PhD, Oregon Health & Sciences University, 1997
Postdoctoral Fellow, Harvard University, 2001
BS, University of California, San Diego, CA, 1991
The overarching research goal of the Link lab is to study the cellular basis of signaling and the role in development and relationships to disease processes. We primarily use zebrafish for our studies, combining imaging based technologies and genome editing. As part of this research we have developed tools to monitor and manipulate basic cellular processes such as endocytosis, vesicle trafficking and nuclear dynamics. In addition, we have created transgenic animals to investigate and quantitate signaling networks including Notch, BMP/Smad, and Hippo-Yap/Taz. There are several areas of research we are currently focused. One goal is to understand the cell biology and signaling events critical for ocular development and disease. Currently we are studying how endocytosis and polarized vesicle trafficking affect signaling, primarily Notch, Wnt and Hippo-Yap/Taz pathways, in neuroepithelia and how these pathways interact to regulate neurogenesis. Ancillary to these studies, we are characterizing the role of Yap/Taz activity in retinal pigment epithelial development and maintenance. For ocular disease studies, we are characterizing the cellular mechanisms underlying pathology in several eye disorders including PhR degenerations, glaucomas, and myopia. Integrated with all these research goals, the Link lab emphasizes collaborative research and career training for students and post-docs.
Movie 1. In vivo confocal time-lapse movie shows heterogeneity of interkinetic nuclear migration in retinal progenitors from 24 hpf.
Images are composed of compressed z-stacks of H2A-GFP fluorescence overlaid with single mid-plane bright-field images. Apical and basal surfaces are outlined with dashed white lines in the first and last frame and an arrow indicates a cell undergoing mitosis at the apical surface. The movie plays at 6 frames per second covering 12 hr of development time (Baye and Link, 2007).
Specifically, neuroepithelia with deep basal nuclear migrations and shorter cell cycles are biased to produce neurons, rather than give rise to daughter cells that re-enter the cell cycle. The lab is currently exploring the signals and mechanisms underlying these relationships.
Complex gene interactions underlying glaucoma-phenotypes
The overall goal of these studies is to understand the relationships between genes that contribute to glaucoma-phenotypes. The glaucomas are a heterogeneous group of ocular disorders characterized by retinal ganglion cell death, optic nerve damage and visual field loss. Elevated intraocular pressure is a principal risk factor for this neurodegenerative disease. The majority and perhaps all forms of glaucoma are complex – requiring the interaction of multiple genes. While several genes that contribute to glaucoma have been identified, many additional loci remain unknown. We are using zebrafish to identify and study genes which promote glaucoma-phenotypes (Figure 3).
In particular, we are focusing on the complex-gene interactions that promote retinal ganglion cell death in the context of elevated intraocular pressure. Additional projects are focused on genes that regulate the development of glaucoma-relevant ocular tissues, as such genes have previously been shown to impact glaucoma (Movie 2).
Movie 2. In vivo confocal time-lapse movie showing migration of neural crest cells into the periocular region of the eye. These cells, along with head mesoderm, contribute to the structures of the anterior segment that regulate intraocular pressure. Cells were labeled with the foxd3:GFP transgene (Gilmour et al. Neuron 34:577-88, 2002)
(Wilk MA, Huckenpahler AL, Collery RF, Link BA, Carroll J.) Transl Vis Sci Technol. 2017 Apr;6(2):8.
(Lewis TR, Kundinger SR, Pavlovich AL, Bostrom JR, Link BA, Besharse JC.) Dev Biol. 2017 05 15;425(2):176-190.
(Henle SJ, Link BA.) Stem Cell Investig. 2017;4:16.
(Huckenpahler AL, Wilk MA, Cooper RF, Moehring F, Link BA, Carroll J, Collery RF.) Vis Neurosci. 2016 01;33:E011.
(Collery RF, Volberding PJ, Bostrom JR, Link BA, Besharse JC.) Invest Ophthalmol Vis Sci. 2016 12 01;57(15):6805-6814.
(Hilinski WC, Bostrom JR, England SJ, Juárez-Morales JL, de Jager S, Armant O, Legradi J, Strähle U, Link BA, Lewis KE.) Neural Dev. 2016 08 23;11(1):16.
(Link BA, Collery RF.) Annu Rev Vis Sci. 2015 Nov 24;1:125-153.
(Miesfeld JB, Gestri G, Clark BS, Flinn MA, Poole RJ, Bader JR, Besharse JC, Wilson SW, Link BA.) Development. 2015 Sep 01;142(17):3021-32.
(Porazinski S, Wang H, Asaoka Y, Behrndt M, Miyamoto T, Morita H, Hata S, Sasaki T, Krens SFG, Osada Y, Asaka S, Momoi A, Linton S, Miesfeld JB, Link BA, Senga T, Shimizu N, Nagase H, Matsuura S, Bagby S, Kondoh H, Nishina H, Heisenberg CP, Furutani-Seiki M.) Nature. 2015 May 14;521(7551):217-221.
(Collery RF, Veth KN, Dubis AM, Carroll J, Link BA.) PLoS One. 2014;9(10):e110699.
(Paulus JD, Link BA.) PLoS One. 2014;9(10):e109922.
(Yeo NC, O'Meara CC, Bonomo JA, Veth KN, Tomar R, Flister MJ, Drummond IA, Bowden DW, Freedman BI, Lazar J, Link BA, Jacob HJ.) Genome Res. 2015 Jan;25(1):57-65.