
Candice S. Klug, PhD
James S. Hyde Professor of Biophysics; Director, National Biomedical EPR Center; Program Director, Biophysics Graduate Program
Locations
- Biophysics
MFRC 2026
Contact Information
Education
PhD, Biophysics, Medical College of Wisconsin, Milwaukee, WI, 1999
BS, Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 1994
Biography
Methodologies and Techniques
- Adenosine Triphosphatases
- Amino Acid Motifs
- ArnT
- Arrestin
- Arrestins
- ATP-Binding Cassette Transporters
- Bacterial Proteins
- electron paramagnetic resonance spectroscopy
- Electron Spin Resonance Spectroscopy
- Escherichia coli Proteins
- Lipid A
- Lipopolysaccharides
Leadership Positions
- Acting Director, IDP Program in Biomedical Sciences, 2019-2020
- Associate Director, National Biomedical EPR Center, 2014-2016
- Chair, Graduate School Curriculum and Programs Committee, 2010-2013
- Chair, Women in Science Advisory Committee, 2011 - 2016
- Chair, Women's Faculty Council, 2006-2007
- Director, National Biomedical EPR Center, 2016 - present
- President, MCW Faculty Council, 2016-2017
- Program Director, Biophysics Graduate Program, 2013 - present
- Training Program Director, National Biomedical EPR Center, 2003-present
MCW Program / Core Facilities
- Biophysics Graduate Program
- Interdisciplinary Program in the Biomedical Sciences
- National Biomedical EPR Center
Research Interests
Lpt Protein-Mediated Transport of LPS is an R01 award from the National Institute of General Medical Sciences. The goal of this project is to gain insights into the mechanism of LPS transport across the periplasm of Gram-negative bacteria using site-directed spin labeling EPR spectroscopy, complementary biophysical approaches and in vivo growth assays to enable rational antibiotic drug design.
Conformation and Functional Dynamics of a Bacterial PASTA Kinase is a multi-PI (with Dr. Christopher J. Kristich) R01 award from the National Institute of General Medical Sciences. The goal of this grant is to study the conformation and orientation of the intact IreK PASTA module in solution and in full-length IreK embedded in a membrane and elucidate the dynamic changes in the kinase domain associated with kinase activation using a complementary combination of in vivo functional analyses and biophysical approaches.
Development of High-Throughput, High-Sensitivity EPR Sample Handling Capabilities for Biomedical Research is a multi-PI (with Dr. Michael Lerch) R01 award from the National Institute of General Medical Sciences. The goal of this grant is to develop two innovative EPR spectrometer technologies with outstanding sample sensitivity that are easy to use and widely available to the scientific community.
Lab Members
Nicholas Cina, Graduate Student
Karson Hilgendorf, Research Technologist I
Kathryn Schultz, Research Scientist I
Publications
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The Two Non-Visual Arrestins Engage ERK2 Differently.
(Perry-Hauser NA, Hopkins JB, Zhuo Y, Zheng C, Perez I, Schultz KM, Vishnivetskiy SA, Kaya AI, Sharma P, Dalby KN, Chung KY, Klug CS, Gurevich VV, Iverson TM.) J Mol Biol. 2022 Apr 15;434(7):167465 PMID: 35077767 PMCID: PMC8977243 SCOPUS ID: 2-s2.0-85124130291 01/26/2022
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Use of Site-Directed Spin Labeling EPR Spectroscopy to Study Protein-LPS Interactions.
(Schultz KM, Klug CS.) Methods Mol Biol. 2022;2548:83-96 PMID: 36151493 SCOPUS ID: 2-s2.0-85138460647 09/24/2022
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(Chen Q, Zhuo Y, Sharma P, Perez I, Francis DJ, Chakravarthy S, Vishnivetskiy SA, Berndt S, Hanson SM, Zhan X, Brooks EK, Altenbach C, Hubbell WL, Klug CS, Iverson TM, Gurevich VV.) J Mol Biol. 2021 Feb 19;433(4):166790 PMID: 33387531 PMCID: PMC7870585 SCOPUS ID: 2-s2.0-85100159529 01/03/2021
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(Klug, C.S., Lerch, M.T., Feix, J.B. .) Nitroxides: Synthesis, Properties, and Applications. Klug, C.S., Lerch, M.T., Feix, J.B. (2021) Applications of nitroxide spin labels to structural biology in: Nitroxides: Synthesis, Properties, and Applications. (Ouari, O., Gigmes, D., eds.) Royal Society of Chemistry, pp. 392-419. 05/14/2021
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A non-GPCR-binding partner interacts with a novel surface on β-arrestin1 to mediate GPCR signaling.
(Zhuo Y, Gurevich VV, Vishnivetskiy SA, Klug CS, Marchese A.) J Biol Chem. 2020 Oct 09;295(41):14111-14124 PMID: 32753481 PMCID: PMC7549033 SCOPUS ID: 2-s2.0-85092803357 08/06/2020
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Rutile dielectric loop-gap resonator for X-band EPR spectroscopy of small aqueous samples.
(Mett RR, Sidabras JW, Anderson JR, Klug CS, Hyde JS.) J Magn Reson. 2019 Oct;307:106585 PMID: 31499469 PMCID: PMC6948142 SCOPUS ID: 2-s2.0-85071859156 09/10/2019
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(Schultz KM, Fischer MA, Noey EL, Klug CS.) Protein Sci. 2018 Aug;27(8):1407-1417 PMID: 29672978 PMCID: PMC6153404 SCOPUS ID: 2-s2.0-85047394661 04/20/2018
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Characterization of and lipopolysaccharide binding to the E. coli LptC protein dimer.
(Schultz KM, Klug CS.) Protein Sci. 2018 Feb;27(2):381-389 PMID: 29024084 PMCID: PMC5775163 SCOPUS ID: 2-s2.0-85040841909 10/13/2017
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(Schultz KM, Klug CS.) Appl Magn Reson. 2017 Dec;48(11-12):1341-1353 PMID: 29332998 PMCID: PMC5761346 01/16/2018
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Structural basis of arrestin-3 activation and signaling.
(Chen Q, Perry NA, Vishnivetskiy SA, Berndt S, Gilbert NC, Zhuo Y, Singh PK, Tholen J, Ohi MD, Gurevich EV, Brautigam CA, Klug CS, Gurevich VV, Iverson TM.) Nat Commun. 2017 Nov 10;8(1):1427 PMID: 29127291 PMCID: PMC5681653 SCOPUS ID: 2-s2.0-85033606940 11/12/2017
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Overview of arrestin mediated signaling with receptors and non-receptor binding partners
(Zhuo Y, Klug CS.) The Structural Basis of Arrestin Functions. 24 May 2017:19-29 SCOPUS ID: 2-s2.0-85034009393 05/24/2017
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Directed evolution provides insight into conformational substrate sampling by SrtA.
(Suliman M, Santosh V, Seegar TCM, Dalton AC, Schultz KM, Klug CS, Barton WA.) PLoS One. 2017;12(8):e0184271 PMID: 28859178 PMCID: PMC5578623 SCOPUS ID: 2-s2.0-85028825732 09/01/2017