Student Faculty Collaborate

RBSR Publications

These publications contain data gathered or analyses performed utilizing the RBSR facility.


Kalyanaraman B. NAC, NAC, Knockin' on Heaven's door: Interpreting the mechanism of action of N-acetylcysteine in tumor and immune cells. Redox Biol. 2022 Oct 9;57:102497. doi: 10.1016/j.redox.2022.102497. Epub ahead of print. PMID: 36242913; PMCID: PMC9563555.

Patel JJ, Willoughby R, Peterson J, Carver T, Zelten J, Markiewicz A, Spiegelhoff K, Hipp LA, Canales B, Szabo A, Heyland DK, Stoppe C, Zielonka J, Freed JK. High-Dose Intravenous Hydroxocobalamin (Vitamin B12) in Septic Shock: A Double-Blind, Allocation-Concealed, Placebo-Controlled Single-Center Pilot Randomized Controlled Trial (The IV-HOCSS Trial). Chest. 2022 Sep 26:S0012-3692(22)03890-9. doi: 10.1016/j.chest.2022.09.021. PMID: 36174744.

Kim J, Yang Y, Hong SK, Zielonka J, Dash RK, Audi SH, Kumar SN, Joshi A, Zimmerman MA, Hong JC. Fluorescein clearance kinetics in blood and bile indicates hepatic ischemia-reperfusion injury in rats. Am J Physiol Gastrointest Liver Physiol. 2022 Aug 1;323(2):G126-G133. doi: 10.1152/ajpgi.00038.2022. PMID: 35700191.

Michalski R, Smulik-Izydorczyk R, Pięta J, Rola M, Artelska A, Pierzchała K, Zielonka J, Kalyanaraman B, Sikora AB. The Chemistry of HNO: Mechanisms and Reaction Kinetics. Front Chem. 2022 Jul 5;10:930657. doi: 10.3389/fchem.2022.930657. PMID: 35864868; PMCID: PMC9294461.

Pierzchała K, Pięta M, Rola M, Świerczyńska M, Artelska A, Dębowska K, Podsiadły R, Pięta J, Zielonka J, Sikora A, Marcinek A, Michalski R. Fluorescent probes for monitoring myeloperoxidase-derived hypochlorous acid: a comparative study. Sci Rep. 2022 Jun 3;12(1):9314. doi: 10.1038/s41598-022-13317-8. PMID: 35660769; PMCID: PMC9166712.

Vujacic-Mirski K, Oelze M, Kuntic I, Kuntic M, Kalinovic S, Li H, Zielonka J, Münzel T, Daiber A. Measurement of Tetrahydrobiopterin in Animal Tissue Samples by HPLC with Electrochemical Detection-Protocol Optimization and Pitfalls. Antioxidants (Basel). 2022 Jun 16;11(6):1182. doi: 10.3390/antiox11061182. PMID: 35740082; PMCID: PMC9228106.

Kalyanaraman B, Cheng G, Hardy M. Therapeutic Targeting of Tumor Cells and Tumor Immune Microenvironment Vulnerabilities. Front Oncol. 2022 Jun 8;12:816504. doi: 10.3389/fonc.2022.816504. PMID: 35756631; PMCID: PMC9214210.

Murphy MP, Bayir H, Belousov V, Chang CJ, Davies KJA, Davies MJ, Dick TP, Finkel T, Forman HJ, Janssen-Heininger Y, Gems D, Kagan VE, Kalyanaraman B, Larsson NG, Milne GL, Nyström T, Poulsen HE, Radi R, Van Remmen H, Schumacker PT, Thornalley PJ, Toyokuni S, Winterbourn CC, Yin H, Halliwell B. Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo. Nat Metab. 2022 Jun;4(6):651-662. doi: 10.1038/s42255-022-00591-z. Epub 2022 Jun 27. PMID: 35760871; PMCID: PMC9711940.

Pierzchała K, Pięta M, Rola M, Świerczyńska M, Artelska A, Dębowska K, Podsiadły R, Pięta J, Zielonka J, Sikora A, Marcinek A, Michalski R. Fluorescent probes for monitoring myeloperoxidase-derived hypochlorous acid: a comparative study. Sci Rep. 2022 Jun 3;12(1):9314. doi: 10.1038/s41598-022-13317-8. PMID: 35660769; PMCID: PMC9166712.

Vujacic-Mirski K, Oelze M, Kuntic I, Kuntic M, Kalinovic S, Li H, Zielonka J, Münzel T, Daiber A. Measurement of Tetrahydrobiopterin in Animal Tissue Samples by HPLC with Electrochemical Detection-Protocol Optimization and Pitfalls. Antioxidants (Basel). 2022 Jun 16;11(6):1182. doi: 10.3390/antiox11061182. PMID: 35740082; PMCID: PMC9228106.

Zhang Q, Xiong D, Pan J, Wang Y, Hardy M, Kalyanaraman B, You M. Chemoprevention of Lung Cancer with a Combination of Mitochondria-Targeted Compounds. Cancers (Basel). 2022 May 21;14(10):2538. doi: 10.3390/cancers14102538. PMID: 35626143; PMCID: PMC9140024.

Kalyanaraman B. Exploiting the tumor immune microenvironment and immunometabolism using mitochondria-targeted drugs: Challenges and opportunities in racial disparity and cancer outcome research. FASEB J. 2022 Apr;36(4):e22226. doi: 10.1096/fj.202101862R. PMCID: PMC9242412

Huang M, Xiong D, Pan J, Zhang Q, Wang Y, Myers CR, Johnson BD, Hardy M, Kalyanaraman B, You M. Prevention of Tumor Growth and Dissemination by In Situ Vaccination with Mitochondria-Targeted Atovaquone. Adv Sci (Weinh). 2022 Apr;9(12):e2101267. doi: 10.1002/advs.202101267. PMID: 35243806; PMCID: PMC9036031.

