MCW Cancer Center Redox and Bioenergetics Shared Resource Publications

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  2017 Publications

Zielonka J, Hardy M, Michalski R, Sikora A, Zielonka M, Cheng G, Ouari O, Podsiadły R, Kalyanaraman B. Recent Developments in the Probes and Assays for Measurement of the Activity of NADPH Oxidases. Cell Biochem Biophys. 2017 Jun 29. doi: 10.1007/s12013-017-0813-6. [Epub ahead of print] PubMed PMID: 28660426

Zielonka J, Joseph J, Sikora A, Hardy M, Ouari O, Vasquez-Vivar J, Cheng G, Lopez M, Kalyanaraman B. Mitochondria-Targeted Triphenylphosphonium-Based Compounds: Syntheses, Mechanisms of Action, and Therapeutic and Diagnostic Applications. Chem Rev. 2017 Jun 27. doi: 10.1021/acs.chemrev.7b00042. [Epub ahead of print] PubMed PMID: 28654243

Starenki D, Hong SK, Wu PK, Park JI. Vandetanib and cabozantinib potentiate mitochondria-targeted agents to suppress medullary thyroid carcinoma cells. Cancer Biol Ther. 2017 May 5:1-11. doi: 10.1080/15384047.2017.1323594. [Epub ahead of print] PubMed PMID: 28475408

Egea J, Fabregat I, Frapart YM, Ghezzi P, Görlach A, Kietzmann T, Kubaichuk K, Knaus UG, Lopez MG, Olaso-Gonzalez G, Petry A, Schulz R, Vina J, Winyard P, Abbas K, Ademowo OS, Afonso CB, Andreadou I, Antelmann H, Antunes F, Aslan M, Bachschmid MM, Barbosa RM, Belousov V, Berndt C, Bernlohr D, Bertrán E, Bindoli A, Bottari SP, Brito PM, Carrara G, Casas AI, Chatzi A, Chondrogianni N, Conrad M, Cooke MS, Costa JG, Cuadrado A, My-Chan Dang P, De Smet B, Debelec-Butuner B, Dias IHK, Dunn JD, Edson AJ, El Assar M, El-Benna J, Ferdinandy P, Fernandes AS, Fladmark KE, Förstermann U, Giniatullin R, Giricz Z, Görbe A, Griffiths H, Hampl V, Hanf A, Herget J, Hernansanz-Agustín P, Hillion M, Huang J, Ilikay S, Jansen-Dürr P, Jaquet V, Joles JA, Kalyanaraman B, Kaminskyy D, Karbaschi M, Kleanthous M, Klotz LO, Korac B, Korkmaz KS, Koziel R, Kračun D, Krause KH, Křen V, Krieg T, Laranjinha J, Lazou A, Li H, Martínez-Ruiz A, Matsui R, McBean GJ, Meredith SP, Messens J, Miguel V, Mikhed Y, Milisav I, Milković L, Miranda-Vizuete A, Mojović M, Monsalve M, Mouthuy PA, Mulvey J, Münzel T, Muzykantov V, Nguyen ITN, Oelze M, Oliveira NG, Palmeira CM, Papaevgeniou N, Pavićević A, Pedre B, Peyrot F, Phylactides M, Pircalabioru GG, Pitt AR, Poulsen HE, Prieto I, Rigobello MP, Robledinos-Antón N, Rodríguez-Mañas L, Rolo AP, Rousset F, Ruskovska T, Saraiva N, Sasson S, Schröder K, Semen K, Seredenina T, Shakirzyanova A, Smith GL, Soldati T, Sousa BC, Spickett CM, Stancic A, Stasia MJ, Steinbrenner H, Stepanić V, Steven S, Tokatlidis K, Tuncay E, Turan B, Ursini F, Vacek J, Vajnerova O, Valentová K, Van Breusegem F, Varisli L, Veal EA, Yalçın AS, Yelisyeyeva O, Žarković N, Zatloukalová M, Zielonka J, Touyz RM, Papapetropoulos A, Grune T, Lamas S, Schmidt HHHW, Di Lisa F, Daiber A. European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS). Redox Biol. 2017 May 18;13:94-162. doi: 10.1016/j.redox.2017.05.007. [Epub ahead of print] Review. PubMed PMID: 28577489; PubMed Central PMCID: PMC5458069

Kalyanaraman B, Cheng G, Hardy M, Ouari O, Sikora A, Zielonka J, Dwinell MB. Modified Metformin as a More Potent Anticancer Drug: Mitochondrial Inhibition, Redox Signaling, Antiproliferative Effects and Future EPR Studies. Cell Biochem Biophys. 2017 Apr 21. doi: 10.1007/s12013-017-0796-3. [Epub ahead of print] PubMed PMID: 28429253

Kalyanaraman B, Cheng G, Hardy M, Ouari O, Sikora A, Zielonka J, Dwinell M. Mitochondria-targeted metformins: anti-tumour and redox signalling mechanisms. Interface Focus. 2017 Apr 6;7(2):20160109. doi: 10.1098/rsfs.2016.0109. Review. PubMed PMID: 28382202; PubMed Central PMCID: PMC5311906.

