MCW Tissue Bank - Information For Researchers
Overview
The MCW Tissue Bank is a secure storage facility that collects, processes and distributes blood and tissue for research on campus.
The MCW Tissue Bank is a College of American Pathologists accredited biorepository. Our protocols and services are rigorously assessed and validated to ensure the highest standards are met.
Services
Histocytometry Services
Standard core-based tissue microarrays (TMA)
Tissue arrays by Cutting-Edge Matrix Assembly (CEMA)
Core-based cell pellet microarray
Tumor biomarker analytic support: Multicolor quantitative immunofluorescence histo-cytometry
MCW Tissue Bank Programs
General Tissue Bank
Consent Form
This is a lifetime consent and we consent for:
- One additional blood draw during a regularly scheduled clinic lab draw
- Otherwise discarded tissue from any future surgery, including bone marrow, tumor and control tissue
Availability to query available samples and deidentified clinical data with the help of CTSI’s i2b2 Cohort Discovery Tool. Deidentified clinical information we can provide:
- Race
- Age
- Pathological diagnosis
Participation Information
To participate in the MCW Tissue Bank subjects must:
- Be over 18 years of age
- Be a patient of Froedtert & the Medical College of Wisconsin
- Speak and read English (consent form is only available in English currently)
Maternal Research, Placenta & Cord Blood Bank
Consent Form
We consent each pregnancy for:
- Peripheral blood draw – usually takes place at approximately 20 weeks, but can be collected postpartum
- Cord blood at time of delivery
- Placenta and cord blood
Participation Information
To participate in the MCW Tissue Bank subjects must:
- Be over 18 years of age
- Be a patient delivering at Froedtert & the Medical College of Wisconsin
- Speak and read English (consent form is only available in English currently)
Immuno-Oncology Bone Marrow Bank
Consent Form
This is a lifetime consent, and we consent for:
- An additional bone marrow sample to be drawn for research purposes only, at any of the consented participants clinically ordered bone marrow biopsies or aspirates.
- A paired blood sample to be drawn for research purposes only at any regularly scheduled blood draw
Availability to query available samples and deidentified clinical data with the help of CTSI’s i2b2 Cohort Discovery Tool. De identified clinical information we can provide:
- Race
- Age
- Pathological diagnoses
Participation Information
To participate in the MCW Immuno-Oncology Bone Marrow Bank subjects must:
- Be 18 years of age or older
- Speak and read English (consent form is currently only available in English)
- Be a patient of Froedtert and the Medical College of Wisconsin
- Have a bone marrow biopsy or aspirate scheduled through Froedtert and the Medical College of Wisconsin
MCW Tissue Bank Request & Distribution Process
Request via iLab
All requests for MCW Tissue Bank services or samples should be made through iLab, which allows researchers to order, track, and pay for core services in a convenient, one-stop location. A fee schedule for MCW Tissue Bank services and samples can be found in iLab. To register your study in iLab, click the Register link below. Then complete and submit the Specimen/Service Request Form.
Committee Approval Process
Once your request has been made via iLab, the MCW Tissue Bank Manager will provide you with a quote for the services provided. When approved by the study team, the request is sent to the MCW Tissue Bank’s Utilization Committee, and if needed, the Executive Committee. Once the request is approved by Committee members, the decision will be communicated to the study PI.
Distribution Process
After Committee approval, MCW Tissue Bank staff will work on completing the study request. There is an approximate 2 week turnaround time for most requests.
Inquiry Form
If you are not ready to submit an iLab request and have general questions regarding MCW Tissue Bank services and samples, please fill out the Inquiry Form below. Inquires must be made by MCW faculty and their study team.