Cheng G, Hardy M, You M, Kalyanaraman B. Combining PEGylated Mito-Atovaquone with MCT and Krebs Cycle Redox Inhibitors as a Potential Strategy to Abrogate Tumor Cell Proliferation. Sci Rep. 2022 Mar 24;12(1):5143. doi: 10.1038/s41598-022-08984-6. PMID: 35332210; PMCID: PMC8948292.

Grzelakowska A, Modrzejewska J, Kolińska J, Szala M, Zielonka M, Dębowska K, Zakłos-Szyda M, Sikora A, Zielonka J, Podsiadły R. Water-soluble cationic boronate probe based on coumarin imidazolium scaffold: Synthesis, characterization, and application to cellular peroxynitrite detection. Free Radic Biol Med. 2022 Feb 1;179:34-46. doi: 10.1016/j.freeradbiomed.2021.12.260. Epub 2021 Dec 17. PMID: 34923103.


Yeo CT, Stancill JS, Oleson BJ, Schnuck JK, Stafford JD, Naatz A, Hansen PA, Corbett JA. Regulation of ATR-Dependent DNA Damage Response by Nitric Oxide. J Biol Chem. Jan-Jun 2021; 296:100388. PMID: 33567339; PMCID: PMC7967039

Xin G, Chen Y, Topchyan P, Kasmani MY, Burns R, Volberding PJ, Wu X, Cohn A, Chen Y, Lin CW, Ho PC, Silverstein R, Dwinell MB, Cui W. Targeting PIM1-Mediated Metabolism in Myeloid Suppressor Cells to Treat Cancer. Cancer Immunol Res. 2021 Apr;9(4):454-469. PMID: 33579728; PMCID: PMC8137571

Medhora M, Phadnis P, Narayanan J, Gasperetti T, Zielonka J, Moulder JE, Fish BL, Szabo A. Radiation Increases Bioavailability of Lisinopril, a Mitigator of Radiation-Induced Toxicities. Front Pharmacol. 2021 Apr 27; 12:646076. PMID: 33986677; PMCID: PMC8111401 

Zielonka J, Sikora A, Podsiadly R, Hardy M, Kalyanaraman B. Identification of Peroxynitrite by Profiling Oxidation and Nitration Products from Mitochondria-Targeted Arylboronic Acid. Methods Mol Biol. 2021; 2275:315-327. PMID: 33823245

Kalinovic S, Stamm P, Oelze M, Steven S, Kröller-Schön S, Kvandova M, Zielonka J, Münzel T, Daiber A. Detection of Extracellular Superoxide in Isolated Human Immune Cells and in an Animal Model of Arterial Hypertension Using Hydropropidine Probe and HPLC Analysis. Free Radic Biol Med. 2021 May 20; 168:214-225.

AbuEid M, McAllister DM, McOlash L, Harwig MC, Cheng G, Drouillard D, Boyle KA, Hardy M, Zielonka J, Johnson BD, Hill RB, Kalyanaraman B, Dwinell MB. Synchronous Effects of Targeted Mitochondrial Complex I Inhibitors on Tumor and Immune Cells Abrogate Melanoma Progression. iScience. 2021 May 25; 24(6):102653. PMID: 34189432; PMCID: PMC8220235

Cheng G, Hardy M, Topchyan P, Zander R, Volberding P, Cui W, Kalyanaraman B. Mitochondria-Targeted Hydroxyurea Inhibits OXPHOS and Induces Antiproliferative and Immunomodulatory Effects. iScience. 2021 May 31;24(6):102673. PMID: 34189437; PMCID: PMC8215227

Grzelakowska A, Zielonka M, Dębowska K, Modrzejewska J, Szala M, Sikora A, Zielonka J, Podsiadły R. Two-Photon Fluorescent Probe for Cellular Peroxynitrite: Fluorescence Detection, Imaging, and Identification of Peroxynitrite-Specific Products. Free Radic Biol Med. 2021 Jun; 169:24-35. PMID: 33862158

Kalyanaraman B. Reactive Oxygen Species, Proinflammatory and Immunosuppressive Mediators Induced in COVID-19: Overlapping Biology with Cancer. RSC Chem Biol. 2021 Jun 30; 2(5):1402-1414. PMID: 34704045; PMCID: PMC8496060

Schlichtmann BW, Kalyanaraman B, Schlichtmann RL, Panthani MG, Anantharam V, Kanthasamy AG, Mallapragada SK, Narasimhan B. Functionalized Polyanhydride Nanoparticles for Improved Treatment of Mitochondrial Dysfunction. J Biomed Mater Res B Appl Biomater. 2021 Jul 27. PMID: 34312984

Siarkiewicz P, Michalski R, Sikora A, Smulik-Izydorczyk R, Szala M, Grzelakowska A, Modrzejewska J, Bailey A, Nycz JE, Kalyanaraman B, Malecki JG, Zielonka J, Podsiadły R. On the Chemical Reactivity of Tricyanofuran(TCF)-Based Near-Infrared Fluorescent Redox Probes – Effects of Glutathione on the Probe Response and Product Fluorescence. Dyes and Pigments. 2021;192:109405.

Chelimsky G, Simpson P, Zhang L, Bierer D, Komas S, Kalyanaraman B, Chelimsky T. Impaired Mitochondrial Bioenergetics Function in Pediatric Chronic Overlapping Pain Conditions with Functional Gastrointestinal Disorders. Pain Res Manag. 2021 Aug 13; 2021:6627864. PMID: 34426756; PMCID: PMC8380178

Modrzejewska J, Szala M, Grzelakowska A, Zakłos-Szyda M, Zielonka J, Podsiadły R. Novel Boronate Probe Based on 3-Benzothiazol-2-yl-7-hydroxy-chromen-2-one for the Detection of Peroxynitrite and Hypochlorite. Molecules. 2021 Sep 30; 26(19):5940. PMID: 34641484; PMCID: PMC8512868

Palma FR, Ratti BA, Paviani V, Coelho DR, Miguel R, Danes JM, Zaichik SV, de Abreu AL, Silva SO, Chen Y, Silverstein RL, Karan U, Jones DP, Bonini MG. AMPK-Deficiency Forces Metformin-Challenged Cancer Cells to Switch from Carbohydrate Metabolism to Ketogenesis to Support Energy Metabolism. Oncogene. 2021 Sep;40(36):5455-5467. PMID: 34290400


Dye Technology Team at the Institute of Polymer and Dye Technology, Lodz University of Technology. Peroxy-caged luciferin (PCL-1) probe for peroxynitrite detection. Application of Triple Quad LC-MS for detection of luciferin-6’-p-nitrobenzyl ether – a footprint of peroxynitrite. Shimadzu News 2020.

