Medical College of Wisconsin
Medical College of Wisconsin Medical College of Wisconsin
Proteins2_Hero
Biophysics
Candice Klug

Candice S. Klug, PhD

James S. Hyde Professor of Biophysics; Director, National Biomedical EPR Center; Program Director, Biophysics Graduate Program

Locations

  • Biophysics
    MFRC 2026

Contact Information

Education

Postdoctoral Fellow in Ophthalmic Biophysical Chemistry, UCLA School of Medicine, Jules Stein Eye Institute, Los Angeles, CA, 1999-2001
PhD, Biophysics, Medical College of Wisconsin, Milwaukee, WI, 1999
BS, Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 1994

Biography

My research interests currently focus on protein structure and dynamics. I use the SDSL (site-directed spin labeling) EPR spectroscopy technique to study the functional dynamics of soluble and membrane proteins essential to bacterial viability.

Methodologies and Techniques

  • Adenosine Triphosphatases
  • Amino Acid Motifs
  • ArnT
  • Arrestin
  • Arrestins
  • ATP-Binding Cassette Transporters
  • Bacterial Proteins
  • electron paramagnetic resonance spectroscopy
  • Electron Spin Resonance Spectroscopy
  • Escherichia coli Proteins
  • Lipid A
  • Lipopolysaccharides

Leadership Positions

  • Acting Director, IDP Program in Biomedical Sciences, 2019-2020
  • Associate Director, National Biomedical EPR Center, 2014-2016
  • Chair, Graduate School Curriculum and Programs Committee, 2010-2013
  • Chair, Women in Science Advisory Committee, 2011 - 2016
  • Chair, Women's Faculty Council, 2006-2007
  • Director, National Biomedical EPR Center, 2016 - present
  • President, MCW Faculty Council, 2016-2017
  • Program Director, Biophysics Graduate Program, 2013 - present
  • Training Program Director, National Biomedical EPR Center, 2003-present

MCW Program / Core Facilities

  • Biophysics Graduate Program
  • Interdisciplinary Program in the Biomedical Sciences
  • National Biomedical EPR Center

Research Interests

Lpt Protein-Mediated Transport of LPS is an R01 award from the National Institute of General Medical Sciences. The goal of this project is to gain insights into the mechanism of LPS transport across the periplasm of Gram-negative bacteria using site-directed spin labeling EPR spectroscopy, complementary biophysical approaches and in vivo growth assays to enable rational antibiotic drug design.

Conformation and Functional Dynamics of a Bacterial PASTA Kinase is a multi-PI (with Dr. Christopher J. Kristich) R01 award from the National Institute of General Medical Sciences. The goal of this grant is to study the conformation and orientation of the intact IreK PASTA module in solution and in full-length IreK embedded in a membrane and elucidate the dynamic changes in the kinase domain associated with kinase activation using a complementary combination of in vivo functional analyses and biophysical approaches.

Development of High-Throughput, High-Sensitivity EPR Sample Handling Capabilities for Biomedical Research is a multi-PI (with Dr. Michael Lerch) R01 award from the National Institute of General Medical Sciences. The goal of this grant is to develop two innovative EPR spectrometer technologies with outstanding sample sensitivity that are easy to use and widely available to the scientific community.

Lab Members
Nicholas Cina, Graduate Student
Karson Hilgendorf, Research Technologist I
Kathryn Schultz, Research Scientist I

Publications

Publications of Candice S. Klug PhD from the Faculty Collaboration Database