Langley M, Ghosh A, Charli A, Sarkar S, Ay M, Luo J, Zielonka J, Brenza T, Bennett B, Jin H, Ghaisas S, Schlichtmann B, Kim D, Anantharam V, Kanthasamy A, Narasimhan B, Kalyanaraman B, Kanthasamy AG. Mito-Apocynin Prevents Mitochondrial Dysfunction, Microglial Activation, Oxidative Damage, and Progressive Neurodegeneration in MitoPark Transgenic Mice. Antioxid Redox Signal. 2017 Apr 4. doi: 10.1089/ars.2016.6905. [Epub ahead of print] PubMed PMID: 28375739

  2016 Publications

Cheng G, Zielonka J, Ouari O, Lopez M, McAllister D, Boyle K, Barrios CS, Weber JJ, Johnson BD, Hardy M, et al. Mitochondria-Targeted Analogues of Metformin Exhibit Enhanced Antiproliferative and Radiosensitizing Effects in Pancreatic Cancer Cells. Cancer Res. 2016 Jul 1;76(13):3904-15.

Zielonka J, Zielonka M, VerPlank L, Cheng G, Hardy M, Ouari O, Ayhan MM, Podsiadly R, Sikora A, Lambeth JD, Kalyanaraman B. Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit Peroxynitrite Formation. J Biol Chem. 2016; 291:7029-44.

Debski D, Smulik R, Zielonka J, Michalowskie B, Jakubowska M, Debowska K, Adamus J, Marcinek A, Kalyanaraman B, Kisora A. Mechanism of oxidative conversion of Amplex® Red to resorufin: pulse radiolysis and enzymatic studies. Free Radic Biol Med. 2016; 95:323-32.

Kalyanaraman B, Hardy M, Zielonka J. A Critical Review of Methodologies to Detect Reactive Oxygen and Nitrogen Species Stimulated by NADPH Oxidase Enzymes: Implications in Pesticide Toxicity. Curr Pharmacol Rep. 2016; 2:193.

Zielonka J, Podsiadly R, Zielonka M, Hardy M, Kalyanaraman B. On the use of peroxy-caged luciferin (PCL-1) probe for bioluminescent detection of inflammatory oxidants in vitro and in vivo - Identification of reaction intermediates and oxidant-specific minor products. Free Radic Biol Med. 2016; 99:32-42.

Kalyanaraman B, Hardy M, Podsiadly R, Cheng G, Zielonka J. Recent developments in detection of superoxide radical anion and hydrogen peroxide: Opportunities, challenges, and implications in redox signaling. Arch Biochem Biophys. 2016; In press.

Kutty RG, Gang X, Schauder DM, Cossette SM, Bordas M, Cui W, Ramchandran R. Dual Specificity Phosphatase 5 Is Essential for T Cell Survival. PLOS ONE 11, no. 12 (2016): e0167246.

  2015 Publications

Zielonka, J., Sikora, A., Adamus, J. and Kalyanaraman, B., 2015. Detection and differentiation between peroxynitrite and hydroperoxides using mitochondria-targeted arylboronic acid. Mitochondrial Medicine: Volume I, Probing Mitochondrial Function, pp.171-181.

Cheng G, Zielonka J, McAllister D, Hardy M, Ouari O, Joseph J, Dwinell MB, Kalyanaraman B. Antiproliferative effects of mitochondria-targeted cationic antioxidants and analogs: Role of mitochondrial bioenergetics and energy-sensing mechanism. Cancer Lett 365:96-106, 2015.

Kittipongdaja W, Wu X, Garner J, Liu X, Komas SM, Hwang ST, Schieke SM. Rapamycin Suppresses Tumor Growth and Alters the Metabolic Phenotype in T-Cell Lymphoma. J Invest Dermatol 135:2301–8, 2015.

