
Candice S. Klug, PhD
James S. Hyde Professor of Biophysics; Director, National Biomedical EPR Center; Program Director, Biophysics Graduate Program
Locations
- Biophysics
MFRC 2026
Contact Information
Education
PhD, Biophysics, Medical College of Wisconsin, Milwaukee, WI, 1999
BS, Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 1994
Biography
Methodologies and Techniques
- Adenosine Triphosphatases
- Amino Acid Motifs
- ArnT
- Arrestin
- Arrestins
- ATP-Binding Cassette Transporters
- Bacterial Proteins
- electron paramagnetic resonance spectroscopy
- Electron Spin Resonance Spectroscopy
- Escherichia coli Proteins
- Lipid A
- Lipopolysaccharides
Leadership Positions
- Acting Director, IDP Program in Biomedical Sciences, 2019-2020
- Associate Director, National Biomedical EPR Center, 2014-2016
- Chair, Graduate School Curriculum and Programs Committee, 2010-2013
- Chair, Women in Science Advisory Committee, 2011 - 2016
- Chair, Women's Faculty Council, 2006-2007
- Director, National Biomedical EPR Center, 2016 - present
- President, MCW Faculty Council, 2016-2017
- Program Director, Biophysics Graduate Program, 2013 - present
- Training Program Director, National Biomedical EPR Center, 2003-present
MCW Program / Core Facilities
- Biophysics Graduate Program
- Interdisciplinary Program in the Biomedical Sciences
- National Biomedical EPR Center
Research Interests
Lpt Protein-Mediated Transport of LPS is an R01 award from the National Institute of General Medical Sciences. The goal of this project is to gain insights into the mechanism of LPS transport across the periplasm of Gram-negative bacteria using site-directed spin labeling EPR spectroscopy, complementary biophysical approaches and in vivo growth assays to enable rational antibiotic drug design.
Conformation and Functional Dynamics of a Bacterial PASTA Kinase is a multi-PI (with Dr. Christopher J. Kristich) R01 award from the National Institute of General Medical Sciences. The goal of this grant is to study the conformation and orientation of the intact IreK PASTA module in solution and in full-length IreK embedded in a membrane and elucidate the dynamic changes in the kinase domain associated with kinase activation using a complementary combination of in vivo functional analyses and biophysical approaches.
Lab Members
Malory Mueller, Research Technologist I
Kathryn Schultz, Research Scientist I
Publications
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(Chen Q, Zhuo Y, Sharma P, Perez I, Francis DJ, Chakravarthy S, Vishnivetskiy SA, Berndt S, Hanson SM, Zhan X, Brooks EK, Altenbach C, Hubbell WL, Klug CS, Iverson TM, Gurevich VV.) J Mol Biol. 2021 Feb 19;433(4):166790 PMID: 33387531 PMCID: PMC7870585 01/03/2021
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A non-GPCR-binding partner interacts with a novel surface on β-arrestin1 to mediate GPCR signaling.
(Zhuo Y, Gurevich VV, Vishnivetskiy SA, Klug CS, Marchese A.) J Biol Chem. 2020 10 09;295(41):14111-14124 PMID: 32753481 PMCID: PMC7549033 SCOPUS ID: 2-s2.0-85092803357 08/06/2020
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Rutile dielectric loop-gap resonator for X-band EPR spectroscopy of small aqueous samples.
(Mett RR, Sidabras JW, Anderson JR, Klug CS, Hyde JS.) J Magn Reson. 2019 10;307:106585 PMID: 31499469 PMCID: PMC6948142 SCOPUS ID: 2-s2.0-85071859156 09/10/2019
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(Schultz KM, Fischer MA, Noey EL, Klug CS.) Protein Sci. 2018 08;27(8):1407-1417 PMID: 29672978 PMCID: PMC6153404 SCOPUS ID: 2-s2.0-85047394661 04/20/2018
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Characterization of and lipopolysaccharide binding to the E. coli LptC protein dimer.
(Schultz KM, Klug CS.) Protein Sci. 2018 02;27(2):381-389 PMID: 29024084 PMCID: PMC5775163 SCOPUS ID: 2-s2.0-85040841909 10/13/2017
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(Schultz KM, Klug CS.) Appl Magn Reson. 2017 Dec;48(11-12):1341-1353 PMID: 29332998 PMCID: PMC5761346 01/16/2018
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Structural basis of arrestin-3 activation and signaling.
(Chen Q, Perry NA, Vishnivetskiy SA, Berndt S, Gilbert NC, Zhuo Y, Singh PK, Tholen J, Ohi MD, Gurevich EV, Brautigam CA, Klug CS, Gurevich VV, Iverson TM.) Nat Commun. 2017 11 10;8(1):1427 PMID: 29127291 PMCID: PMC5681653 SCOPUS ID: 2-s2.0-85033606940 11/12/2017
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Lipopolysaccharide binding to the periplasmic protein LptA.
(Schultz KM, Lundquist TJ, Klug CS.) Protein Sci. 2017 Aug;26(8):1517-1523 PMID: 28419595 PMCID: PMC5521551 SCOPUS ID: 2-s2.0-85018394516 04/19/2017
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Overview of arrestin mediated signaling with receptors and non-receptor binding partners
(Zhuo Y, Klug CS.) The Structural Basis of Arrestin Functions. 24 May 2017:19-29 SCOPUS ID: 2-s2.0-85034009393 05/24/2017
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GPCR footprint on arrestins and manipulation of receptor specificity
(Vishnivetskiy SA, Hubbell WL, Klug CS, Gurevich VV.) The Structural Basis of Arrestin Functions. 24 May 2017:133-140 SCOPUS ID: 2-s2.0-85033979110 05/24/2017
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Active conformations of arrestins: Expected and unexpected changes
(Bandyopadhyay A, Zhuo Y, Hubbell WL, Klug CS, Gurevich VV, Ernst OP.) The Structural Basis of Arrestin Functions. 24 May 2017:159-173 SCOPUS ID: 2-s2.0-85034008570 05/24/2017
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Directed evolution provides insight into conformational substrate sampling by SrtA.
(Suliman M, Santosh V, Seegar TCM, Dalton AC, Schultz KM, Klug CS, Barton WA.) PLoS One. 2017;12(8):e0184271 PMID: 28859178 PMCID: PMC5578623 SCOPUS ID: 2-s2.0-85028825732 09/01/2017