Mokry RL, Schumacher ML, Hogg N, Terhune SS. Nitric Oxide Circumvents Virus-Mediated Metabolic Regulation during Human Cytomegalovirus Infection. mBio. 2020 Dec 15;11(6):e02630-20. doi: 10.1128/mBio.02630-20. PMID: 33323506; PMCID: PMC7773989.

Szala M, Grzelakowska A, Modrzejewska J, Siarkiewicz P, Słowiński D, Świerczyńska M, Zielonka J, Podsiadły R. Characterization of the reactivity of luciferin boronate - A probe for inflammatory oxidants with improved stability. Dyes and Pigments. 2020 Dec;183:108693. doi: 10.1016/j.dyepig.2020.108693.

Angireddy R, Chowdhury AR, Zielonka J, Ruthel G, Kalyanaraman B, Avadhani NG. Alcohol-induced CYP2E1, mitochondrial dynamics and retrograde signaling in human hepatic 3D organoids. Free Radic Biol Med. 2020 Nov 1;159:1-14. doi: 10.1016/j.freeradbiomed.2020.06.030. PMID: 32738395.

Arzua T, Yan Y, Jiang C, Logan S, Allison RL, Wells C, Kumar SN, Schäfer R, Bai X. Modeling alcohol-induced neurotoxicity using human induced pluripotent stem cell-derived three-dimensional cerebral organoids. Transl Psychiatry. 2020 Oct 13;10(1):347. doi: 10.1038/s41398-020-01029-4. Erratum in: Transl Psychiatry. 2021 Feb 1;11(1):87. PMID: 33051447; PMCID: PMC7553959.

Podsiadly R, Grzelakoswaka A, Kolinska J, Zielonka J. Triple Quad LC-MS for detection of luciferin-6'-p-nitrobenzyl ether - a footprint of peroxynitrite. Analytik News. 2020 Oct 1. 1-3. [technical paper]

Michalski R, Thiebaut D, Michałowski B, Ayhan MM, Hardy M, Ouari O, Rostkowski M, Smulik-Izydorczyk R, Artelska A, Marcinek A, Zielonka J, Kalyanaraman B, Sikora A. Oxidation of ethidium-based probes by biological radicals: mechanism, kinetics and implications for the detection of superoxide. Sci Rep. 2020 Oct 29;10(1):18626. doi: 10.1038/s41598-020-75373-2. PMID: 33122809; PMCID: PMC7596101.

Cheng G, Hardy M, Topchyan P, Zander R, Volberding P, Cui W, Kalyanaraman B. Potent inhibition of tumour cell proliferation and immunoregulatory function by mitochondria-targeted atovaquone. Sci Rep. 2020 Oct 21;10(1):17872. doi: 10.1038/s41598-020-74808-0. PMID: 33087770.

Emelyanova L, Bai X, Yan Y, Bosnjak ZJ, Kress D, Warner C, Kroboth S, Rudic T, Kaushik S, Stoeckl E, Ross GR, Rizvi F, Jamil Tajik A, Jahangir A. Biphasic effect of metformin on human cardiac energetics. Transl Res. 2020 Oct 9:S1931-5244(20)30239-5. doi: 10.1016/j.trsl.2020.10.002. PMID: 33045408.

Yang M, Sun J, Stowe DF, Tajkhorshid E, Kwok WM, Camara AKS. Knockout of VDAC1 in H9c2 Cells Promotes Oxidative Stress-Induced Cell Apoptosis through Decreased Mitochondrial Hexokinase II Binding and Enhanced Glycolytic Stress. Cell Physiol Biochem. 2020 Sep 9;54(5):853-874. doi: 10.33594/000000274. PMID: 32901466.

Sikora A, Zielonka J, Debowska K, Michalski R, Smulik-Izydorczyk R, Pieta J, Podsiadly R, Artelska A, Pierzchala K, Kalyanaraman B. Boronate-based probes for biological oxidants: A novel class of molecular tools for redox biology. Front Chem. 2020 Sep;8:580899. doi: 10.3389/fchem.2020.580899. PMID: 33102447; PMCID: PMC7545953.

Cheng G, Hardy M, Zielonka J, Weh K, Zielonka M, Boyle KA, Abu Eid M, McAllister D, Bennett B, Kresty LA, Dwinell MB, Kalyanaraman B. Mitochondria-targeted magnolol inhibits OXPHOS, proliferation, and tumor growth via modulation of energetics and autophagy in melanoma cells. Cancer Treat Res Commun. 2020 Sep 17;25:100210. doi: 10.1016/j.ctarc.2020.100210. PMID: 32987287.

Yang M, Li W, Harberg C, Chen W, Yue H, Ferreira RB, Wynia-Smith SL, Carroll KS, Zielonka J, Flaumenhaft R, Silverstein RL, Smith BC. Cysteine sulfenylation by CD36 signaling promotes arterial thrombosis in dyslipidemia. Blood Adv. 2020 Sep 22;4(18):4494-4507. doi: 10.1182/bloodadvances.2020001609. PMID: 32946569; PMCID: PMC7509873.

Kalyanaraman B. Teaching the basics of repurposing mitochondria-targeted drugs: From Parkinson's disease to cancer and back to Parkinson's disease. Redox Biol. 2020 Sep;36:101665. doi: 10.1016/j.redox.2020.101665. PMID: 32795938; PMCID: PMC7426584.