Zhang Q, Pan J, Lubet RA, Komas SM, Kalyanaraman B, Wang Y, You M. Enhanced Antitumor activity of 3-bromopyruvate in combination with rapamycin in vivo and in vitro. Cancer Prev Res (Phila) 8:318–26, 2015.

Roy I, McAllister DM, Gorse E, Dixon K, Piper CT, Zimmerman NP, Getschman AE, Tsai S, Engle DD, Evans D, Volkman BF, Kalyanaraman B, Dwinell MB. Chemokine biased agonism regulates pancreatic cancer migration and metastasis through bioenergetics signaling. Cancer Res (in press).

  2014 Publications

Smulik, R., Dębski, D., Zielonka, J., Michałowski, B., Adamus, J., Marcinek, A., Kalyanaraman, B. and Sikora, A., 2014. Nitroxyl (HNO) reacts with molecular oxygen and forms peroxynitrite at physiological pH biological implications. Journal of Biological Chemistry, 289(51), pp.35570-35581.

Shah, G., Zielonka, J., Chen, F., Zhang, G., Cao, Y., Kalyanaraman, B. and See, W., 2014. H 2 O 2 Generation by bacillus Calmette-Guérin Induces the Cellular Oxidative Stress Response Required for bacillus Calmette-Guérin Direct Effects on Urothelial Carcinoma Biology. The Journal of urology, 192(4), pp.1238-1248.

Michalski, R., Zielonka, J., Gapys, E., Marcinek, A., Joseph, J. and Kalyanaraman, B., 2014. Real-time measurements of amino acid and protein hydroperoxides using coumarin boronic acid. Journal of Biological Chemistry, 289(32), pp.22536-22553.

Hardy, M., Poulhés, F., Rizzato, E., Rockenbauer, A., Banaszak, K., Karoui, H., Lopez, M., Zielonka, J., Vasquez-Vivar, J., Sethumadhavan, S. and Kalyanaraman, B., 2014. Mitochondria-targeted spin traps: synthesis, superoxide spin trapping, and mitochondrial uptake. Chemical research in toxicology, 27(7), pp.1155-1165.

Koto, T., Michalski, R., Zielonka, J., Joseph, J. and Kalyanaraman, B., 2014. Detection and identification of oxidants formed during• NO/O2•–reaction: A multi-well plate CW-EPR spectroscopy combined with HPLC analyses. Free radical research, 48(4), pp.478-486.

Kalyanaraman, B., Dranka, B.P., Hardy, M., Michalski, R. and Zielonka, J., 2014. HPLC-based monitoring of products formed from hydroethidine-based fluorogenic probes—the ultimate approach for intra-and extracellular superoxide detection. Biochimica et Biophysica Acta (BBA)-General Subjects, 1840(2), pp.739-744.

Michalski, R., Michalowski, B., Sikora, A., Zielonka, J. and Kalyanaraman, B., 2014. On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: a reassessment. Free Radical Biology and Medicine, 67, pp.278-284.

Pan J., Zhang Q., Liu Q., Komas S.M., Kalyanaraman B., Lubet R.A., Wang Y., You M. Honokiol inhibits lung tumorigenesis through inhibition of mitochondrial function. Cancer Prev Res (Phila) 7:1149–59, 2014.

Cheng G., Zielonka J., McAllister D., Tsai S., Dwinell M.B., Kalyanaraman B. Profiling and targeting of cellular bioenergetics: inhibition of pancreatic cancer cell proliferation. Br J Cancer 111:85–93, 2014.

Mouradian M., Kikawa K.D., Dranka B.P., Komas S.M., Kalyanaraman B. and Pardini R.S. (2014). Docosahexaenoic acid attenuates breast cancer cell metabolism and the Warburg phenotype by targeting bioenergetic function. Mol. Carcinog.. doi: 10.1002/mc.2215

Zielonka J., Cheng G., Zielonka M., Ganesh T., Sun A., Joseph J., Michalski R., O'Brien W.J., Lambeth J.D., Kalyanaraman B. (2014). High-throughput Assays for Superoxide and Hydrogen Peroxide: Design of a Screening Workflow to Identify Inhibitors of NADPH Oxidases. J Biol Chem.

Matthew D. Greseth and Paula Traktman (2014). De novo Fatty Acid Biosynthesis Contributes Significantly to Establishment of a Bioenergetically Favorable Environment for Vaccinia Virus Infection. PLoS Pathog.

Cheng G., Zielonka J., McAllister D., Tsai S., Dwinell M.B., and Kalyanaraman B. (2014). Profiling and targeting of cellular bioenergetics: inhibition of pancreatic cancer cell proliferation. British Journal of Cancer (2014) 111, 85–93.