Publications
Keratinocytes contribute to normal cold and heat sensation. (Sadler KE, Moehring, F, Stucky CL.) Elife. 2020 Jul 30 PMID: 32729832 https://doi.org/10.7554/eLife.58625
Different Human Immune Lineage Compositions Are Generated in Non-Conditioned NBSGW Mice Depending on HSPC Source. (Hess N.J., Lindner P.N., Vazquez J., Grindel S., Hudson A.W., Stanic A.K., Ikeda A., Hematti P., Gumperz J.E.) Frontiers in Immunology. 2020 October 18 https://doi.org/10.3389/fimmu.2020.573406
GATA4, expressed in Barrett’s esophagus and esophageal adenocarcinoma, can block squamous epithelial cell gene expression in human esophageal cells. (Stavniichuk R, DeLaForest A., Thompson C.A., Miller J., Souza R.F., Battle M.A.) bioRxiv. 2020 June 18 https://doi.org/10.1101/2020.06.17.156026
H+/K+ATPase Expression in the Larynx of Laryngopharyngeal Reflux and Laryngeal Cancer Patients. (McCormick C.A., Samuels T.L., Battle M.A., Frolkis T., Blumin J.H., Bock J.M., Wells C., Yan K., Altman K.W., Johnston N.) Laryngoscope. 2020 April 06 https://doi.org/10.1002/lary.28643
Sensitive and specific immunohistochemistry protocols for detection of SARS-CoV-2 nucleocapsid and spike proteins in formalin-fixed, paraffin-embedded COVID-19 patient tissues. (Sun Y, Ge L, Rau MJ, et al. ) Research Square; 2020. https://doi.org/10.21203/rs.3.pex-1011/v1
Diffuse interstitial pneumonia-like/macrophage activation syndrome-like changes in patients with COVID-19 correlate with length of illness. (Felix J.C., Sheinin Y.M., Suster D., Ronen N., Ratiani M., Vanden Heuvel T., Winge E., Patton M.D., Rau M.J., Ge L., Sun Y., Udhane S.S., Langenheim J.F., Rui H.) Annals of Diagnostic Pathology. 2021 August. https://doi.org/10.1016/j.anndiagpath.2021.151744
Twist1 signaling in age-dependent decline in angiogenesis and lung regeneration. (Hendee K, Hunyenyiwa T, Matus K, Toledo M, Mammoto A, Mammoto T. ) Aging (Albany NY). 2021 Mar 25;13(6):7781-7799. Epub 2021 Mar 25. PMID: 33764901; PMCID: PMC8034921 https://doi.org/10.18632/aging.202875
GATA4 blocks squamous epithelial cell gene expression in human esophageal squamous cells. (Stavniichuk, R., DeLaForest, A., Thompson, C.A. et al.) Sci Rep 11, 3206 (2021). https://doi.org/10.1038/s41598-021-82557-x
Hypertension preserves the magnitude of microvascular flow-mediated dilation following transient elevation in intraluminal pressure. (Hughes W.E., Zinkevich N., Gutterman D.D., Beyer A.M.) Physiological Reports. 2021 February 15. https://doi.org/10.14814/phy2.14507
Pneumocytes are distinguished by highly elevated expression of the ER stress biomarker GRP78, a co-receptor for SARS-CoV-2, in COVID-19 autopsies. (Puzyrenko, A., Jacobs, E.R., Sun, Y. et al.) Cell Stress and Chaperones. 2021 August 12. https://doi.org/10.1007/s12192-021-01230-4
An Improved Detection of Circulating Tumor DNA in Extracellular Vesicles-Depleted Plasma. (Sun L., Du M., Kohli M., Huang C., Chen X., Xu M., Shen H., Wang S., Wang L.) Frontiers in Oncology. 2021 June 10. https://doi.org/10.3389/fonc.2021.691798
Targeted biologic inhibition of both tumor cell-intrinsic and intercellular CLPTM1L/CRR9-mediated chemotherapeutic drug resistance. (Parashar, D., Geethadevi, A., McAllister, D. et al. ) npj Precis. Onc. 5, 16 (2021). https://doi.org/10.1038/s41698-021-00152-9
