Key Test Specifications
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microRNA and miRNA Sequencing
Sample preparation will enrich for small RNA
If total RNA is provided, input total RNA quality control with fragment analysis and quantification, library preparation (Captures small RNA with 3′ OH and 5′P modifications), Illumina sequencing. Recommended sequencing at 10 million reads per sample.
Total RNA, 1-1.5ug (at a concentration of at least 200ng/uL in nuclease free water); small RNA, 10-50ng (at a concentration of at least 2ng/uL in nuclease free water), quality and quantity of RNA will be verified in house prior to initiation of library preparation
Specimen Type and Requirements
Cells or fresh frozen tissue ** available with consultation; RNA extraction methods may be available for your cell or tissue type
Completed library prep
Sample numbers, adaptor sequence
Dry ice (-80C), overnight
If RNA or completed library prep is provided, 4-6 weeks; RNA extraction and optimization may require additional 1-2 weeks
Medical College of Wisconsin
8701 Watertown Plank Rd.
Milwaukee, WI 53226
(414) 955-6516 (fax)