
Candice S. Klug, PhD
James S. Hyde Professor of Biophysics; Director, National Biomedical EPR Center; Program Director, Biophysics Graduate Program
Locations
- Biophysics
MFRC 2026
Contact Information
Education
PhD, Biophysics, Medical College of Wisconsin, Milwaukee, WI, 1999
BS, Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 1994
Biography
Research Interests
My lab studies the functional dynamics of both soluble and membrane proteins, with a specific focus on bacterial proteins involved in lipid transport using electron paramagnetic resonance (EPR) spectroscopy, as well as EPR technology development.
Lpt Protein-Mediated Transport of LPS is an R01 award from the National Institute of General Medical Sciences. The goal of this project is to gain insights into the mechanism of LPS transport across the periplasm of Gram-negative bacteria using site-directed spin labeling EPR spectroscopy, complementary biophysical approaches and in vivo growth assays to enable rational antibiotic drug design.
Conformation and Functional Dynamics of a Bacterial PASTA Kinase is a multi-PI (with Dr. Christopher J. Kristich) R01 award from the National Institute of General Medical Sciences. The goal of this grant is to study the conformation and orientation of the intact IreK PASTA module in solution and in full-length IreK embedded in a membrane and elucidate the dynamic changes in the kinase domain associated with kinase activation using a complementary combination of in vivo functional analyses and biophysical approaches.
Development of High-Throughput, High-Sensitivity EPR Sample Handling Capabilities for Biomedical Research is a multi-PI (with Dr. Michael Lerch) R01 award from the National Institute of General Medical Sciences. The goal of this grant is to develop two innovative EPR spectrometer technologies with outstanding sample sensitivity that are easy to use and widely available to the scientific community.
Lab Members
Nicholas Cina, Graduate Student
Kathryn Schultz, Research Scientist I
Publications
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(VanZeeland NE, Schultz KM, Klug CS, Kristich CJ.) J Mol Biol. 2023 Sep 15;435(18):168216 PMID: 37517789 PMCID: PMC10528945 SCOPUS ID: 2-s2.0-85166951228 07/31/2023
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(Schultz KM, Schneider JR, Fischer MA, Cina NP, Riegert MO, Frank DW, Klug CS.) Protein Sci. 2023 Aug;32(8):e4724 PMID: 37417889 PMCID: PMC10360375 SCOPUS ID: 2-s2.0-85165512022 07/07/2023
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Conformational changes in the activation loop of a bacterial PASTA kinase.
(Bluma MS, Schultz KM, Kristich CJ, Klug CS.) Protein Sci. 2023 Jul;32(7):e4697 PMID: 37312631 PMCID: PMC10303680 SCOPUS ID: 2-s2.0-85163810164 06/14/2023
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(Cina NP, Klug CS.) Applied Magnetic Resonance. 2023 SCOPUS ID: 2-s2.0-85166977110 01/01/2023
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The Two Non-Visual Arrestins Engage ERK2 Differently.
(Perry-Hauser NA, Hopkins JB, Zhuo Y, Zheng C, Perez I, Schultz KM, Vishnivetskiy SA, Kaya AI, Sharma P, Dalby KN, Chung KY, Klug CS, Gurevich VV, Iverson TM.) J Mol Biol. 2022 Apr 15;434(7):167465 PMID: 35077767 PMCID: PMC8977243 SCOPUS ID: 2-s2.0-85124130291 01/26/2022
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Use of Site-Directed Spin Labeling EPR Spectroscopy to Study Protein-LPS Interactions.
(Schultz KM, Klug CS.) Methods Mol Biol. 2022;2548:83-96 PMID: 36151493 SCOPUS ID: 2-s2.0-85138460647 09/24/2022
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(Chen Q, Zhuo Y, Sharma P, Perez I, Francis DJ, Chakravarthy S, Vishnivetskiy SA, Berndt S, Hanson SM, Zhan X, Brooks EK, Altenbach C, Hubbell WL, Klug CS, Iverson TM, Gurevich VV.) J Mol Biol. 2021 Feb 19;433(4):166790 PMID: 33387531 PMCID: PMC7870585 SCOPUS ID: 2-s2.0-85100159529 01/03/2021
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Applications of nitroxide spin labels to structural biology
(Klug, C.S., Lerch, M.T., Feix, J.B. .) Nitroxides: Synthesis, Properties, and Applications. Klug, C.S., Lerch, M.T., Feix, J.B. (2021) Applications of nitroxide spin labels to structural biology in: Nitroxides: Synthesis, Properties, and Applications. (Ouari, O., Gigmes, D., eds.) Royal Society of Chemistry, pp. 392-419. 05/14/2021
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A non-GPCR-binding partner interacts with a novel surface on β-arrestin1 to mediate GPCR signaling.
(Zhuo Y, Gurevich VV, Vishnivetskiy SA, Klug CS, Marchese A.) J Biol Chem. 2020 Oct 09;295(41):14111-14124 PMID: 32753481 PMCID: PMC7549033 SCOPUS ID: 2-s2.0-85092803357 08/06/2020
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Rutile dielectric loop-gap resonator for X-band EPR spectroscopy of small aqueous samples.
(Mett RR, Sidabras JW, Anderson JR, Klug CS, Hyde JS.) J Magn Reson. 2019 Oct;307:106585 PMID: 31499469 PMCID: PMC6948142 SCOPUS ID: 2-s2.0-85071859156 09/10/2019
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(Schultz KM, Fischer MA, Noey EL, Klug CS.) Protein Sci. 2018 Aug;27(8):1407-1417 PMID: 29672978 PMCID: PMC6153404 SCOPUS ID: 2-s2.0-85047394661 04/20/2018
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(Schultz KM, Klug CS.) Appl Magn Reson. 2017 Dec;48(11-12):1341-1353 PMID: 29332998 PMCID: PMC5761346 01/16/2018