Keratinocyte PIEZO1 modulates cutaneous mechanosensation. (Mikesell A.R., Isaeva O., Moehring F., Sadler K.E., Menzel A.D., Stucky C.L.) eLife. 2022 September 2. https://doi.org/10.7554/eLife.65987
Chronic stress promotes an immunologic inflammatory state and head and neck cancer growth in a humanized murine model. (Zenga J., Musaddiq A.J., Frei A., Petrie E., Sharma G.P., Shreenivas A., Shukla M., Himburg H.A.) Head & Neck. 2022 March 08. https://doi.org/10.1002/hed.27028
Effect of the environment on home-based self-sampling kits for anal cancer screening. (Nitkowski J., Giuliano A., Ridolfi T., Chiao E., Fernandez M.E., Schick V., Swartz M.D., Smith J.S., Schneider E.A., Brzezinski B., Nyitray A.G.) Journal of Virological Methods. 2022 December. https://doi.org/10.1016/j.jviromet.2022.114616
Plasma tissue-type plasminogen activator is associated with lipoprotein(a) and clinical outcomes in hospitalized patients with COVID-19. (Zhang Z., Dai W., Zhu W., Rodriguez M., Lund H., Xia Y., Chen, Y., Rau M., Schneider E.A., Graham M.B., Jobe S., Wang D., Cui W., Wen R., Whiteheart S.W., Wood J.P., Silverstein R., Berger J.S., Baumann Kreuziger L., Barrett T.J., Zheng Z.) Research and Practice in Thrombosis and Haemostasis. 2023 August. https://doi.org/10.1016/j.rpth.2023.102164
Profibrotic COVID-19 subphenotype exhibits enhanced localized ER-dependent HSP47+ expression in cardiac myofibroblasts in situ. (Jacobs E.R., Ross G.R, Padilla N., Pan A.Y., Liegl M.A., Puzyrenko A., Lai S., Dai Q., Uche N., Rubenstein J.C., North P.E., Ibrahim E.H., Sun Y., Felix J.C., Rui H., Benjamin I.J.) Journal of Molecular and Cellular Cardiology. 2023 December. https://doi.org/10.1016/j.yjmcc.2023.10.006
Keratinocyte Piezo1 drives paclitaxel-induced mechanical hypersensitivity. (Mikesell A.R., Isaeva E., Schulte M.L., Menzel A.D., Sriram A., Prahl M.M., Shin S.M., Sadler K.E., Yu H., Stucky C.L.) bioRxiv. 2023 December 13. https://doi.org/10.1101/2023.12.12.571332
Comparison of Absolute Expression and Turnover Number of COX-1 and COX-2 in Human and Rodent Cells and Tissues. (Li L., Sun R., Zenga J., Himburg H., Wang L., Duan S., Liu J., Bui D., Xie Z., Du T., Xie L., Yin T., Wong S., Gao S., Hu M.) Journal of Inflammation Research. 2022 August 04. https://doi.org/10.2147/JIR.S365842
Collagen‐Specific HSP47+ Myofibroblasts and CD163+ Macrophages Identify Profibrotic Phenotypes in Deceased Hearts With SARS‐CoV‐2 Infections. (Puzyrenko A., Jacobs E.R., Padilla N., Devine A., Aljadah M., Gantner B.N., Pan A.Y., Lai S., Dai Q., Rubenstein J.C., North P.E., Simpson P.M., Willoughby R.E., O’Meara C.C., Flinn M.A., Lough J.W., Ibrahim E., Zhen Z., Sun Y., Felix J., Hunt B.C., Ross G., Rui H., Benjamin I.J.) Journal of the American Heart Association. 2023 February 21. https://www.ahajournals.org/doi/10.1161/JAHA.122.027990
Mitochondrial-targeted antioxidant attenuates preeclampsia-like phenotypes induced by syncytiotrophoblast-specific GN1q signaling. (Opichka M.A., Livergood C.M., Balapattabi K., Ritter M.L., Brozoski D.T., Wackman K.K., Lu K., Kozak, K.N., Wells C., Fogo A.B., Gibson-Corley K.N., Kwitek A.E., Sigmund C.D., McIntosh J.J., Grobe J.L.) Science Advances. 2023 December 01. https://www.science.org/doi/10.1126/sciadv.adg8118
O-GlcNAc transferase contributes to sex-specific placental deregulation in gestational diabetes. (Cui Y., Cruz M., Palatnik A., Olivier-Van Stichelen S.) Placenta. 2023 January. https://doi.org/10.1016/j.placenta.2022.11.006.