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Research Bench Lab

MCW Tissue Bank - Information For Researchers

Pathology MCW Tissue Bank For Researchers

Overview

The MCW Tissue Bank is a secure storage facility that collects, processes and distributes blood and tissue for research on campus.

The MCW Tissue Bank is a College of American Pathologists accredited biorepository. Our protocols and services are rigorously assessed and validated to ensure the highest standards are met.

Services

Pathology MCW Tissue Bank For Researchers Services
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Biofluids

Services

  • Buffy coat lysis
  • PBMC processing
  • Simple blood processing
  • Cell counting

Samples

  • Whole blood – fresh or frozen
  • Plasma – fresh or frozen
  • Buffy coat – fresh or frozen
  • Cord blood – fresh
  • Plasma from cord blood – fresh or frozen
  • Buffy coat from cord blood – fresh or frozen
  • PBMCs
  • Serum from recovered COVID-19 blood samples – fresh or frozen
  • Bone Marrow MNCs (lysed discard samples from flow cytometry) – fresh or frozen
  • Fresh bone marrow (acquired from surgical resections)
Tissue

Services

  • Fresh tissue procurement
  • Snap freezing

Samples

  • Fresh tissue
  • Snap frozen tissue
  • Formalin fixed paraffin embedded FFPE) tissue
  • Frozen tissue shaves
Storage, supplies, and other supplies
  • Data logging and labeling samples
  • Monitored, secure -80°C storage
  • Media, storage containers, and laboratory supplies
Other Services

Other services available upon request.

Histocytometry Services

Tissue Microarrays (TMA)

Standard core-based tissue microarrays (TMA)

Cutting-Edge Matrix Assembly (CEMA)

Tissue arrays by Cutting-Edge Matrix Assembly (CEMA)

Cell Pellet Array

Core-based cell pellet microarray

Multicolor Immunofluorescence Histo-cytometry

Tumor biomarker analytic support: Multicolor quantitative immunofluorescence histo-cytometry

MCW Tissue Bank Programs

General Tissue Bank

General Tissue Bank

Consent Form

This is a lifetime consent and we consent for:

  • One additional blood draw during a regularly scheduled clinic lab draw
  • Otherwise discarded tissue from any future surgery, including bone marrow, tumor and control tissue

Availability to query available samples and deidentified clinical data with the help of CTSI’s i2b2 Cohort Discovery Tool. Deidentified clinical information we can provide:

  • Race
  • Age
  • Pathological diagnosis

Participation Information

To participate in the MCW Tissue Bank subjects must:

  • Be over 18 years of age
  • Be a patient of Froedtert & the Medical College of Wisconsin
  • Speak and read English (consent form is only available in English currently)
Learn more about the General Tissue Bank
Maternal Research, Placenta & Cord Blood Bank

Maternal Research, Placenta & Cord Blood Bank

Consent Form

We consent each pregnancy for:

  • Peripheral blood draw – usually takes place at approximately 20 weeks, but can be collected postpartum
  • Cord blood at time of delivery
  • Placenta and cord blood

Participation Information

To participate in the MCW Tissue Bank subjects must:

  • Be over 18 years of age
  • Be a patient delivering at Froedtert & the Medical College of Wisconsin
  • Speak and read English (consent form is only available in English currently)
Learn more about the Maternal Research, Placenta & Cord Blood Bank
Immuno-Oncology Bone Marrow Bank

Immuno-Oncology Bone Marrow Bank

Consent Form

This is a lifetime consent, and we consent for:

  • An additional bone marrow sample to be drawn for research purposes only, at any of the consented participants clinically ordered bone marrow biopsies or aspirates.
  • A paired blood sample to be drawn for research purposes only at any regularly scheduled blood draw

Availability to query available samples and deidentified clinical data with the help of CTSI’s i2b2 Cohort Discovery Tool. De identified clinical information we can provide:

  • Race
  • Age
  • Pathological diagnoses

Participation Information

To participate in the MCW Immuno-Oncology Bone Marrow Bank subjects must:

