American Board of Pediatrics - 1981
American Board of Medical Genetics
Clinical Genetics - 1982
Clinical Biochemical Genetics – 1993, 2003, 2005, 2007, 2009
Laboratory Name: Fatty Acid Oxidation
LAP Number: 1780401
CAP Accreditation Expires: June 2016
CLIA Identification Number: 52D0661957, expires 01/2017
Medicare Number: 160058
Medi-Cal Provider Number: XYP173950
State License Number: Wisconsin 42007
Federal Tax ID Number: 39-0806261N
HCFA License Number: 16-0058
UPIN #: A01404
Please download the Fatty Acid Oxidation Disorders Screening Tests Requisition Form (PDF) and include in the shipment of the skin fibroblasts.
Patient and Billing Information
For cultures sent by mail to our laboratory, we only bill the referring hospital or laboratory for these tests. We suggest having the Department of Pathology in your hospital send the sample to us. The requisition form should be included with fibroblast cultures and clinical and biochemical information.
Ship two T-25 (25cm2) flasks of confluent fibroblasts obtained from skin biopsy and routine fibroblast tissue culture to the above address by overnight express accompanied by clinical and biochemical data and the billing information sheet.
- 3H-Acyl-carnitine profiling by radio-HPLC in intact cultured fibroblast monolayers to screen for all known disorders of β-oxidation, excluding riboflavin-responsive variants and all methylmalonic acidemias: $1174.25 (CPT codes 88233 + 88233 + 82493), including physician interpretation (CPT code 80500).
- Immunoblotting for short-, medium-, long-, and very-long chain acyl-CoA dehydrogenase and α- and β- electron transfer flavoprotein subunits in fractionated cultured fibroblasts: $922.00 for any of the above polypeptides (CPT codes 88372), including physician interpretation (CPT code 80500).
- Immunoblotting for α- and β- electron transfer flavoprotein subunits in fractionated cultured fibroblasts: $1720.00 for any of the above polypeptides (CPT code 88372 x 2, two bands).
Prenatal diagnosis on amniocytes is possible for virtually all fatty acid oxidation disorders. However, before we will accept amniocyte samples from a referring laboratory, please contact William J. Rhead, MD, PhD, directly at (414) 266-2906. In addition to 2 confluent T-25 flasks of amniocytes from the proband fetus, we require amniocytes from 2 anonymous control pregnancies, 2 T-25s each. Further details can be obtained directly from Dr. Rhead.
3H-Acyle-carnitine profiling by radio-HPLC in amniocytes to screen for all known disorders of β-oxidation, excluding riboflavin-responsive varians and all methylamlonic acidemias: $1174.25 (CPT codes 88235+82492), with physician interpretation (CPT code 80500).
These analyses are non-experimental, non-investigational and not in conflict with medical standards. This laboratory and Children's Hospital of Wisconsin has been accredited by the Joint Commission on Accreditation of Healthcare Organizations, the College of American Pathologists, CLIA (16D0664504), and HCFA (license number 16-0058). Dr. Rhead is board-certified in Clinical Biochemical Genetics by the American Board of Medical Genetics.
Explanation of CPT Codes
88233 Skin fibroblast culture
82492 Chromatography, quantitative, column (eg, gas liquid or HPLC): multiple analytes, sincle stationary and mobile phase
88235 Tissue culture for non-neoplastic disorders: amniotic fluid or chrorionic villus cells
88372 Protein a nalysis of tissue by Western Blot, with interpretation and report, one band
83918 Physician interpretation