Chowdhury AR, Zielonka J, Kalyanaraman B, Hartley RC, Murphy MP, Avadhani NG. Mitochondria-targeted paraquat and metformin mediate ROS production to induce multiple pathways of retrograde signaling: A dose-dependent phenomenon. Redox Biol. 2020 Sep;36:101606. doi: 10.1016/j.redox.2020.101606. PMID: 32604037; PMCID: PMC7327929.

Wang F, Qi XM, Wertz R, Mortensen M, Hagen C, Evans J, Sheinin Y, James M, Liu P, Tsai S, Thomas J, Mackinnon A, Dwinell M, Myers CR, Bartrons Bach R, Fu L, Chen G. p38γ MAPK is essential for aerobic glycolysis and pancreatic tumorigenesis. Cancer Res. 2020 Aug 15;80(16):3251-3264. doi: 10.1158/0008-5472.CAN-19-3281. PMID: 32580961.

Rios N, Radi R, Kalyanaraman B, Zielonka J. Tracking isotopically labeled oxidants using boronate-based redox probes. J Biol Chem. 2020 May 8;295(19):6665-6676. doi: 10.1074/jbc.RA120.013402. PMID: 32217693; PMCID: PMC7212653.

Zhang Q, Cheng G, Pan J, Zielonka J, Xiong D, Myers CR, Feng L, Shin SS, Kim YH, Bui D, Hu M, Bennett B, Schmainda K, Wang Y, Kalyanaraman B, You M. Magnolia extract is effective for the chemoprevention of oral cancer through its ability to inhibit mitochondrial respiration at complex I. Cell Commun Signal. 2020 Apr 7;18(1):58. doi: 10.1186/s12964-020-0524-2. PMID: 32264893; PMCID: PMC7140380.

Cheng G, Pan J, Podsiadly R, Zielonka J, Garces AM, Dias Duarte Machado LG, Bennett B, McAllister D, Dwinell MB, You M, Kalyanaraman B. Increased formation of reactive oxygen species during tumor growth: Ex vivo low-temperature EPR and in vivo bioluminescence analyses. Free Radic Biol Med. 2020 Feb 1;147:167-174. doi: 10.1016/j.freeradbiomed.2019.12.020. PMID: 31874251; PMCID: PMC6948008.


Nasci VL, Chuppa S, Griswold L, Goodreau KA, Dash RK, Kriegel AJ. miR-21-5p regulates mitochondrial respiration and lipid content in H9C2 cells. Am J Physiol Heart Circ Physiol. 2019 Mar 1;316(3):H710-H721. doi: 10.1152/ajpheart.00538.2017. PMID: 30657727, PMCID: PMC6459316

Cheng G, Zhang Q, Pan J, Lee Y, Ouari O, Hardy M, Zielonka M, Myers CR, Zielonka J, Weh K, Chang AC, Chen G, Kresty L, Kalyanaraman B, You M. Targeting lonidamine to mitochondria mitigates lung tumorigenesis and brain metastasis. Nat Commun. 2019 May;10(1):2205. doi: 10.1038/s41467-019-10042-1. PMID: 31101821, PMCID: PMC6525201

Cheng G, Zielonka J, Hardy M, Ouari O, Chitambar CR, Dwinell MB, Kalyanaraman B. Synergistic inhibition of tumor cell proliferation by metformin and mito-metformin in the presence of iron chelators. Oncotarget. 2019 May;10(37):3518-3532. doi: 10.18632/oncotarget.26943. PMID: 31191823, PMCID: PMC6544408

Zielonka J, Zielonka M, Cheng G, Hardy M, Kalyanaraman B. High-throughput screening of NOX inhibitors. Methods Mol Biol. 2019;1982:429-446. doi: 10.1007/978-1-4939-9424-3_25. PMID: 31172487

Zielonka J, Zielonka M, Kalyanaraman B. HPLC-based monitoring of oxidation of hydroethidine for the detection of NADPH oxidase-derived superoxide radical anion. Methods Mol Biol. 2019;1982:243-258. doi: 10.1007/978-1-4939-9424-3_14. PMID: 31172476

Horikoshi Y, Yan Y, Terashvili M, Wells C, Horikoshi H, Fujita S, Bosnjak ZJ, Bai X. Fatty acid-treated induced pluripotent stem cell-derived human cardiomyocytes exhibit adult cardiomyocyte-like energy metabolism phenotypes. Cells. 2019 Sep;8(9). pii: E1095. doi: 10.3390/cells8091095. PMID: 31533262, PMCID: PMC6769886

Emelyanova L, Sra A, Schmuck EG, Raval AN, Downey FX, Jahangir A, Rizvi F, Ross GR. Impact of statins on cellular respiration and de-differentiation of myofibroblasts in human failing hearts. ESC Heart Fail. 2019 Oct;6(5):1027-1040. doi: 10.1002/ehf2.12509. PMID: 31520523, PMCID: PMC6816080

He C, Danes JM, Hart PC, Zhu Y, Huang Y, de Abreu AL, O'Brien J, Mathison AJ, Tang B, Frasor JM, Wakefield LM, Ganini D, Stauder E, Zielonka J, Gantner BN, Urrutia RA, Gius D, Bonini MG. SOD2 acetylation on lysine 68 promotes stem cell reprogramming in breast cancer. Proc Natl Acad Sci U S A. 2019 Nov 19;116(47):23534-23541. doi: 10.1073/pnas.1902308116. PMID: 3159107, PMCID: PMC6876149

Chen Y, Yang M, Huang W, Chen W, Zhao Y, Schulte ML, Volberding P, Gerbec Z, Zimmermann MT, Zeighami A, Demos W, Zhang J, Knaack DA, Smith BC, Cui W, Malarkannan S, Sodhi K, Shapiro JI, Xie Z, Sahoo D, Silverstein RL. Mitochondrial metabolic reprogramming by CD36 signaling drives macrophage inflammatory responses. Circ Res. 2019 Dec 6;125(12):1087-1102. doi: 10.1161/CIRCRESAHA.119.315833. PMID: 31625810, PMCID: PMC6921463