  • Be 18 years of age or older
  • Speak and read English (consent form is currently only available in English)
  • Be a patient of Froedtert and the Medical College of Wisconsin
  • Have a bone marrow biopsy or aspirate scheduled through Froedtert and the Medical College of Wisconsin
Learn more about the Immuno-Oncology Bone Marrow Bank

MCW Tissue Bank Request & Distribution Process

Request via iLab

All requests for MCW Tissue Bank services or samples should be made through iLab, which allows researchers to order, track, and pay for core services in a convenient, one-stop location. A fee schedule for MCW Tissue Bank services and samples can be found in iLab. To register your study in iLab, click the Register link below. Then complete and submit the Specimen/Service Request Form.

Register in iLab

Committee Approval Process

Once your request has been made via iLab, the MCW Tissue Bank Manager will provide you with a quote for the services provided. When approved by the study team, the request is sent to the MCW Tissue Bank’s Utilization Committee, and if needed, the Executive Committee. Once the request is approved by Committee members, the decision will be communicated to the study PI.

Distribution Process

After Committee approval, MCW Tissue Bank staff will work on completing the study request. There is an approximate 2 week turnaround time for most requests.

Inquiry Form

If you are not ready to submit an iLab request and have general questions regarding MCW Tissue Bank services and samples, please fill out the Inquiry Form below. Inquires must be made by MCW faculty and their study team.

MCW Tissue Bank Inquiry Form

Publications

The following hyperlinks go to publications that utilized MCW Tissue Bank services rather than specimens:

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2020

Keratinocytes contribute to normal cold and heat sensation. (Sadler KE, Moehring, F, Stucky CL.) Elife. 2020 Jul 30 PMID: 32729832 https://doi.org/10.7554/eLife.58625

Different Human Immune Lineage Compositions Are Generated in Non-Conditioned NBSGW Mice Depending on HSPC Source. (Hess N.J., Lindner P.N., Vazquez J., Grindel S., Hudson A.W., Stanic A.K., Ikeda A., Hematti P., Gumperz J.E.) Frontiers in Immunology. 2020 October 18 https://doi.org/10.3389/fimmu.2020.573406

GATA4, expressed in Barrett’s esophagus and esophageal adenocarcinoma, can block squamous epithelial cell gene expression in human esophageal cells. (Stavniichuk R, DeLaForest A., Thompson C.A., Miller J., Souza R.F., Battle M.A.) bioRxiv. 2020 June 18 https://doi.org/10.1101/2020.06.17.156026

H+/K+ATPase Expression in the Larynx of Laryngopharyngeal Reflux and Laryngeal Cancer Patients. (McCormick C.A., Samuels T.L., Battle M.A., Frolkis T., Blumin J.H., Bock J.M., Wells C., Yan K., Altman K.W., Johnston N.) Laryngoscope. 2020 April 06 https://doi.org/10.1002/lary.28643

Sensitive and specific immunohistochemistry protocols for detection of SARS-CoV-2 nucleocapsid and spike proteins in formalin-fixed, paraffin-embedded COVID-19 patient tissues. (Sun Y, Ge L, Rau MJ, et al. ) Research Square; 2020. https://doi.org/10.21203/rs.3.pex-1011/v1

2021

Diffuse interstitial pneumonia-like/macrophage activation syndrome-like changes in patients with COVID-19 correlate with length of illness. (Felix J.C., Sheinin Y.M., Suster D., Ronen N., Ratiani M., Vanden Heuvel T., Winge E., Patton M.D., Rau M.J., Ge L., Sun Y., Udhane S.S., Langenheim J.F., Rui H.) Annals of Diagnostic Pathology. 2021 August. https://doi.org/10.1016/j.anndiagpath.2021.151744

Twist1 signaling in age-dependent decline in angiogenesis and lung regeneration. (Hendee K, Hunyenyiwa T, Matus K, Toledo M, Mammoto A, Mammoto T. ) Aging (Albany NY). 2021 Mar 25;13(6):7781-7799. Epub 2021 Mar 25. PMID: 33764901; PMCID: PMC8034921 https://doi.org/10.18632/aging.202875 

