Kirby Laboratory
Location
Microbiology & Immunology
BSB B2410
General Interests
Bacterial Signal Transduction, Microbial Communities, Predator-Prey Dynamics, Xenobiotic Disruption of Microbiomes
Research Areas
Microbiome
We are examining the role of xenobiotics for their capacity to disrupt the gut microbiota with deleterious consequences on host physiology and metabolism. Disease models used in the lab are a salt-sensitive rat model for hypertension, a mouse model of diet-induced obesity, and a mouse model of obesity induced by the second-generation antipsychotic risperidone. We perform physiological measurements related to each disease model, fecal material transfers to establish a causal link between the microbiota and disease, and 16S rDNA and metagenomic sequencing to analyze taxonomic and functional composition of the microbiota. Metagenomic analyses allow us to identify and test candidate probiotic strains of bacteria with specific metabolic features that influence the progression of disease. We have filed a patent (US 2020/0016124 A1) based on our work leading to reduction of weight gain and are in discussions to create a startup company.
Predator-Prey Interactions
The above work is a direct product of our understanding of bacterial interactions between Myxococcus xanthus and Bacillus subtilis as a model for microbial predator-prey interactions. Our primary goal is to assess the role of specialized metabolites produced by both the predator and the prey. Detailed molecular mechanisms for predator-prey interactions in vitro provide insights into gut-based systems in rodents and humans.
Current Members
Recent Publications
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(Fernando DG, Saravia FL, Atkinson SN, Barron M, Kirby JR, Kindel TL.) Surg Endosc. 2023 Feb;37(2):1476-1486 PMID: 35768736 SCOPUS ID: 2-s2.0-85133186635 06/30/2022
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Nicotinamide Riboside-Conditioned Microbiota Deflects High-Fat Diet-Induced Weight Gain in Mice.
(Lozada-Fernández VV, deLeon O, Kellogg SL, Saravia FL, Hadiono MA, Atkinson SN, Grobe JL, Kirby JR.) mSystems. 2022 Feb 22;7(1):e0023021 PMID: 35076278 PMCID: PMC8788325 01/26/2022
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(Reho JJ, Nakagawa P, Mouradian GC Jr, Grobe CC, Saravia FL, Burnett CML, Kwitek AE, Kirby JR, Segar JL, Hodges MR, Sigmund CD, Grobe JL.) Front Physiol. 2022;13:855054 PMID: 35283781 PMCID: PMC8914175 SCOPUS ID: 2-s2.0-85126220642 03/15/2022
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Sleeve gastrectomy prevents hypertension associated with unique shifts in the gut microbiome.
(Barron M, Atkinson SN, Kirby J, Kindel T.) Surg Endosc. 2021 Oct;35(10):5461-5467 PMID: 32989546 PMCID: PMC8004532 SCOPUS ID: 2-s2.0-85091609559 09/30/2020
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(Abais-Battad JM, Saravia FL, Lund H, Dasinger JH, Fehrenbach DJ, Alsheikh AJ, Zemaj J, Kirby JR, Mattson DL.) Acta Physiol (Oxf). 2021 Aug;232(4):e13662 PMID: 33866692 PMCID: PMC9835005 SCOPUS ID: 2-s2.0-85104879371 04/19/2021
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Gut Microbiota Represent a Major Thermogenic Biomass.
(Riedl RA, Burnett CML, Pearson NA, Reho JJ, Mokadem M, Edwards RA, Kindel TL, Kirby JR, Grobe JL.) Function (Oxf). 2021;2(3):zqab019 PMID: 33939772 PMCID: PMC8055641 05/04/2021
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(Pickrum AM, DeLeon O, Dirck A, Tessmer MH, Riegert MO, Biller JA, Ledeboer NA, Kirby JR, Frank DW.) Infect Immun. 2020 Jun 22;88(7) PMID: 32366575 PMCID: PMC7309624 SCOPUS ID: 2-s2.0-85088536617 05/06/2020
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(Tiwari N, López-Redondo M, Miguel-Romero L, Kulhankova K, Cahill MP, Tran PM, Kinney KJ, Kilgore SH, Al-Tameemi H, Herfst CA, Tuffs SW, Kirby JR, Boyd JM, McCormick JK, Salgado-Pabón W, Marina A, Schlievert PM, Fuentes EJ.) Proc Natl Acad Sci U S A. 2020 May 19;117(20):10989-10999 PMID: 32354997 PMCID: PMC7245129 SCOPUS ID: 2-s2.0-85084961758 05/02/2020
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(Bretl DJ, Ladd KM, Atkinson SN, Müller S, Kirby JR.) PLoS Genet. 2018 Oct;14(10):e1007714 PMID: 30346960 PMCID: PMC6211767 SCOPUS ID: 2-s2.0-85055910992 10/23/2018
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Ancient Regulatory Role of Lysine Acetylation in Central Metabolism.
(Nakayasu ES, Burnet MC, Walukiewicz HE, Wilkins CS, Shukla AK, Brooks S, Plutz MJ, Lee BD, Schilling B, Wolfe AJ, Müller S, Kirby JR, Rao CV, Cort JR, Payne SH.) mBio. 2017 Nov 28;8(6) PMID: 29184018 PMCID: PMC5705920 SCOPUS ID: 2-s2.0-85039922037 12/01/2017
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The Gut Microbiome, Energy Homeostasis, and Implications for Hypertension.
(Riedl RA, Atkinson SN, Burnett CML, Grobe JL, Kirby JR.) Curr Hypertens Rep. 2017 Apr;19(4):27 PMID: 28316052 PMCID: PMC5773096 SCOPUS ID: 2-s2.0-85015782153 03/21/2017
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(Kelly PH, Bahr SM, Serafim TD, Ajami NJ, Petrosino JF, Meneses C, Kirby JR, Valenzuela JG, Kamhawi S, Wilson ME.) mBio. 2017 Jan 17;8(1) PMID: 28096483 PMCID: PMC5241394 SCOPUS ID: 2-s2.0-85014830318 01/18/2017