Cheng G, Pan J, Podsiadly R, Zielonka J, Garces AM, Dias Duarte Machado LG, Bennett B, McAllister D, Dwinell MB, You M, Kalyanaraman B. Increased formation of reactive oxygen species during tumor growth: Ex vivo low-temperature EPR and in vivo bioluminescence analyses. Free Radic Biol Med. 2019 Dec 23;147:167-174. doi: 10.1016/j.freeradbiomed.2019.12.020. PMID: 31874251


Cheng G, Zielonka M, Dranka B, Kumar SN, Myers CR, Bennett B, Garces AM, Dias Duarte Machado LG, Thiebaut D, Ouari O, et al. Detection of mitochondria-generated reactive oxygen species in cells using multiple probes and methods: Potentials, pitfalls, and the future. J Biol Chem. 2018. pii: jbc.RA118.003044. doi: 10.1074/jbc.RA118.003044. [Epub ahead of print] PubMed PMID: 29739855

Pan J, Lee Y, Cheng G, Zielonka J, Zhang Q, Bajzikova M, Xiong D, Tsaih S-W, Hardy M, Flister M, Olsen CM, Wang Y, Vang O, Neuzil J, Myers CR, Kalyanaraman B, You M. Mitochondria-targeted honokiol confers a striking inhibitory effect on lung cancer via inhibiting Complex I activity. iScience. 2018;3:192-207. doi: 10.1016/j.isci.2018.04.013.

Xu Z, Ni B, Cao Z, Zielonka J, Gao J, Chen F, Kalyanaraman B, White GC, Ma YQ. Kindlin-3 negatively regulates the release of neutrophil extracellular traps. J Leukoc Biol. 2018.

Chitambar CR, Al-Gizawiy MM, Alhajala HS, Pechman KR, Wereley JP, Wujek R, Clark PA, Kuo JS, Antholine WE, Schmainda KM. Gallium maltolate disrupts tumor iron metabolism and retards the growth of glioblastoma by inhibiting mitochondrial function and ribonucleotide reductase. Mol Cancer Ther. 2018 Mar 28. doi: 10.1158/1535-7163.MCT-17-1009. [Epub ahead of print] PubMed PMID: 29592883

Pan J, Lee Y, Cheng G, Zielonka J, Zhang Q, Bajzikova M, Xiong D, Tsaih SW, Hardy M, Flister M, Olsen CM. Mitochondria targeted honokiol confer a striking inhibitory effect on lung cancer via inhibiting complex I activity. iScience. 2018.

Zielonka J, Kalyanaraman B. Small-molecule luminescent probes for the detection of cellular oxidizing and nitrating species. Free Radic Biol Med. 2018 Mar 19. pii: S0891-5849(18)30135-7. doi: 10.1016/j.freeradbiomed.2018.03.032. [Epub ahead of print] Review. PubMed PMID: 29567392.

Kalyanaraman B, Cheng G, Hardy M, Ouari O, Lopez M, Joseph J, Zielonka J, Dwinell MB. A review of the basics of mitochondrial bioenergetics, metabolism, and related signaling pathways in cancer cells: Therapeutic targeting of tumor mitochondria with lipophilic cationic compounds. Redox Biol. 2018;14:316-27. Epub 2017/10/11. doi: 10.1016/j.redox.2017.09.020. PubMed PMID: 29017115; PMCID: PMC5633086.

Hardy M, Zielonka J, Karoui H, Sikora A, Michalski R, Podsiadly R, Lopez M, Vasquez-Vivar J, Kalyanaraman B, Ouari O. Detection and Characterization of Reactive Oxygen and Nitrogen Species in Biological Systems by Monitoring Species-Specific Products. Antioxid Redox Signal. 2017. Epub 2017/10/19. doi: 10.1089/ars.2017.7398. PubMed PMID: 29037049.

Kalyanaraman B, Cheng G, Hardy M, Ouari O, Bennett B, Zielonka J. Teaching the basics of reactive oxygen species and their relevance to cancer biology: Mitochondrial reactive oxygen species detection, redox signaling, and targeted therapies. Redox Biology. 2018;15:347-62. doi: PMCID: PMC5756055

Boyle KA, Van Wickle J, Hill RB, Marchese A, Kalyanaraman B, Dwinell MB. Mitochondria-targeted drugs stimulate mitophagy and abrogate colon cancer cell proliferation. J Biol Chem. 2018 Sep 21;293(38):14891-14904. doi: 10.1074/jbc.RA117.001469.

Zielonka J, Hardy M, Michalski R, Sikora A, Zielonka M, Cheng G, Ouari O, Podsiadły R, Kalyanaraman B. Recent Developments in the Probes and Assays for Measurement of the Activity of NADPH Oxidases. Cell Biochem Biophys. 2017 Jun 29. doi: 10.1007/s12013-017-0813-6. [Epub ahead of print] PubMed PMID: 28660426

Zielonka J, Joseph J, Sikora A, Hardy M, Ouari O, Vasquez-Vivar J, Cheng G, Lopez M, Kalyanaraman B. Mitochondria-Targeted Triphenylphosphonium-Based Compounds: Syntheses, Mechanisms of Action, and Therapeutic and Diagnostic Applications. Chem Rev. 2017 Jun 27. doi: 10.1021/acs.chemrev.7b00042. [Epub ahead of print] PubMed PMID: 28654243

Starenki D, Hong SK, Wu PK, Park JI. Vandetanib and cabozantinib potentiate mitochondria-targeted agents to suppress medullary thyroid carcinoma cells. Cancer Biol Ther. 2017 May 5:1-11. doi: 10.1080/15384047.2017.1323594. [Epub ahead of print] PubMed PMID: 28475408