GATA4 blocks squamous epithelial cell gene expression in human esophageal squamous cells. (Stavniichuk, R., DeLaForest, A., Thompson, C.A. et al.) Sci Rep 11, 3206 (2021). https://doi.org/10.1038/s41598-021-82557-x

Hypertension preserves the magnitude of microvascular flow-mediated dilation following transient elevation in intraluminal pressure. (Hughes W.E., Zinkevich N., Gutterman D.D., Beyer A.M.) Physiological Reports. 2021 February 15. https://doi.org/10.14814/phy2.14507

Pneumocytes are distinguished by highly elevated expression of the ER stress biomarker GRP78, a co-receptor for SARS-CoV-2, in COVID-19 autopsies. (Puzyrenko, A., Jacobs, E.R., Sun, Y. et al.) Cell Stress and Chaperones. 2021 August 12. https://doi.org/10.1007/s12192-021-01230-4

An Improved Detection of Circulating Tumor DNA in Extracellular Vesicles-Depleted Plasma. (Sun L., Du M., Kohli M., Huang C., Chen X., Xu M., Shen H., Wang S., Wang L.) Frontiers in Oncology. 2021 June 10. https://doi.org/10.3389/fonc.2021.691798 

Targeted biologic inhibition of both tumor cell-intrinsic and intercellular CLPTM1L/CRR9-mediated chemotherapeutic drug resistance. (Parashar, D., Geethadevi, A., McAllister, D. et al. ) npj Precis. Onc. 5, 16 (2021). https://doi.org/10.1038/s41698-021-00152-9

2022

Keratinocyte PIEZO1 modulates cutaneous mechanosensation. (Mikesell A.R., Isaeva O., Moehring F., Sadler K.E., Menzel A.D., Stucky C.L.) eLife. 2022 September 2. https://doi.org/10.7554/eLife.65987

Chronic stress promotes an immunologic inflammatory state and head and neck cancer growth in a humanized murine model. (Zenga J., Musaddiq A.J., Frei A., Petrie E., Sharma G.P., Shreenivas A., Shukla M., Himburg H.A.) Head & Neck. 2022 March 08. https://doi.org/10.1002/hed.27028

Comparison of Absolute Expression and Turnover Number of COX-1 and COX-2 in Human and Rodent Cells and Tissues. (Li L., Sun R., Zenga J., Himburg H., Wang L., Duan S., Liu J., Bui D., Xie Z., Du T., Xie L., Yin T., Wong S., Gao S., Hu M.) Journal of Inflammation Research. 2022 August 04. https://doi.org/10.2147/JIR.S365842

Effect of the environment on home-based self-sampling kits for anal cancer screening. (Nitkowski J., Giuliano A., Ridolfi T., Chiao E., Fernandez M.E., Schick V., Swartz M.D., Smith J.S., Schneider E.A., Brzezinski B., Nyitray A.G.) Journal of Virological Methods. 2022 December. https://doi.org/10.1016/j.jviromet.2022.114616

2023

Plasma tissue-type plasminogen activator is associated with lipoprotein(a) and clinical outcomes in hospitalized patients with COVID-19. (Zhang Z., Dai W., Zhu W., Rodriguez M., Lund H., Xia Y., Chen, Y., Rau M., Schneider E.A., Graham M.B., Jobe S., Wang D., Cui W., Wen R., Whiteheart S.W., Wood J.P., Silverstein R., Berger J.S., Baumann Kreuziger L., Barrett T.J., Zheng Z.) Research and Practice in Thrombosis and Haemostasis. 2023 August. https://doi.org/10.1016/j.rpth.2023.102164

Profibrotic COVID-19 subphenotype exhibits enhanced localized ER-dependent HSP47+ expression in cardiac myofibroblasts in situ. (Jacobs E.R., Ross G.R, Padilla N., Pan A.Y., Liegl M.A., Puzyrenko A., Lai S., Dai Q., Uche N., Rubenstein J.C., North P.E., Ibrahim E.H., Sun Y., Felix J.C., Rui H., Benjamin I.J.) Journal of Molecular and Cellular Cardiology. 2023 December. https://doi.org/10.1016/j.yjmcc.2023.10.006