  2013 Publications

Zielonka, J., Lambeth, J.D. and Kalyanaraman, B., 2013. On the use of L-012, a luminol-based chemiluminescent probe, for detecting superoxide and identifying inhibitors of NADPH oxidase: a reevaluation. Free Radical Biology and Medicine, 65, pp.1310-1314.

Dickey, J.S., Gonzalez, Y., Aryal, B., Mog, S., Nakamura, A.J., Redon, C.E., Baxa, U., Rosen, E., Cheng, G., Zielonka, J. and Parekh, P., 2013. Mito-tempol and dexrazoxane exhibit cardioprotective and chemotherapeutic effects through specific protein oxidation and autophagy in a syngeneic breast tumor preclinical model. PLoS One, 8(8), p.e70575.

Sikora, A., Zielonka, J., Adamus, J., Debski, D., Dybala-Defratyka, A., Michalowski, B., Joseph, J., Hartley, R.C., Murphy, M.P. and Kalyanaraman, B., 2013. Reaction between peroxynitrite and triphenylphosphonium-substituted arylboronic acid isomers: identification of diagnostic marker products and biological implications. Chemical research in toxicology, 26(6), pp.856-867.

Michalski, R., Zielonka, J., Hardy, M., Joseph, J. and Kalyanaraman, B., 2013. Hydropropidine: a novel, cell-impermeant fluorogenic probe for detecting extracellular superoxide. Free Radical Biology and Medicine, 54, pp.135-147.

Zielonka, J., Joseph, J., Sikora, A. and Kalyanaraman, B., 2013. Real-time monitoring of reactive oxygen and nitrogen species in a multiwell plate using the diagnostic marker products of specific probes. Methods Enzymol, 526, pp.145-157.

Starenki, D. and Park, J.I., 2013. Mitochondria-targeted nitroxide, Mito-CP, suppresses medullary thyroid carcinoma cell survival in vitro and in vivo. The Journal of Clinical Endocrinology & Metabolism, 98(4), pp.1529-1540.

Cheng, G., Zielonka, J., McAllister, D.M., Mackinnon, A.C., Joseph, J., Dwinell, M.B. and Kalyanaraman, B., 2013. Mitochondria-targeted vitamin E analogs inhibit breast cancer cell energy metabolism and promote cell death. BMC cancer, 13(1), p.285.

Dranka, B.P., Gifford, A., Ghosh, A., Zielonka, J., Joseph, J., Kanthasamy, A.G. and Kalyanaraman, B., 2013. Diapocynin prevents early Parkinson's disease symptoms in the leucine-rich repeat kinase 2 (LRRK2 R1441G) transgenic mouse. Neuroscience letters, 549, pp.57-62.

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  2012 Publications

Zielonka, J., Sikora, A., Hardy, M., Joseph, J., Dranka, B.P. and Kalyanaraman, B., 2012. Boronate probes as diagnostic tools for real time monitoring of peroxynitrite and hydroperoxides. Chemical research in toxicology, 25(9), pp.1793-1799.

Zielonka, J., Zielonka, M., Sikora, A., Adamus, J., Joseph, J., Hardy, M., Ouari, O., Dranka, B.P. and Kalyanaraman, B., 2012. Global profiling of reactive oxygen and nitrogen species in biological systems high-throughput real-time analyses. Journal of Biological Chemistry, 287(5), pp.2984-2995.

Mukhopadhyay, P., Horváth, B., Zsengellér, Z., Zielonka, J., Tanchian, G., Holovac, E., Kechrid, M., Patel, V., Stillman, I.E., Parikh, S.M. and Joseph, J., 2012. Mitochondrial-targeted antioxidants represent a promising approach for prevention of cisplatin-induced nephropathy. Free Radical Biology and Medicine, 52(2), pp.497-506.

Dranka, B.P., Zielonka, J., Kanthasamy, A.G. and Kalyanaraman, B., 2012. Alterations in bioenergetic function induced by Parkinson’s disease mimetic compounds: lack of correlation with superoxide generation. Journal of neurochemistry, 122(5), pp.941-951.

Cheng, G., Zielonka, J., Dranka, B.P., McAllister, D., Mackinnon, A.C., Joseph, J. and Kalyanaraman, B., 2012. Mitochondria-targeted drugs synergize with 2-deoxyglucose to trigger breast cancer cell death. Cancer research, 72(10), pp.2634-2644.

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