Egea J, Fabregat I, Frapart YM, Ghezzi P, Görlach A, Kietzmann T, Kubaichuk K, Knaus UG, Lopez MG, Olaso-Gonzalez G, Petry A, Schulz R, Vina J, Winyard P, Abbas K, Ademowo OS, Afonso CB, Andreadou I, Antelmann H, Antunes F, Aslan M, Bachschmid MM, Barbosa RM, Belousov V, Berndt C, Bernlohr D, Bertrán E, Bindoli A, Bottari SP, Brito PM, Carrara G, Casas AI, Chatzi A, Chondrogianni N, Conrad M, Cooke MS, Costa JG, Cuadrado A, My-Chan Dang P, De Smet B, Debelec-Butuner B, Dias IHK, Dunn JD, Edson AJ, El Assar M, El-Benna J, Ferdinandy P, Fernandes AS, Fladmark KE, Förstermann U, Giniatullin R, Giricz Z, Görbe A, Griffiths H, Hampl V, Hanf A, Herget J, Hernansanz-Agustín P, Hillion M, Huang J, Ilikay S, Jansen-Dürr P, Jaquet V, Joles JA, Kalyanaraman B, Kaminskyy D, Karbaschi M, Kleanthous M, Klotz LO, Korac B, Korkmaz KS, Koziel R, Kračun D, Krause KH, Křen V, Krieg T, Laranjinha J, Lazou A, Li H, Martínez-Ruiz A, Matsui R, McBean GJ, Meredith SP, Messens J, Miguel V, Mikhed Y, Milisav I, Milković L, Miranda-Vizuete A, Mojović M, Monsalve M, Mouthuy PA, Mulvey J, Münzel T, Muzykantov V, Nguyen ITN, Oelze M, Oliveira NG, Palmeira CM, Papaevgeniou N, Pavićević A, Pedre B, Peyrot F, Phylactides M, Pircalabioru GG, Pitt AR, Poulsen HE, Prieto I, Rigobello MP, Robledinos-Antón N, Rodríguez-Mañas L, Rolo AP, Rousset F, Ruskovska T, Saraiva N, Sasson S, Schröder K, Semen K, Seredenina T, Shakirzyanova A, Smith GL, Soldati T, Sousa BC, Spickett CM, Stancic A, Stasia MJ, Steinbrenner H, Stepanić V, Steven S, Tokatlidis K, Tuncay E, Turan B, Ursini F, Vacek J, Vajnerova O, Valentová K, Van Breusegem F, Varisli L, Veal EA, Yalçın AS, Yelisyeyeva O, Žarković N, Zatloukalová M, Zielonka J, Touyz RM, Papapetropoulos A, Grune T, Lamas S, Schmidt HHHW, Di Lisa F, Daiber A. European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS). Redox Biol. 2017 May 18;13:94-162. doi: 10.1016/j.redox.2017.05.007. [Epub ahead of print] Review. PubMed PMID: 28577489; PubMed Central PMCID: PMC5458069

Kalyanaraman B, Cheng G, Hardy M, Ouari O, Sikora A, Zielonka J, Dwinell MB. Modified Metformin as a More Potent Anticancer Drug: Mitochondrial Inhibition, Redox Signaling, Antiproliferative Effects and Future EPR Studies. Cell Biochem Biophys. 2017 Apr 21. doi: 10.1007/s12013-017-0796-3. [Epub ahead of print] PubMed PMID: 28429253

Kalyanaraman B, Cheng G, Hardy M, Ouari O, Sikora A, Zielonka J, Dwinell M. Mitochondria-targeted metformins: anti-tumour and redox signalling mechanisms. Interface Focus. 2017 Apr 6;7(2):20160109. doi: 10.1098/rsfs.2016.0109. Review. PubMed PMID: 28382202; PubMed Central PMCID: PMC5311906.

Langley M, Ghosh A, Charli A, Sarkar S, Ay M, Luo J, Zielonka J, Brenza T, Bennett B, Jin H, Ghaisas S, Schlichtmann B, Kim D, Anantharam V, Kanthasamy A, Narasimhan B, Kalyanaraman B, Kanthasamy AG. Mito-Apocynin Prevents Mitochondrial Dysfunction, Microglial Activation, Oxidative Damage, and Progressive Neurodegeneration in MitoPark Transgenic Mice. Antioxid Redox Signal. 2017;27(14):1048-66. Epub 2017/04/05. doi: 10.1089/ars.2016.6905. PubMed PMID: 28375739; PMCID: PMC5651937.

Hong SK, Starenki D, Wu PK, Park JI. Suppression of B-Raf(V600E) melanoma cell survival by targeting mitochondria using triphenyl-phosphonium-conjugated nitroxide or ubiquinone. Cancer biology & therapy. 2017;18(2):106-14. Epub 2016/10/28. doi: 10.1080/15384047.2016.1250987. PubMed PMID: 27786591; PMCID: PMC5362989.
Cheng G, Zielonka J, Ouari O, Lopez M, McAllister D, Boyle K, Barrios CS, Weber JJ, Johnson BD, Hardy M, et al. Mitochondria-Targeted Analogues of Metformin Exhibit Enhanced Antiproliferative and Radiosensitizing Effects in Pancreatic Cancer Cells. Cancer Res. 2016 Jul 1;76(13):3904-15.

Zielonka J, Zielonka M, VerPlank L, Cheng G, Hardy M, Ouari O, Ayhan MM, Podsiadly R, Sikora A, Lambeth JD, Kalyanaraman B. Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit Peroxynitrite Formation. J Biol Chem. 2016; 291:7029-44.

Debski D, Smulik R, Zielonka J, Michalowskie B, Jakubowska M, Debowska K, Adamus J, Marcinek A, Kalyanaraman B, Kisora A. Mechanism of oxidative conversion of Amplex® Red to resorufin: pulse radiolysis and enzymatic studies. Free Radic Biol Med. 2016; 95:323-32.

Kalyanaraman B, Hardy M, Zielonka J. A Critical Review of Methodologies to Detect Reactive Oxygen and Nitrogen Species Stimulated by NADPH Oxidase Enzymes: Implications in Pesticide Toxicity. Curr Pharmacol Rep. 2016; 2:193.