Keratinocyte Piezo1 drives paclitaxel-induced mechanical hypersensitivity. (Mikesell A.R., Isaeva E., Schulte M.L., Menzel A.D., Sriram A., Prahl M.M., Shin S.M., Sadler K.E., Yu H., Stucky C.L.) bioRxiv. 2023 December 13. https://doi.org/10.1101/2023.12.12.571332

Collagen‐Specific HSP47+ Myofibroblasts and CD163+ Macrophages Identify Profibrotic Phenotypes in Deceased Hearts With SARS‐CoV‐2 Infections. (Puzyrenko A., Jacobs E.R., Padilla N., Devine A., Aljadah M., Gantner B.N., Pan A.Y., Lai S., Dai Q., Rubenstein J.C., North P.E., Simpson P.M., Willoughby R.E., O’Meara C.C., Flinn M.A., Lough J.W., Ibrahim E., Zhen Z., Sun Y., Felix J., Hunt B.C., Ross G., Rui H., Benjamin I.J.) Journal of the American Heart Association. 2023 February 21. https://www.ahajournals.org/doi/10.1161/JAHA.122.027990

Mitochondrial-targeted antioxidant attenuates preeclampsia-like phenotypes induced by syncytiotrophoblast-specific GN1q signaling. (Opichka M.A., Livergood C.M., Balapattabi K., Ritter M.L., Brozoski D.T., Wackman K.K., Lu K., Kozak, K.N., Wells C., Fogo A.B., Gibson-Corley K.N., Kwitek A.E., Sigmund C.D., McIntosh J.J., Grobe J.L.) Science Advances. 2023 December 01. https://www.science.org/doi/10.1126/sciadv.adg8118

O-GlcNAc transferase contributes to sex-specific placental deregulation in gestational diabetes. (Cui Y., Cruz M., Palatnik A., Olivier-Van Stichelen S.) Placenta. 2023 January. https://doi.org/10.1016/j.placenta.2022.11.006.

2024

Genome-Wide Association Study Identifies Novel Loci Associated with Survival in Multiple Myeloma. (Lee, Remy; Arsang-Jang, Shahram; D'Souza, Anita; Dhakal, Binod; Janz, Siegfried; Dong, Jing) Blood. 2024 December 6. DOI: https://doi.org/10.1182/blood-2024-201795

379 Characterizing the single-cell transcriptomes of fetal natural killer cells isolated from the umbilical cord of fetuses exposed to human cytomegalovirus during gestation. (Khalil, Mohamed; Terhune, Scott; Malarkannan, Subramaniam) Journal of Clinical and Translational Science. 2024 April 03. DOI: https://doi.org/10.1017/cts.2024.333

Type I interferon signaling promotes radioresistance in head and neck cancer. (Zenga J, Awan MJ, Frei A, Massey B, Bruening J, Shukla M, Sharma GP, Shreenivas A, Wong SJ, Zimmermann MT, Mathison AJ, Himburg HA.) Transl Cancer Res. 2024;13(5):2535-2543. doi: 10.21037/tcr-23-2104

Increased keratinocyte activity and PIEZO1 signaling contribute to paclitaxel-induced mechanical hypersensitivity. (Mikesell, Alexander R; Isaeva, Elena; Schulte, Marie L; Menzel, Anthony D; Sriram, Anvitha; Prahl, Megan M; Shin, Seung Min; Sadler, Katelyn E; Yu, Hongwei; Stucky, Cheryl L) Science Translational Medicine. 2024 December 11. DOI: https://doi.org/10.1126/scitranslmed.adn5629

Concordance of Human Papillomavirus Genotypes in Mailed Home-Based Self-Collected Versus Clinician-Collected Anal Swabs Among Sexual and Gender Minority Individuals. (Nitkowski, Jenna PhD; Giuliano, Anna R. PhD; Ridolfi, Tim MD; Chiao, Elizabeth MD, MPH; Fernandez, Maria E. PhD; Schick, Vanessa PhD; Swartz, Michael D. PhD; Smith, Jennifer S. PhD; Nyitray, Alan G. PhD.) Sexually Transmitted Diseases 51(4):p 270-275, April 2024. DOI: 10.1097/OLQ.0000000000001916

