Milwaukee), Alan DiSpirito (Iowa State University), and students of Amy Rosenzweig (Northwestern University) on EPR projects related to this work. It is still to be determined whether pMMO has a new type of copper center.
Additional studies involved the detection of an iron-sulfur center in aconitase (William E. Walden, University of Illinois-Chicago), detection of the iron-sulfur center in Rieske protein (Toivo Kallas, University of Wisconsin-Oshkosh), detection of the tyrosyl radical in ribonucleotide reductase with several investigators; detection of metal complexes of known antitumor agents including bleomycin (David H. Petering, University of Wisconsin-Milwaukee), detection of the dimanganese site in gamma glutamylcysteine synthetase (Owen Griffith, MCW), and detection of chromium in the context of human lung toxicity (Charles Myers, MCW).
(Antholine WE, Vasquez-Vivar J, Quirk BJ, Whelan HT, Wu PK, Park JI, Myers CR.) Int J Mol Sci. 2019 Mar 06;20(5).
(Antholine WE, Zhang S, Gonzales J, Newman N.) Int J Mol Sci. 2018 Nov 09;19(11).
(Chitambar CR, Al-Gizawiy MM, Alhajala HS, Pechman KR, Wereley JP, Wujek R, Clark PA, Kuo JS, Antholine WE, Schmainda KM.) Mol Cancer Ther. 2018 06;17(6):1240-1250.
(Johnson BJ, Antholine WE, Lindeman SV, Graham MJ, Mankad NP.) J Am Chem Soc. 2016 Oct 12;138(40):13107-13110.
(Lane AC, Barnes CL, Antholine WE, Wang D, Fiedler AT, Walensky JR.) Inorg Chem. 2015 Sep 08;54(17):8509-17.
(Johnson BJ, Antholine WE, Lindeman SV, Mankad NP.) Chem Commun (Camb). 2015 Jul 28;51(59):11860-3.
(Xu J, Eriksson SE, Cebula M, Sandalova T, Hedström E, Pader I, Cheng Q, Myers CR, Antholine WE, Nagy P, Hellman U, Selivanova G, Lindqvist Y, Arnér ES.) Cell Death Dis. 2015 Jan 22;6:e1616.
(Vollmer MV, Machan CW, Clark ML, Antholine WE, Agarwal J, Schaefer HF 3rd, Kubiak CP, Walensky JR.) Organometallics. 2014 Sep 09;34(1):3-12.
(Myers JM, Cheng Q, Antholine WE, Kalyanaraman B, Filipovska A, Arnér ES, Myers CR.) Free Radic Biol Med. 2013 Jul;60:183-94.
(Hickok JR, Vasudevan D, Antholine WE, Thomas DD.) J Biol Chem. 2013 May 31;288(22):16004-15.
(Chitambar CR, Antholine WE.) Antioxid Redox Signal. 2013 Mar 10;18(8):956-72.
(Yin JJ, Fu PP, Lutterodt H, Zhou YT, Antholine WE, Wamer W.) J Agric Food Chem. 2012 Mar 14;60(10):2554-61.