Zielonka J, Podsiadly R, Zielonka M, Hardy M, Kalyanaraman B. On the use of peroxy-caged luciferin (PCL-1) probe for bioluminescent detection of inflammatory oxidants in vitro and in vivo - Identification of reaction intermediates and oxidant-specific minor products. Free Radic Biol Med. 2016; 99:32-42.

Kalyanaraman B, Hardy M, Podsiadly R, Cheng G, Zielonka J. Recent developments in detection of superoxide radical anion and hydrogen peroxide: Opportunities, challenges, and implications in redox signaling. Arch Biochem Biophys. 2016; 617:38-47.

Kutty RG, Gang X, Schauder DM, Cossette SM, Bordas M, Cui W, Ramchandran R. Dual Specificity Phosphatase 5 Is Essential for T Cell Survival. PLOS ONE 11, no. 12 (2016): e0167246.

Myers CR. Enhanced targeting of mitochondrial peroxide defense by the combined use of thiosemicarbazones and inhibitors of thioredoxin reductase. Free radical biology & medicine. 2016;91:81-92. Epub 2015/12/22. doi: 10.1016/j.freeradbiomed.2015.12.008. PubMed PMID: 26686468

Zielonka, J., Sikora, A., Adamus, J. and Kalyanaraman, B., 2015. Detection and differentiation between peroxynitrite and hydroperoxides using mitochondria-targeted arylboronic acid. Mitochondrial Medicine: Volume I, Probing Mitochondrial Function, pp.171-181.

Cheng G, Zielonka J, McAllister D, Hardy M, Ouari O, Joseph J, Dwinell MB, Kalyanaraman B. Antiproliferative effects of mitochondria-targeted cationic antioxidants and analogs: Role of mitochondrial bioenergetics and energy-sensing mechanism. Cancer Lett 365:96-106, 2015.

Kittipongdaja W, Wu X, Garner J, Liu X, Komas SM, Hwang ST, Schieke SM. Rapamycin Suppresses Tumor Growth and Alters the Metabolic Phenotype in T-Cell Lymphoma. J Invest Dermatol 135:2301–8, 2015.

Zhang Q, Pan J, Lubet RA, Komas SM, Kalyanaraman B, Wang Y, You M. Enhanced Antitumor activity of 3-bromopyruvate in combination with rapamycin in vivo and in vitro. Cancer Prev Res (Phila) 8:318–26, 2015.

Roy I, McAllister DM, Gorse E, Dixon K, Piper CT, Zimmerman NP, Getschman AE, Tsai S, Engle DD, Evans D, Volkman BF, Kalyanaraman B, Dwinell MB. Chemokine biased agonism regulates pancreatic cancer migration and metastasis through bioenergetics signaling. Cancer Res 75(17):3529-42, 2015.

Debowska K, Debski D, Hardy M, Jakubowska M, Kalyanaraman B, Marcinek A, Michalski R, Michalowski B, Ouari O, Sikora A, Smulik R, Zielonka J. Toward selective detection of reactive oxygen and nitrogen species with the use of fluorogenic probes--Limitations, progress, and perspectives. Pharmacol Rep 67(4)756-64, 2015.

Starenki D, Hong SK, Lloyd RV, Park JI. Mortalin (GRP75/HSPA9) upregulation promotes survival and proliferation of medullary thyroid carcinoma cells. Oncogene. 34(35):4624-34, 2015. PMCID: PMC4451452.

Smulik, R., Dębski, D., Zielonka, J., Michałowski, B., Adamus, J., Marcinek, A., Kalyanaraman, B. and Sikora, A., 2014. Nitroxyl (HNO) reacts with molecular oxygen and forms peroxynitrite at physiological pH biological implications. Journal of Biological Chemistry, 289(51), pp.35570-35581.

Shah, G., Zielonka, J., Chen, F., Zhang, G., Cao, Y., Kalyanaraman, B. and See, W., 2014. H 2 O 2 Generation by bacillus Calmette-Guérin Induces the Cellular Oxidative Stress Response Required for bacillus Calmette-Guérin Direct Effects on Urothelial Carcinoma Biology. The Journal of urology, 192(4), pp.1238-1248.

Michalski, R., Zielonka, J., Gapys, E., Marcinek, A., Joseph, J. and Kalyanaraman, B., 2014. Real-time measurements of amino acid and protein hydroperoxides using coumarin boronic acid. Journal of Biological Chemistry, 289(32), pp.22536-22553.

Hardy, M., Poulhés, F., Rizzato, E., Rockenbauer, A., Banaszak, K., Karoui, H., Lopez, M., Zielonka, J., Vasquez-Vivar, J., Sethumadhavan, S. and Kalyanaraman, B., 2014. Mitochondria-targeted spin traps: synthesis, superoxide spin trapping, and mitochondrial uptake. Chemical research in toxicology, 27(7), pp.1155-1165.

Koto, T., Michalski, R., Zielonka, J., Joseph, J. and Kalyanaraman, B., 2014. Detection and identification of oxidants formed during NO/O2–reaction: A multi-well plate CW-EPR spectroscopy combined with HPLC analyses. Free radical research, 48(4), pp.478-486.

Kalyanaraman, B., Dranka, B.P., Hardy, M., Michalski, R. and Zielonka, J., 2014. HPLC-based monitoring of products formed from hydroethidine-based fluorogenic probes—the ultimate approach for intra-and extracellular superoxide detection. Biochimica et Biophysica Acta (BBA)-General Subjects, 1840(2), pp.739-744.

Michalski, R., Michalowski, B., Sikora, A., Zielonka, J. and Kalyanaraman, B., 2014. On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: a reassessment. Free Radical Biology and Medicine, 67, pp.278-284.

