Our faculty hold these EPR-related grants.
PI: Candice Klug*
National Biomedical EPR Center
The mission of the EPR Center is to serve the community of EPR spectroscopists by development of advanced EPR instrumentation and new EPR methodology.
The broad aim of the National Biomedical EPR Center is to create and maintain a comprehensive center with balance in all five categories of a Research Resource:
- Technological research:
(1) Resonator development
(2) W-band enhancement
(3) Broadband digital detection, signal analysis, and archiving in EPR spectroscopy
(4) Microwave oscillator development
(5) H1||H0 (parallel mode) EPR at Q- and W-band
(6) X-band/Q-band spin trapping methodology development: Structure/function aspects of NOS-generated radicals
- Dissemination, including one workshop during the funded period
(1) Graduate and post-graduate training
(2) Training established investigators
(3) Training of young investigators
The functions of a Research Resource will be accomplished with expertise in the three main application areas of EPR spectroscopy: free radicals, transition metals, and spin labels and with competence in EPR instrumental development.
*Named PI & director on 01/06/16 upon retirement of James Hyde, original PI
LptA-Mediated Transport of LPS
The proposed studies focus on how the periplasmic protein LptA receives LPS from the IM-associated protein LptC, how LptA protects the hydrophobic acyl chains of LPS as it crosses the periplasm, and how LptA delivers LPS to LptDE at the OM. Genetic screenings, laser light scattering analyses, EPR spectroscopy studies, and isothermal titration calorimetry (ITC) measurements will provide detailed insights into the mechanism of LPS transport across the periplasm of Gram-negative bacteria.
Cholesterol Crystalline Domain Function in Eye Lens: EPR Spin-Labeling Studies
The long-term objective of this proposal is to achieve a greater understanding of the function of cholesterol in fiber cell membranes.
Mechanism of Activation and Membrane Interactions of Pseudomonas Toxin ExoU
In this project biochemical and biophysical studies will be used to elucidate the molecular mechanism of activation for the phospholipase ExoU, with a long term goal of facilitating the development of novel inhibitors to reduce tissue damage or sepsis due to P. aeruginosa infection.