Research Bench Lab

For Researchers

The Mellowes Center is dedicated to providing comprehensive data analytic solutions for a wide variety of researcher areas and experience levels. We deliver single- or multi-sample VCFs, tables, PDF reports, and/or new and interactive web-based reports, all using open-source data standards and multiple stages of quality control. Our interactive report advances beyond today's standards in baseline bioinformatics analysis. As your trusted partner, we are interested in developing custom novel analyses to meet your program's research and publication needs. Bioinformatics and Collaborative Science LabsSubmit a Research Consultation Form (DOCX)
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Key Test Specifications

Unsure what your project needs? Looking for a custom solution? Schedule a complimentary Project Consultation below. 
Whole Genome Sequencing (WGS)

Whole Genome Sequencing (WGS) is a method to comprehensively consider the DNA base pairs of a sample and evaluate variants that arise in the sequence.  The role of genomic variants in disease phenotypes and outcomes can be further investigated with expression, molecular modeling and epigenetic regulation studies.  The flexible method allows for sequencing of any species of interest including, but not limited to human, model organisms (mice, rat, etc.), plants, microbes and more.

Whole Exome Sequencing (WES)

Whole Exome Sequencing (WES) allows for the enrichment of ~2% of the human genome and focuses on the protein-coding regions that contain a large percentage of the known disease variants.  Focusing on a smaller sequencing region allows for greater depth and maintains a concentrated approach to understanding the role of variants in disease phenotypes.  Beyond categorization of variants with curated databases, research analysis can further investigate protein structure, dynamics, and function of the variant. 

RNA-Sequencing (RNA-Seq), Transcriptome

Transcriptomic Sequencing (RNA-Seq) provides scientists with an understanding of how a sample system responds to changes in conditions, treatments, gene variants, and a wide variety of other study designs.  Measuring gene expression changes (differential expression, DE) can provide understanding of cellular and molecular mechanisms that underly disease initiation, progression, response to environmental or therapeutic interventions and substantially more conditions. Sequencing the gene transcripts also produces information and insight into the expression of protein variants, transcript isoforms and novel fusion genes. 

MicroRNA Panel

MicroRNA (miRNA) plays an important role in controlling cellular gene expression and often create inhibitory feedback loops and act as negative regulators.  To reveal the miRNAs present in cellular systems, investigators can hybridize samples with an expression panel that identifies levels of up to 800 biologically relevant miRNA molecules (available as human, mouse or rat panels). 

Reduced Representation Bisulfide Sequencing (RRBS)

Reduced Representation Bisulfide Sequence (RRBS) seeks to understand the level of DNA methylation on cytosines as this an epigenetic mechanism for regulation of gene expression.  Through identification of methylation in regions of the genome with high CpG content (~1% of the genome), further understanding of the differential regulation of gene expression can be discovered in a variety of diseases, cancer, aging and many other experimental models.

Chromatin Immunoprecipitation Sequencing (ChIP-Seq)

Chromatin Immunoprecipitation Sequencing (ChIP-Seq) is a method to further understand the intersection of proteins (transcription factors or histones) with various regions of the human genome.  Understanding and mapping these interactions can reveal common binding sites and overall structural changes that can regulate the ultimate ability of the cell to express various genes. 

Bioinformatics Analysis Shared Resource

The Bioinformatics Analysis Shared Resource is dedicated to providing comprehensive solutions to a wide variety of research areas and experience levels. We deliver broad bioinformatic analysis for DNA, epigenetic, and RNA-based sequencing.  Our interactive web-based reports include technology-specific quality control metrics, multi-sample comparisons, and advanced analyses beyond current national core standards.  As your trusted partner, we consistently develop and apply custom analyses to meet your program's research and publication needs.

Schedule a Consultation

Contact Us

The Linda T. and John A. Mellowes Center for Genomic Sciences and Precision Medicine
Medical College of Wisconsin
8701 Watertown Plank Rd.
Milwaukee, WI 53226

(414) 955-4887
(414) 955-6516 (fax)

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