Michael Widlansky Lab
Michael E. Widlansky, MD, MPH
Associate Professor, Department of Medicine
The Widlansky laboratory work is currently focused on the roles of mircroRNA-29, mitochondrial fission proteins, and the microbiome in the regulation of human vascular endothelial function. It also focuses on studying novel mechanisms of vascular dysfunction in diabetes. His work tests these novel mechanistic insights to determine their relevance to human vascular pathophysiology. In addition, the Widlansky laboratory studies the impact of dietary, pharmacological, and exercise-based interventions on vascular function persons with and without disease. The laboratory also has formed a human vascular research core to foster collaboration with investigators from other disciplines interested in the impact of vascular function on disease states relevant to their fields of interest.
Clinical Research Coordinator
My role in the Widlansky lab is to coordinate several research projects that explore endothelial dysfunction in individuals with Type 2 Diabetes and Coronary Artery Disease. This includes recruiting and consenting subjects, conducting study visits, and carrying out vascular tests. I preform a brachial artery ultrasound test known as Flow Mediated Dilation (FMD). The FMD provides the lab with valuable information into the pathology of endothelial dysfunction.
Mamatha Kakarla, PhD
Research Associate I
In Dr. Widlansky lab, I utilize different biochemical and molecular techniques and I work on different human endothelial cell lines. I conduct experiments to measure physiological levels of NO and levels of phospho e-NOS in cell cultures. I am involved in projects looking at factors affecting mitochondrial fission and endothelial dysfunction.
Venkata Krishna Puppala, MD, MPH
The focus of my research is endothelial function. Endothelial dysfunction within the endothelium has been linked to the development of atherosclerosis and increased risk of cardiovascular events. I am involved in a project where we are evaluating the role of derangements in the mitochondrial homeostasis leading to development of endothelial dysfunction in humans with longstanding type 2 diabetes. We study microvascular functions in humans by obtaining arterioles in subcutaneous fat via biopsies of subcutaneous adipose. In addition, I am interested in developing innovative methods for assessment of endothelial function in ambulatory setting.
Sudhi Tyagi, MD
Instructor, Postdoctoral Fellow
My research interests center on understanding mechanisms that lead to atherosclerotic heart disease and treatments that can halt its progression. I apply clinical-translational research methods including gluteal fat pad biopsies and arterial endothelial cell sampling to gain insight into disease mechanisms and possible treatment approaches.
Jingli Wang, MD, PhD
Research Scientist II
The interest of my study is related to vascular biology. The goal of my project is to reveal the pathophysiological relationship between derangements of endothelium-originated mitochondria and endothelial dysfunction in type 2 diabetes mellitus (T2DM) and insulin-induced hypoglycemia. Using basic laboratory techniques such as cell culture, video microscopy, fluorescence microscopy, confocal microscopy and tissue culture myograph, the biopsy specimens including arterioles and (mono-) lymphocytes from T2DM patients and non-T2DM patients are able to be extensively studied for understanding the pathogenesis of T2DM and hypoglycemia at the level of endothelium and mitochondria.
Rong Ying, MD
Research Technologist II
My work in Dr. Widlansky's lab is to study endothelial function in human arterioles and in cultured human endothelial cells. Through performing these studies, the mechanisms of vascular endothelial dysfunction caused by T2DM and insulin-induced hypoglycemia are being explored at the cellular and molecular levels. As a research technologist, I also help to maintain a human endothelial cell line for all investigators in the lab and to train students who are interested in vessel dissection and in vitro vessel study.
- Mobin Malik
- Tisha Suboc
- Mike Tanner
(Widlansky ME, Hill RB.) Transl Res. 2018 12;202:83-98.
(Malik M, Suboc TM, Tyagi S, Salzman N, Wang J, Ying R, Tanner MJ, Kakarla M, Baker JE, Widlansky ME.) Circ Res. 2018 Oct 12;123(9):1091-1102.
(Xue H, Zhang G, Geurts AM, Usa K, Jensen DM, Liu Y, Widlansky ME, Liang M.) EBioMedicine. 2018 Sep;35:260-269.
(Harwig MC, Viana MP, Egner JM, Harwig JJ, Widlansky ME, Rafelski SM, Hill RB.) Anal Biochem. 2018 Jul 01;552:81-99.
(Swartz AM, Cho CC, Welch WA, Widlansky ME, Maeda H, Strath SJ.) Am J Health Behav. 2018 May 01;42(3):90-101.
(Lobelo F, Rohm Young D, Sallis R, Garber MD, Billinger SA, Duperly J, Hutber A, Pate RR, Thomas RJ, Widlansky ME, McConnell MV, Joy EA, American Heart Association Physical Activity Committee of the Council on Lifestyle and Cardiometabolic Health; Council on Epidemiology and Prevention; Council on Clinical Cardiology; Council on Genomic and Precision Medicine; Council on Cardiovascular Surgery and Anesthesia; and Stroke Council.) Circulation. 2018 May 01;137(18):e495-e522.
(Widlansky ME, Jensen DM, Wang J, Liu Y, Geurts AM, Kriegel AJ, Liu P, Ying R, Zhang G, Casati M, Chu C, Malik M, Branum A, Tanner MJ, Tyagi S, Usa K, Liang M.) EMBO Mol Med. 2018 03;10(3).
(Kieu A, Shaikh A, Kaeppler M, Miles RJ, Widlansky ME.) J Am Soc Hypertens. 2018 02;12(2):108-116.
(Widlansky ME, Puppala VK, Suboc TM, Malik M, Branum A, Signorelli K, Wang J, Ying R, Tanner MJ, Tyagi S.) Vasc Med. 2017 06;22(3):189-196.
(Touyz RM, Montezano AC, Rios F, Widlansky ME, Liang M.) Circ Res. 2017 May 26;120(11):1721-1723.
(Dass N, Kilakkathi S, Obi B, Moosreiner A, Krishnaswami S, Widlansky ME, Kidambi S.) J Am Soc Hypertens. 2017 May;11(5):246-257.
(Tanner MJ, Wang J, Ying R, Suboc TB, Malik M, Couillard A, Branum A, Puppala V, Widlansky ME.) Am J Physiol Heart Circ Physiol. 2017 Mar 01;312(3):H515-H527.