Medical College of Wisconsin Analytical Cores

Biochemical Assay Core
The Biochemical Assay Core provides a consolidated, highly specialized, well equipped and professionally staffed analytical laboratory capable of performing a wide variety of immunoassays, clinical chemistry and biochemical analyses to meet the analytical needs of members of the Department of Physiology and their collaborators.

Director: Gary Mouradian, PhD

Biochemistry Shared Research Instrumentation
The Biochemistry Department maintains several instruments for isolation and physical characterization of biomolecules and detection of their interactions.

Director: Brian Smith, PhD

CRI Shared Equipment
Advanced equipment for single cell (Chromium Controller, Chromium X) sequencing preparation and spatial genomics (Visium CytAssist) for self-use.

Director: Nick Kampa

High-Field Nuclear Magnetic Resonance (NMR) Facility
The NMR Facility is an interdepartmental research service unit located in the Biochemistry Department. High-field NMR spectroscopy is a powerful technique for the study of biomolecular structure and dynamics. The facility provides service for routine 1D and 2D NMR methods, and can also provide consultation and collaborative assistance with the acquisition and analysis of multidimensional, multinuclear protein NMR spectra.

Director: Francis Peterson, PhD

Translational Metabolism Shared Resource (TraMSR)
The Translational Metabolomics Shared Resource (TraMSR) (Redox & Bioenergetics, Preclinical Biomedical Imaging, Mass Spectrometry, Radiology Quantitative Imaging) provides expertise and state-of-the-art instrumentation for MCW investigators and cancer center members to explore cancer and non-cancer metabolism by enabling users to run mass spectrometry analyses, bioenergetic and redox function analyses, pre-clinical imaging and therapy response.

Director: Karin Hoffmeister, MD

TraMSR Biomedical Imaging
The Biomedical Imaging Shared Resource (BISR) advances research at MCW by providing expertise and state-of-the-art technology and methods for preclinical bioluminescence and fluorescence, low dose X ray microCT and Cherenkov Luminescence imaging. Preclinical near-infrared (NIR) and short-wave infrared imaging (SWIR) greatly increasing the translational potential of non-cancer and cancer center research.

Director: Amit Joshi, PhD; Manager: Shayan Shafiee, PhD

TraMSR Mass Spectrometry
The TraMSR Mass Spectrometry Shared Resource provides multiple analytical services for proteins, peptides, lipids, small molecules, metabolites, complex glycans and other biological molecules to investigators at Medical College of Wisconsin and our partner institutions.

Director: Kazuhiro Aoki, PhD; Manager: Michaela Pereckas, BS

TraMSR Radiology Quantitative Imaging (QIL)
The Department of Radiology, in collaboration with the Medical College of Wisconsin’s Clinical Cancer Center (MCWCC) Clinical Trials Office, supports the assessment of tumor response to therapy guided by board certified radiologists. The QIL support clinical trials using Precision Imaging Metrics software to track and maintain clinical trial response criteria.

Co-Directors: Peter LaViolette, PhD and Stephanie Vincent-Sheldon, MD; Quality Analysts: Maureen Levenhagen and Allie Lowman

TraMSR Redox and Bioenergetics
Metabolic services supported by the Redox and Bioenergetics Shared Resource (RBSR) include reactive oxygen species(ROS) measurement, metabolic pathway profiling, oxygen consumption, glycotic function, membrane potential and metabolic reprograming using Agilent Seahorse , HPLC and UHPLCelectron paramagnetic resonance (EPR) and luminescence spectrometers.

Director: Jacek Zielonka, PhD; Manager: Monika Zielonka, BS