Pan J., Zhang Q., Liu Q., Komas S.M., Kalyanaraman B., Lubet R.A., Wang Y., You M. Honokiol inhibits lung tumorigenesis through inhibition of mitochondrial function. Cancer Prev Res (Phila) 7:1149–59, 2014.

Cheng G., Zielonka J., McAllister D., Tsai S., Dwinell M.B., Kalyanaraman B. Profiling and targeting of cellular bioenergetics: inhibition of pancreatic cancer cell proliferation. Br J Cancer 111:85–93, 2014.

Mouradian M., Kikawa K.D., Dranka B.P., Komas S.M., Kalyanaraman B. and Pardini R.S. (2014). Docosahexaenoic acid attenuates breast cancer cell metabolism and the Warburg phenotype by targeting bioenergetic function. Mol. Carcinog. doi: 10.1002/mc.2215

Zielonka J., Cheng G., Zielonka M., Ganesh T., Sun A., Joseph J., Michalski R., O'Brien W.J., Lambeth J.D., Kalyanaraman B. (2014). High-throughput Assays for Superoxide and Hydrogen Peroxide: Design of a Screening Workflow to Identify Inhibitors of NADPH Oxidases. J Biol Chem. 289(23):16176-89.

Matthew D. Greseth and Paula Traktman (2014). De novo Fatty Acid Biosynthesis Contributes Significantly to Establishment of a Bioenergetically Favorable Environment for Vaccinia Virus Infection. PLoS Pathog.

Cheng G., Zielonka J., McAllister D., Tsai S., Dwinell M.B., and Kalyanaraman B. (2014). Profiling and targeting of cellular bioenergetics: inhibition of pancreatic cancer cell proliferation. British Journal of Cancer (2014) 111, 85–93.

Zielonka, J., Lambeth, J.D. and Kalyanaraman, B., 2013. On the use of L-012, a luminol-based chemiluminescent probe, for detecting superoxide and identifying inhibitors of NADPH oxidase: a reevaluation. Free Radical Biology and Medicine, 65, pp.1310-1314.

Dickey, J.S., Gonzalez, Y., Aryal, B., Mog, S., Nakamura, A.J., Redon, C.E., Baxa, U., Rosen, E., Cheng, G., Zielonka, J. and Parekh, P., 2013. Mito-tempol and dexrazoxane exhibit cardioprotective and chemotherapeutic effects through specific protein oxidation and autophagy in a syngeneic breast tumor preclinical model. PLoS One, 8(8), p.e70575.

Sikora, A., Zielonka, J., Adamus, J., Debski, D., Dybala-Defratyka, A., Michalowski, B., Joseph, J., Hartley, R.C., Murphy, M.P. and Kalyanaraman, B., 2013. Reaction between peroxynitrite and triphenylphosphonium-substituted arylboronic acid isomers: identification of diagnostic marker products and biological implications. Chemical research in toxicology, 26(6), pp.856-867.

Michalski, R., Zielonka, J., Hardy, M., Joseph, J. and Kalyanaraman, B., 2013. Hydropropidine: a novel, cell-impermeant fluorogenic probe for detecting extracellular superoxide. Free Radical Biology and Medicine, 54, pp.135-147.

Zielonka, J., Joseph, J., Sikora, A. and Kalyanaraman, B., 2013. Real-time monitoring of reactive oxygen and nitrogen species in a multiwell plate using the diagnostic marker products of specific probes. Methods Enzymol, 526, pp.145-157.

Starenki, D. and Park, J.I., 2013. Mitochondria-targeted nitroxide, Mito-CP, suppresses medullary thyroid carcinoma cell survival in vitro and in vivo. The Journal of Clinical Endocrinology & Metabolism, 98(4), pp.1529-1540.

Cheng, G., Zielonka, J., McAllister, D.M., Mackinnon, A.C., Joseph, J., Dwinell, M.B. and Kalyanaraman, B., 2013. Mitochondria-targeted vitamin E analogs inhibit breast cancer cell energy metabolism and promote cell death. BMC cancer, 13(1), p.285.

Dranka, B.P., Gifford, A., Ghosh, A., Zielonka, J., Joseph, J., Kanthasamy, A.G. and Kalyanaraman, B., 2013. Diapocynin prevents early Parkinson's disease symptoms in the leucine-rich repeat kinase 2 (LRRK2 R1441G) transgenic mouse. Neuroscience letters, 549, pp.57-62.

View the CCBSR Information Poster (PDF)

Zielonka, J., Sikora, A., Hardy, M., Joseph, J., Dranka, B.P. and Kalyanaraman, B., 2012. Boronate probes as diagnostic tools for real time monitoring of peroxynitrite and hydroperoxides. Chemical research in toxicology, 25(9), pp.1793-1799.

Zielonka, J., Zielonka, M., Sikora, A., Adamus, J., Joseph, J., Hardy, M., Ouari, O., Dranka, B.P. and Kalyanaraman, B., 2012. Global profiling of reactive oxygen and nitrogen species in biological systems high-throughput real-time analyses. Journal of Biological Chemistry, 287(5), pp.2984-2995.

Mukhopadhyay, P., Horváth, B., Zsengellér, Z., Zielonka, J., Tanchian, G., Holovac, E., Kechrid, M., Patel, V., Stillman, I.E., Parikh, S.M. and Joseph, J., 2012. Mitochondrial-targeted antioxidants represent a promising approach for prevention of cisplatin-induced nephropathy. Free Radical Biology and Medicine, 52(2), pp.497-506.

Dranka, B.P., Zielonka, J., Kanthasamy, A.G. and Kalyanaraman, B., 2012. Alterations in bioenergetic function induced by Parkinson’s disease mimetic compounds: lack of correlation with superoxide generation. Journal of neurochemistry, 122(5), pp.941-951.

Cheng, G., Zielonka, J., Dranka, B.P., McAllister, D., Mackinnon, A.C., Joseph, J. and Kalyanaraman, B., 2012. Mitochondria-targeted drugs synergize with 2-deoxyglucose to trigger breast cancer cell death. Cancer research, 72(10), pp.2634-2644.