Recent Progress in mesenchymal stem cell-based therapy for acute lung injury. (Liang, Jinfeng; Dai, Weiyou; Xue, Shihang; Wu, Feifei; Cui, Enhai; Pan, Ruolang) Cell and Tissue Banking 25 (2):677-684. 2024. https://doi.org/10.1007/s10561-024-10129-0

Deep learning for automated detection of breast cancer in deep ultraviolet fluorescence images with diffusion probabilistic model. (Ghahfarokhi, Sepehr Salem; To, Tyrell; Jorns, Julie; Yen, Tina; Yu, Bing; Ye, Dong Hye) 2024 IEEE International Symposium on Biomedical Imaging (ISBI). 2024 August 22. https://doi.org/10.1109/ISBI56570.2024.10635349

Acceptability of anal human papillomavirus home self-sampling and clinician sampling among sexual and gender minority individuals in Milwaukee, Wisconsin: The prevent anal cancer self-swab study. (Nitkowski, Jenna; Giuliano, Anna R; Ridolfi, Tim; Chiao, Elizabeth; Fernandez, Maria E; Schick, Vanessa; Swartz, Michael D; Smith, Jennifer S; Nyitray, Alan G) LGBT Health. 2024 January 16. https://doi.org/10.1089/lgbt.2023.0012

Biofabrication of Small Vascular Graft with Acellular Human Amniotic Membrane: A Proof-of-Concept Study in Pig. (Aung, O; Rossi, Peter J; Dyer, Mitchell R; Stellpflug, Austin; Zhai, Yingnan; Kenneth, Allen; Wang, Xiaolong; Chang, Jackie; Chen, Yiliang; Tefft, Brandon) bioRxiv. 2024 September 15. https://doi.org/10.1101/2024.09.11.612466

Inhibition of angiogenesis and regenerative lung growth in Lepob/ob mice through adiponectin-VEGF/VEGFR2 signaling. (Hunyenyiwa, Tendai; Kyi, Priscilla; Scheer, Mikaela; Joshi, Mrudula; Gasparri, Mario; Mammoto, Tadanori; Mammoto, Akiko) Frontiers in Cardiovascular Medicine. 2024 October 16. https://doi.org/10.3389/fcvm.2024.1491971

Chromatin State Maps of Blood Pressure-Relevant Renal Segments Reveal Potential Regulatory Role for SNPs. (Ray, Atrayee; Yang, Chun; Stelloh, Cary; Tutaj, Monika; Liu, Pengyuan; Liu, Yong; Qiu, Qiongzi; Auer, Paul L; Lin, Chien-Wei; Widlansky, Michael E.) Hypertension. 2024 December 26. https://doi.org/10.1161/HYPERTENSIONAHA.124.23873

Studying the O-GlcNAcome of human placentas using banked tissue samples. (Sarai Luna, Florian Malard, Michaela Pereckas, Mayumi Aoki, Kazuhiro Aoki, Stephanie Olivier-Van Stichelen) Glycobiology. 2024 April. cwae005, https://doi.org/10.1093/glycob/cwae005

The impact of body mass index and physical disability on home-based anal self-sampling. (Nitkowski, J., Fernandez, M.E., Ridolfi, T. et al.) Cancer Causes Control. 2024 January. https://doi.org/10.1007/s10552-023-01775-y

2025

460 Fetal natural killer cells play an essential immunoprotective role in preventing the onset of symptomatic congenital cytomegalovirus. (Khalil, Mohamed; Terhune, Scott; Malarkannan, Subramaniam) Journal of Clinical and Translational Science. 2025 April 11. https://doi.org/10.1017/cts.2024.1054

Semiautomated mapping of tumor annotations from H&E to fluorescence images of human breast surgical tissues. (Niu, Tianling; Ampo, Emi; Jorns, Julie; Patton, Mollie; Lu, Tongtong; Ye, Dong H; Yen, Tina; Yu, Bing) Multimodal Biomedical Imaging XX. 2025 March 20. https://doi.org/10.1117/12.3041374

Hepatocyte reporter cells and integrated metabolomic and transcriptomic analyses reveal insights into hepatocyte changes in offspring of pregnancies with obesity. (Kozlovich, Svetlana Y; Pan, Amy Y; McIntosh, Jennifer J; Palatnik, Anna; Jia, Shuang; Hessner, Martin J; Nghiem-Rao, T Hang) Scientific Reports. 2025 July 25. https://doi.org/10.1038/s41598-025-10808-2

Fetal macrosomia and placental expression of fibroblast growth factor 21 and peroxisome proliferator-activated receptor alpha. (Harrison, R. K., Allen, K., Naatz, A., Corbett, J., McIntosh, J., & Cruz, M.) Systems Biology in Reproductive Medicine. 2025 May 24. https://doi.org/10.1080/19396368.2025.2504450

Patient-Informed Fluid-Structure Interaction Simulations of Bicuspid Aortic Valve in Young Adults Reveal Regionalized Differences in Mechanical Stress. (Kazik, Hail B; Kandail, Harkamaljot S; Lincoln, Joy; LaDisa, John F.) Ann Biomed Eng. 2025 November 24. https://doi.org/10.1007/s10439-025-03919-4

Enhanced protein extraction and quantification protocol for microsamples: An ultra‐sensitive workflow for low‐volume, low‐concentration total protein lysates. (Wilcox, Taylor; Widlansky, Michael E; Westhoff, Justin; Wang, Jingli; Ying, Rong; Thorgerson, Abigail; Roberts, Michelle L.) Protein Science. 2025 May 15. https://doi.org/10.1002/pro.70161

Breast Cancer Classification in Deep Ultraviolet Fluorescence Images Using a Patch-Level Vision Transformer Framework. (Afshin, Pouya; Helminiak, David; Lu, Tongtong; Yen, Tina; Jorns, Julie M; Patton, Mollie; Yu, Bing; Ye, Dong Hye) arXiv preprint arXiv:2505.07654. 2025 May 12. https://doi.org/10.48550/arXiv.2505.07654

Transforming Human Amniotic Membrane into Nanoparticles: An Approach for Cardiac Delivery Systems. (Stellpflug, Austin; Li, Siqi; Wan, Tina; Mattern, Carol; Nguyen, Christopher; Xu, Dongzhu; Han, Lu; Wang, Bo) ACS Applied Bio Materials. 2025 August 11. https://doi.org/10.1021/acsabm.5c00920

Mindfulness vs. sleep education during autologous hematopoietic cell transplantation for multiple myeloma: Feasibility of a randomized controlled pilot study. (Henley, E. C., Liphart, H. A., Morris, K. J., Awoyinka, I., Irwin, M. R., Costanzo, E. S., ... & Knight, J. M.) Contemporary Clinical Trials Communications.2025. October. https://doi.org/10.1016/j.conctc.2025.101540

Role of cGAS/STING pathway in aging and sexual dimorphism in diabetic kidney disease. (Khedr, Sherif, Lashodya V. Dissanayake, Ammar J. Alsheikh, Adrian Zietara, Denisha R. Spires, Romica Kerketta, Angela J. Mathison, Raul Urrutia, Oleg Palygin, and Alexander Staruschenko) JCI insight. 2024. https://doi.org/10.1172/jci.insight.174126

Effective transfer of tumor annotations from hematoxylin and eosin to fluorescence images of breast and lung tissues. (Tianling Niu, Emi Ampo, Julie M. Jorns, Mollie Patton, Tongtong Lu, Dong Hye Ye, Tina W. F. Yen, and Bing Yu) J. Biomed. Opt. 31(1) 016501 (24 December 2025) https://doi.org/10.1117/1.JBO.31.1.016501

2026

The Synthetic Extracellular Matrix as a Maestro of the In Vitro Stem Cell Niche: Orchestrating Fate and Function. (Giri S, Rajesh P.) Biomedicines. 2026; 14(2):485. https://doi.org/10.3390/biomedicines14020485